The unique photosynthetic apparatus of Pinaceae - Analysis of photosynthetic complexes in Norway spruce (Piceaabies)
J Exp Bot. 2019 Apr 2 Epub ahead of print]

The Dynamics of Cambial Stem Cell Activity
Annu Rev Plant Biol. 2019 Mar 1 [Epub ahead of print]

Regulatory Diversification of INDEHISCENT in the Capsella Genus Directs Variation in Fruit Morphology
Curr Biol. 2019, 29(6):1038-

Surveillance of cell wall diffusion barrier integrity modulates water and solute transport in plants
Sci Rep. 2019, 9(1):4227

Selective auxin agonists induce specific AUX/IAA protein degradation to modulate plant development
Proc Natl Acad Sci U S A. 2019, 116(13):6463-6472
Vain T, Raggi S, Ferro N, Barange DK, Kieffer M, Ma Q, Doyle SM, Thelander M, Pařízková B, Novák O, Ismail A, Enquist PA, Rigal A, Łangowska M, Ramans Harborough S, Zhang Y, Ljung K, Callis J, Almqvist F, Kepinski S, Estelle M, Pauwels L, Robert S

Abstract
Auxin phytohormones control most aspects of plant development through a complex and interconnected signaling network. In the presence of auxin, AUXIN/INDOLE-3-ACETIC ACID (AUX/IAA) transcriptional repressors are targeted for degradation by the SKP1-CULLIN1-F-BOX (SCF) ubiquitin-protein ligases containing TRANSPORT INHIBITOR RESISTANT 1/AUXIN SIGNALING F-BOX (TIR1/AFB). CULLIN1-neddylation is required for SCFTIR1/AFB functionality, as exemplified by mutants deficient in the NEDD8-activating enzyme subunit AUXIN-RESISTANT 1 (AXR1). Here, we report a chemical biology screen that identifies small molecules requiring AXR1 to modulate plant development. We selected four molecules of interest, RubNeddin 1 to 4 (RN1 to -4), among which RN3 and RN4 trigger selective auxin responses at transcriptional, biochemical, and morphological levels. This selective activity is explained by their ability to consistently promote the interaction between TIR1 and a specific subset of AUX/IAA proteins, stimulating the degradation of particular AUX/IAA combinations. Finally, we performed a genetic screen using RN4, the RN with the greatest potential for dissecting auxin perception, which revealed that the chromatin remodeling ATPase BRAHMA is implicated in auxin-mediated apical hook development. These results demonstrate the power of selective auxin agonists to dissect auxin perception for plant developmental functions, as well as offering opportunities to discover new molecular players involved in auxin responses.

e-link to publication
Dynamic pH-induced conformational changes of the PsbO protein in the fluctuating acidity of the thylakoid lumen
Physiol Plant. 2019 Feb 21 [Epub ahead of print]

Automation and Scale Up of Somatic Embryogenesis for Commercial Plant Production, With Emphasis on Conifers
FRONTIERS IN PLANT SCIENCE 2019, 10:109

Rho-of-plant-activated root hair formation requires Arabidopsis YIP4a/b gene function
Development. 2019, 146(5)

FT modulates genome-wide DNA-binding of the bZIP transcription factor FD
Plant Physiol. 2019 Feb 15 [Epub ahead of print]

Control of root meristem establishment in conifers
PHYSIOLOGIA PLANTARUM 2019, 165(1):81-89

Control of adventitious root formation: insights into synergistic and antagonistic hormonal interactions
PHYSIOLOGIA PLANTARUM 2019, 165(1): 90-100

Tissue-specific hormone profiles from woody poplar roots under bending stress
PHYSIOLOGIA PLANTARUM 2019, 165(1):101-113

Estimation of genetic parameters, provenance performances, and genotype by environment interactions for growth and stiffness in lodgepole pine (Pinus contorta)
SCANDINAVIAN JOURNAL OF FOREST RESEARCH 2019, 34(1):1-11

Extracellular peptide Kratos restricts cell death during vascular development and stress in Arabidopsis
J Exp Bot. 2019 Feb 7 [Epub ahead of print]

Dual and Dynamic Intracellular localisation of Arabidopsis thaliana SnRK1.1
J Exp Bot. 2019 Feb 7 [Epub ahead of print]

Genetic analysis of wood quality traits in Norway spruce open-pollinated progenies and their parent plus-trees at clonal archives, and the evaluation of phenotypic selection of plus-trees
Canadian Journal of Forest Research 2019, e-First Article

Can leaf net photosynthesis acclimate to rising and more variable temperatures?
Plant Cell Environ. 2019 Feb 1 [Epub ahead of print]

Heterologous phosphoketolase expression redirects flux towards acetate, perturbs sugar phosphate pools and increases respiratory demand in Saccharomyces cerevisiae
Microb Cell Fact. 2019, 18(1):25

Phloem companion cell-specific transcriptomic and epigenomic analyses identify MRF1, a novel regulator of flowering
Plant Cell. 2019, 31(2):325-345

Lateral Transport of Organic and Inorganic Solutes
Plants (Basel). 2019, 8(1)

Auxin Function in the Brown Alga Dictyota dichotoma
Plant Physiol. 2019, 179(1):280-299

UDP-Sugar Producing Pyrophosphorylases: Distinct and Essential Enzymes With Overlapping Substrate Specificities, Providing de novo Precursors for Glycosylation Reactions
Front. Plant Sci. 2019, 9:1822

A microstructured p-Si photocathode outcompetes Pt as a counter electrode to hematite in photoelectrochemical water splitting
Dalton Trans. 2018 Dec 11 [Epub ahead of print]

Environmentally Sensitive Molecular Switches Drive Poplar Phenology
Front Plant Sci. 2018 Dec 17;9:1873

A Tree Ortholog of SHORT VEGETATIVE PHASE Floral Repressor Mediates Photoperiodic Control of Bud Dormancy
Curr Biol. 2018, 29(1):128-+

Accuracy of genomic selection for growth and wood quality traits in two control-pollinated progeny trials using exome capture as the genotyping platform in Norway spruce
BMC Genomics. 2018, 19(1):946

Novel pipeline identifies new upstream ORFs and non-AUG initiating main ORFs with conserved amino acid sequences in the 5' leader of mRNAs in Arabidopsis thaliana
RNA. 2018: 25(3):292-304

AtLHT1 Transporter Can Facilitate the Uptake and Translocation of a Glycinergic-Chlorantraniliprole Conjugate in Arabidopsis thaliana
J Agric Food Chem. 2018, 66(47):12527-12535

The Inhibitor Endosidin 4 Targets SEC7 Domain-Type ARF GTPase Exchange Factors and Interferes with Subcellular Trafficking in Eukaryotes
PLANT CELL 2018, 30(10):2553-2572

Capturing complexity: Forests, decision-making and climate change mitigation action
GLOBAL ENVIRONMENTAL CHANGE-HUMAN AND POLICY DIMENSIONS 2018 52:238-247

Thermodynamic buffering, stable non-equilibrium and establishment of the computable structure of plant metabolism
Prog Biophys Mol Biol. 2018 Nov 13 [Epub ahead of print]

Improved and synchronized maturation of Norway spruce (Picea abies (L.) H.Karst.) somatic embryos in temporary immersion bioreactors
In Vitro Cell Dev Biol Plant. 2018, 54(6):612-620

Gene-edited plants: What is happening now?
Physiol Plant. 2018, 164(4):370-371

Analysis of phenotypic- and Estimated Breeding Values (EBV) to dissect the genetic architecture of complex traits in a Scots pine three-generation pedigree design
J Theor Biol. 2019, 462:283-292

Integrative Analysis of Three RNA Sequencing Methods Identifies Mutually Exclusive Exons of MADS-Box Isoforms During Early Bud Development in Picea abies
FRONTIERS IN PLANT SCIENCE 2018, 8:1625

Nitrogen utilization during germination of somatic embryos of Norway spruce: revealing the importance of supplied glutamine for nitrogen metabolism
Trees 2018, 33(2):383-394
Carlsson J, Egertsdotter U, Ganeteg U, Svennerstam H

Abstract
Key message
This paper shows that germinating Norway spruce somatic embryos are dependent on the carbon and nitrogen supplied in the medium, and that supplied glutamine accounts for 50 % of assimilated nitrogen during germination.

The female megagametophyte, which provides the zygotic embryo with nitrogen (N), carbon (C) and energy during germination, is not present in Norway spruce (Picea abies) mature somatic embryos. Therefore, somatic embryos presumably rely on nutrients supplied in the germination medium in addition to their storage compounds accumulated during maturation. However, to what extent stored versus supplied compounds contribute to a somatic embryo germination is unclear. In this 24-day study, we addressed the above question by monitoring the biomass changes and the N and C budget during somatic embryo germination, under low-intensity red light. We found that the C and N storage reserves, accumulated during the maturation phase, were not sufficient to support the growth of the germinating somatic embryos, rather they were dependent on the medium components. In addition, in a previous study it has been found that glutamine (Gln) supplied in the medium was crucial for maintaining the primary amino acid (AA) metabolism and growth of the proliferating embryogenic cultures of Norway spruce (Carlsson et al., PLoS One 12(8):e0181785, 2017). Therefore, we hypothesised that Gln would be required as a significant source of N also during somatic embryo germination. By tracing the uptake of isotopically labelled N-sources from the medium and further into primary N assimilation, we found that Gln was the preferred source of N for the germinating somatic embryos, accounting for 50% of assimilated N. As the amounts of both arginine (Arg) and Gln were increased in the germinating somatic embryos, it also suggested that germination in low-intensity red light promoted N storage, similar to what has been observed in the zygotic embryo maturation in conifers (King, Gifford, Plant Physiol 113:1125–1135, 1997).

e-link to publication
Genetic control of transition from juvenile to mature wood with respect to microfibril angle in Norway spruce (Picea abies) and lodgepole pine (Pinus contorta)
Canadian Journal of Forest Research 23 August 2018

An Evolutionarily Conserved Abscisic Acid Signaling Pathway Regulates Dormancy in the Liverwort Marchantia polymorpha
Curr Biol. 2018, 28(22):3691-3699

Independent yet overlapping pathways ensure the robustness and responsiveness of trans-Golgi network functions in Arabidopsis
Development. 2018, 145(21)

Synchrotron FTIR and Raman spectroscopy provide unique spectral fingerprints for Arabidopsis floral stem vascular tissues
J Exp Bot. 2018 Nov 8 [Epub ahead of print]

Photo-electrochemical hydrogen production from neutral phosphate buffer and seawater using micro-structured p-Si photo-electrodes functionalized by solution-based methods
SUSTAINABLE ENERGY & FUELS 2018, 2(19):2215-2223

Engineering Non-cellulosic Polysaccharides of Wood for the Biorefinery
FRONTIERS IN PLANT SCIENCE 2018, 9

The gut microbiome participates in transgenerational inheritance of low temperature responses in Drosophila melanogaster
FEBS Lett. 2018, 592(24):4078-4086

Functional and evolutionary genomic inferences in Populus through genome and population sequencing of American and European aspen
Proc Natl Acad Sci U S A. 2018, 115(46):E10970-E10978

Growth and survival relationships of 71 tree species with nitrogen and sulfur deposition across the conterminous U.S.
PLoS One. 2018, 13(10):e0205296

ATP compartmentation in plastids and cytosol of Arabidopsis thaliana revealed by fluorescent protein sensing
PNAS 2018, 115(45):E10778-E10787

A genetic network mediating the control of bud break in hybrid aspen
Nat Commun. 2018, 9(1):4173

An image analysis pipeline to analyze emerging cracks or adhesion defects in living tissues
Bio Protoc. 2018, Oct 5, 8(19): e3036

The Xerobranching Response Represses Lateral Root Formation When Roots Are Not in Contact with Water
Curr Biol. 2018, 28(19):3165-3173

Microbial community response to growing season and plant nutrient optimisation in a boreal Norway spruce forest
SOIL BIOLOGY & BIOCHEMISTRY 2018, 125:197-209