Project description

This stipend is offered as part of a collaborative project involving four groups from Umeå Plant Science Centre (Ljung, Hanson, Nilsson, Schmid) that will investigate the importance of the chromatin landscape and epigenetic regulation on the activity of the flowering time network and its crosstalk with hormone and sugar signaling pathways in A. thaliana and hybrid aspen at a tissue- and cell type specific level. The project will employ an interdisciplinary strategy based on state-of-the-art genomics, and genetics, as well as molecular and biochemical methodologies.

The successful candidate will participate in establishing methodology to assay the translation of mRNAs specifically in the cells of the shoot apical meristem of A. thaliana grown in different environments (temperature/light regimes) using immunoprecipitation of translating ribosomes followed by sequencing of translated mRNAs. The results will be compared to the results from mRNA abundance studies, proteomic investigation and changes of metabolite levels. Ultimately, understanding how plants perceive and respond to environmental perturbations will help us to predict the ecological consequences of future climate change and will assist in the development of new plant varieties that are better adapted to their particular environments.

Qualifications

The successful candidate must have a PhD degree or a foreign degree that is deemed equivalent in biology, biochemistry, biotechnology or equivalent subject, preferably with a specialization in plant physiology and/or genomics. To be eligible for the stipend, you should have completed your doctoral degree maximum 3 years before the end of the application period, unless special circumstances exist.

You must have good experience with standard molecular biology, plant genetics and related topics. Previous experience in genomics and basic high/throughput data analysis methods are merits. Good oral and writing skills in English are essential qualifications. The successful candidate is expected to have good social skills and the ability to do research independently, as well as in a team.

The fellowship is for 2-years tax-exempt with start 1st September 2017. The stipend is 291 600 SEK per year.

Molecular understanding of sugar mediated growth control
The overexpression of bZIP11, a transcription factor involved in the response to sugars, inhibits growth of Arabidopsis seedlings (Hanson et al., 2008). A transcriptomic approach has highlighted genes implicated in primary carbon metabolism as direct transcriptional targets of bZIP11 (Ma et al., 2011).

We recently showed that a mutation in these genes results in a partial to total rescue of the bZIP11 overexpression phenotype and therefore hypothesized that other mutations may as well result in the same rescue. To identify these mutants, an EMS mutagenesis has been performed on the bZIP11 overexpressing line suitable for a suppressor screen.

The aims of this project are to screen the M2 population deriving from this mutagenesis and to further characterize the mutants in physiological and molecular methodology.

Contact: Thomas Dobrenel (This email address is being protected from spambots. You need JavaScript enabled to view it.), Johannes Hanson (This email address is being protected from spambots. You need JavaScript enabled to view it.)