Taylor AFS, Hogbom L, Hogberg M, Lyon AJE, Nasholm T, Hogberg P
Natural N-15 abundance in fruit bodies of ectomycorrhizal fungi from boreal forests
New Phytologist: 1997 136:713-720
The N-15 natural abundance and N concentrations of fruit bodies from 70 species (23 genera) of ectomycorrhizal fungi found in boreal forests are presented. Large intraspecific and intrageneric differences were found, e.g. 8.3 parts per thousand N-15 in the species Dermocybe crocea and 12.6 parts per thousand in the genus Cortinarius. In addition, significant differences in both delta(15)N and %N were found between different parts of fruit bodies, with cap material giving consistently higher values. Proteins and amino acids were enriched by 9.7 +/- 0.4 parts per thousand (mean +/- SE) relative to chitin, irrespective of the part of the fruit body examined. Chitin had delta(15)N values similar to that of plant hosts. The higher delta(15)N and %N values of the caps than of the stipes probably reflect a higher portion of proteins and amino acids in the caps. The delta(15)N of mycorrhizal fungi can be a function of the N species used (organic N, NH4+, NO3-), the depth of soil at which the mycelium occurs, and metabolic fractionations. The metabolic fractionations, e.g. potential transaminations during the flux of N from the soil through the fungus to the plant, make it difficult, at present, to make inferences about sources of N based on delta(15)N values alone. No effect of sample drying temperature on delta(15)N values of fungal material was detected.
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