Long-distance turgor pressure changes induce local activation of plant glutamate receptor-like channels.
Grenzi, M., Buratti, S., Parmagnani, A. S., Abdel Aziz, I., Bernacka-Wojcik, I., Resentini, F., Šimura, J., Doccula, F. G., Alfieri, A., Luoni, L., Ljung, K., Bonza, M. C., Stavrinidou, E., & Costa, A.
Current Biology. February 2023.
![Long-distance turgor pressure changes induce local activation of plant glutamate receptor-like channels [link]](//bibbase.org/img/filetypes/link.svg "Paper")
Paper
doi
link
bibtex
abstract
@article{grenzi_long-distance_2023,
title = {Long-distance turgor pressure changes induce local activation of plant glutamate receptor-like channels},
issn = {0960-9822},
url = {https://www.sciencedirect.com/science/article/pii/S0960982223000763},
doi = {10.1016/j.cub.2023.01.042},
abstract = {In Arabidopsis thaliana, local wounding and herbivore feeding provoke leaf-to-leaf propagating Ca2+ waves that are dependent on the activity of members of the glutamate receptor-like channels (GLRs). In systemic tissues, GLRs are needed to sustain the synthesis of jasmonic acid (JA) with the subsequent activation of JA-dependent signaling response required for the plant acclimation to the perceived stress. Even though the role of GLRs is well established, the mechanism through which they are activated remains unclear. Here, we report that in vivo, the amino-acid-dependent activation of the AtGLR3.3 channel and systemic responses require a functional ligand-binding domain. By combining imaging and genetics, we show that leaf mechanical injury, such as wounds and burns, as well as hypo-osmotic stress in root cells, induces the systemic apoplastic increase of L-glutamate (L-Glu), which is largely independent of AtGLR3.3 that is instead required for systemic cytosolic Ca2+ elevation. Moreover, by using a bioelectronic approach, we show that the local release of minute concentrations of L-Glu in the leaf lamina fails to induce any long-distance Ca2+ waves.},
language = {en},
urldate = {2023-03-23},
journal = {Current Biology},
author = {Grenzi, Matteo and Buratti, Stefano and Parmagnani, Ambra Selene and Abdel Aziz, Ilaria and Bernacka-Wojcik, Iwona and Resentini, Francesca and Šimura, Jan and Doccula, Fabrizio Gandolfo and Alfieri, Andrea and Luoni, Laura and Ljung, Karin and Bonza, Maria Cristina and Stavrinidou, Eleni and Costa, Alex},
month = feb,
year = {2023},
keywords = {glutamate receptor-like channels, implantable bioelectronic device, ligand-binding domain, long-distance Ca signaling},
}
In Arabidopsis thaliana, local wounding and herbivore feeding provoke leaf-to-leaf propagating Ca2+ waves that are dependent on the activity of members of the glutamate receptor-like channels (GLRs). In systemic tissues, GLRs are needed to sustain the synthesis of jasmonic acid (JA) with the subsequent activation of JA-dependent signaling response required for the plant acclimation to the perceived stress. Even though the role of GLRs is well established, the mechanism through which they are activated remains unclear. Here, we report that in vivo, the amino-acid-dependent activation of the AtGLR3.3 channel and systemic responses require a functional ligand-binding domain. By combining imaging and genetics, we show that leaf mechanical injury, such as wounds and burns, as well as hypo-osmotic stress in root cells, induces the systemic apoplastic increase of L-glutamate (L-Glu), which is largely independent of AtGLR3.3 that is instead required for systemic cytosolic Ca2+ elevation. Moreover, by using a bioelectronic approach, we show that the local release of minute concentrations of L-Glu in the leaf lamina fails to induce any long-distance Ca2+ waves.
Flexible Organic Electronic Ion Pump for Flow-Free Phytohormone Delivery into Vasculature of Intact Plants.
Bernacka-Wojcik, I., Talide, L., Abdel Aziz, I., Simura, J., Oikonomou, V. K., Rossi, S., Mohammadi, M., Dar, A. M., Seitanidou, M., Berggren, M., Simon, D. T., Tybrandt, K., Jonsson, M. P., Ljung, K., Niittylä, T., & Stavrinidou, E.
Advanced Science, n/a(n/a): 2206409. March 2023.
_eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1002/advs.202206409
Paper
doi
link
bibtex
abstract
@article{bernacka-wojcik_flexible_2023,
title = {Flexible {Organic} {Electronic} {Ion} {Pump} for {Flow}-{Free} {Phytohormone} {Delivery} into {Vasculature} of {Intact} {Plants}},
volume = {n/a},
issn = {2198-3844},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202206409},
doi = {10.1002/advs.202206409},
abstract = {Plant vasculature transports molecules that play a crucial role in plant signaling including systemic responses and acclimation to diverse environmental conditions. Targeted controlled delivery of molecules to the vascular tissue can be a biomimetic way to induce long distance responses, providing a new tool for the fundamental studies and engineering of stress-tolerant plants. Here, a flexible organic electronic ion pump, an electrophoretic delivery device, for controlled delivery of phytohormones directly in plant vascular tissue is developed. The c-OEIP is based on polyimide-coated glass capillaries that significantly enhance the mechanical robustness of these microscale devices while being minimally disruptive for the plant. The polyelectrolyte channel is based on low-cost and commercially available precursors that can be photocured with blue light, establishing much cheaper and safer system than the state-of-the-art. To trigger OEIP-induced plant response, the phytohormone abscisic acid (ABA) in the petiole of intact Arabidopsis plants is delivered. ABA is one of the main phytohormones involved in plant stress responses and induces stomata closure under drought conditions to reduce water loss and prevent wilting. The OEIP-mediated ABA delivery triggered fast and long-lasting stomata closure far away from the delivery point demonstrating systemic vascular transport of the delivered ABA, verified delivering deuterium-labeled ABA.},
language = {en},
number = {n/a},
urldate = {2023-03-21},
journal = {Advanced Science},
author = {Bernacka-Wojcik, Iwona and Talide, Loïc and Abdel Aziz, Ilaria and Simura, Jan and Oikonomou, Vasileios K. and Rossi, Stefano and Mohammadi, Mohsen and Dar, Abdul Manan and Seitanidou, Maria and Berggren, Magnus and Simon, Daniel T. and Tybrandt, Klas and Jonsson, Magnus P. and Ljung, Karin and Niittylä, Totte and Stavrinidou, Eleni},
month = mar,
year = {2023},
note = {\_eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1002/advs.202206409},
keywords = {bioelectronic devices, drug delivery, photo-crosslinking, plants vasculature, polyelectrolytes},
pages = {2206409},
}
Plant vasculature transports molecules that play a crucial role in plant signaling including systemic responses and acclimation to diverse environmental conditions. Targeted controlled delivery of molecules to the vascular tissue can be a biomimetic way to induce long distance responses, providing a new tool for the fundamental studies and engineering of stress-tolerant plants. Here, a flexible organic electronic ion pump, an electrophoretic delivery device, for controlled delivery of phytohormones directly in plant vascular tissue is developed. The c-OEIP is based on polyimide-coated glass capillaries that significantly enhance the mechanical robustness of these microscale devices while being minimally disruptive for the plant. The polyelectrolyte channel is based on low-cost and commercially available precursors that can be photocured with blue light, establishing much cheaper and safer system than the state-of-the-art. To trigger OEIP-induced plant response, the phytohormone abscisic acid (ABA) in the petiole of intact Arabidopsis plants is delivered. ABA is one of the main phytohormones involved in plant stress responses and induces stomata closure under drought conditions to reduce water loss and prevent wilting. The OEIP-mediated ABA delivery triggered fast and long-lasting stomata closure far away from the delivery point demonstrating systemic vascular transport of the delivered ABA, verified delivering deuterium-labeled ABA.
Chitosan-Modified Polyethyleneimine Nanoparticles for Enhancing the Carboxylation Reaction and Plants’ CO2 Uptake.
Routier, C., Vallan, L., Daguerre, Y., Juvany, M., Istif, E., Mantione, D., Brochon, C., Hadziioannou, G., Strand, Å., Näsholm, T., Cloutet, E., Pavlopoulou, E., & Stavrinidou, E.
ACS Nano, 17(4): 3430–3441. February 2023.
Publisher: American Chemical Society
Paper
doi
link
bibtex
abstract
@article{routier_chitosan-modified_2023,
title = {Chitosan-{Modified} {Polyethyleneimine} {Nanoparticles} for {Enhancing} the {Carboxylation} {Reaction} and {Plants}’ {CO2} {Uptake}},
volume = {17},
issn = {1936-0851},
url = {https://doi.org/10.1021/acsnano.2c09255},
doi = {10.1021/acsnano.2c09255},
abstract = {Increasing plants’ photosynthetic efficiency is a major challenge that must be addressed in order to cover the food demands of the growing population in the changing climate. Photosynthesis is greatly limited at the initial carboxylation reaction, where CO2 is converted to the organic acid 3-PGA, catalyzed by the RuBisCO enzyme. RuBisCO has poor affinity for CO2, but also the CO2 concentration at the RuBisCO site is limited by the diffusion of atmospheric CO2 through the various leaf compartments to the reaction site. Beyond genetic engineering, nanotechnology can offer a materials-based approach for enhancing photosynthesis, and yet, it has mostly been explored for the light-dependent reactions. In this work, we developed polyethyleneimine-based nanoparticles for enhancing the carboxylation reaction. We demonstrate that the nanoparticles can capture CO2 in the form of bicarbonate and increase the CO2 that reacts with the RuBisCO enzyme, enhancing the 3-PGA production in in vitro assays by 20\%. The nanoparticles can be introduced to the plant via leaf infiltration and, because of the functionalization with chitosan oligomers, they do not induce any toxic effect to the plant. In the leaves, the nanoparticles localize in the apoplastic space but also spontaneously reach the chloroplasts where photosynthetic activity takes place. Their CO2 loading-dependent fluorescence verifies that, in vivo, they maintain their ability to capture CO2 and can be therefore reloaded with atmospheric CO2 while in planta. Our results contribute to the development of a nanomaterials-based CO2-concentrating mechanism in plants that can potentially increase photosynthetic efficiency and overall plants’ CO2 storage.},
number = {4},
urldate = {2023-03-10},
journal = {ACS Nano},
author = {Routier, Cyril and Vallan, Lorenzo and Daguerre, Yohann and Juvany, Marta and Istif, Emin and Mantione, Daniele and Brochon, Cyril and Hadziioannou, Georges and Strand, Åsa and Näsholm, Torgny and Cloutet, Eric and Pavlopoulou, Eleni and Stavrinidou, Eleni},
month = feb,
year = {2023},
note = {Publisher: American Chemical Society},
pages = {3430--3441},
}
Increasing plants’ photosynthetic efficiency is a major challenge that must be addressed in order to cover the food demands of the growing population in the changing climate. Photosynthesis is greatly limited at the initial carboxylation reaction, where CO2 is converted to the organic acid 3-PGA, catalyzed by the RuBisCO enzyme. RuBisCO has poor affinity for CO2, but also the CO2 concentration at the RuBisCO site is limited by the diffusion of atmospheric CO2 through the various leaf compartments to the reaction site. Beyond genetic engineering, nanotechnology can offer a materials-based approach for enhancing photosynthesis, and yet, it has mostly been explored for the light-dependent reactions. In this work, we developed polyethyleneimine-based nanoparticles for enhancing the carboxylation reaction. We demonstrate that the nanoparticles can capture CO2 in the form of bicarbonate and increase the CO2 that reacts with the RuBisCO enzyme, enhancing the 3-PGA production in in vitro assays by 20%. The nanoparticles can be introduced to the plant via leaf infiltration and, because of the functionalization with chitosan oligomers, they do not induce any toxic effect to the plant. In the leaves, the nanoparticles localize in the apoplastic space but also spontaneously reach the chloroplasts where photosynthetic activity takes place. Their CO2 loading-dependent fluorescence verifies that, in vivo, they maintain their ability to capture CO2 and can be therefore reloaded with atmospheric CO2 while in planta. Our results contribute to the development of a nanomaterials-based CO2-concentrating mechanism in plants that can potentially increase photosynthetic efficiency and overall plants’ CO2 storage.
Combining scientific and local knowledge improves evaluating future scenarios of forest ecosystem services.
Hallberg-Sramek, I., Nordström, E., Priebe, J., Reimerson, E., Mårald, E., & Nordin, A.
Ecosystem Services, 60: 101512. April 2023.
Paper
doi
link
bibtex
abstract
@article{hallberg-sramek_combining_2023,
title = {Combining scientific and local knowledge improves evaluating future scenarios of forest ecosystem services},
volume = {60},
issn = {2212-0416},
url = {https://www.sciencedirect.com/science/article/pii/S2212041623000049},
doi = {10.1016/j.ecoser.2023.101512},
abstract = {Forest scenario analysis can help tackle sustainability issues by generating insight into the potential long-term effects of present-day management. In northern Sweden, forests provide important benefits including climate change mitigation, biodiversity conservation, reindeer husbandry, local livelihoods, and recreation. Informed by local stakeholders’ views on how forests can be enabled to deliver these benefits, we created four forest management scenarios: the close-to-nature scenario (CTN) which emphasises biodiversity conservation, the classic management scenario (CLA) optimising the forests’ net present value, the intensified scenario (INT) maximising harvested wood from the forest, and the combined scenario (COM) applying a combination of measures from the CTN and INT. The scenarios were applied to the local forest landscape and modelled over a 100-year simulation period, and the results of the modelling were then evaluated by a diverse group of stakeholders. For most ecosystem services, there was a time lag of 10–50 years before noticeable effects and differences between the scenarios became evident, highlighting the need to consider both the short- and long-term effects of forest management. Evaluation by the stakeholders put the modelled results into a local context. They raised considerations relating to wildlife and hunting, climate change risks, social acceptability, and conflict, highlighting the value of evaluating the scenarios qualitatively as well as quantitatively. Overall, stakeholders thought that the CTN and CLA scenarios promoted more ecosystem services and posed fewer climate risks, while also creating less conflict among stakeholders. Our results emphasise the value of combining scientific and local knowledge when developing and evaluating future forest scenarios.},
language = {en},
urldate = {2023-03-10},
journal = {Ecosystem Services},
author = {Hallberg-Sramek, Isabella and Nordström, Eva-Maria and Priebe, Janina and Reimerson, Elsa and Mårald, Erland and Nordin, Annika},
month = apr,
year = {2023},
keywords = {Forest management, Indigenous and local knowledge, Inter- and transdisciplinary research, Knowledge co-production, Scenario modelling, Stakeholder participation},
pages = {101512},
}
Forest scenario analysis can help tackle sustainability issues by generating insight into the potential long-term effects of present-day management. In northern Sweden, forests provide important benefits including climate change mitigation, biodiversity conservation, reindeer husbandry, local livelihoods, and recreation. Informed by local stakeholders’ views on how forests can be enabled to deliver these benefits, we created four forest management scenarios: the close-to-nature scenario (CTN) which emphasises biodiversity conservation, the classic management scenario (CLA) optimising the forests’ net present value, the intensified scenario (INT) maximising harvested wood from the forest, and the combined scenario (COM) applying a combination of measures from the CTN and INT. The scenarios were applied to the local forest landscape and modelled over a 100-year simulation period, and the results of the modelling were then evaluated by a diverse group of stakeholders. For most ecosystem services, there was a time lag of 10–50 years before noticeable effects and differences between the scenarios became evident, highlighting the need to consider both the short- and long-term effects of forest management. Evaluation by the stakeholders put the modelled results into a local context. They raised considerations relating to wildlife and hunting, climate change risks, social acceptability, and conflict, highlighting the value of evaluating the scenarios qualitatively as well as quantitatively. Overall, stakeholders thought that the CTN and CLA scenarios promoted more ecosystem services and posed fewer climate risks, while also creating less conflict among stakeholders. Our results emphasise the value of combining scientific and local knowledge when developing and evaluating future forest scenarios.
![Long-distance turgor pressure changes induce local activation of plant glutamate receptor-like channels [link]](http://bibbase.org/img/filetypes/link.svg "Paper")