Frankia [NiFe] uptake hydrogenases and genome reduction: different lineages of loss.
Pawlowski, K., Wibberg, D., Mehrabi, S., Obaid, N. B., Patyi, A., Berckx, F., Nguyen, H., Hagen, M., Lundin, D., Brachmann, A., Blom, J., Herrera-Belaroussi, A., Abrouk, D., Pujic, P., Hahlin, A., Kalinowski, J., Normand, P., & Sellstedt, A.
FEMS Microbiology Ecology,fiae147. October 2024.
Paper
doi
link
bibtex
abstract
@article{pawlowski_frankia_2024,
title = {Frankia [{NiFe}] uptake hydrogenases and genome reduction: different lineages of loss},
issn = {0168-6496},
shorttitle = {Frankia [{NiFe}] uptake hydrogenases and genome reduction},
url = {https://doi.org/10.1093/femsec/fiae147},
doi = {10.1093/femsec/fiae147},
abstract = {Uptake hydrogenase (Hup) recycles H2 formed by nitrogenase during nitrogen fixation, thereby preserving energy. Among root nodule bacteria, most rhizobial strains examined are Hup−, while only one Hup− Frankia inoculum had been identified. Previous analyses had led to the identification of two different [NiFe] hydrogenase syntons. We analysed the distribution of different types of [NiFe] hydrogenase in the genomes of different Frankia species. Our results show that Frankia strains can contain four different [NiFe] hydrogenase syntons representing groups 1f, 1h, 2a and 3b according to Søndergaard et al. (2016); no more than three types were found in any individual genome. The phylogeny of the structural proteins of groups 1f, 1h and 2a follows Frankia phylogeny; the phylogeny of the accessory proteins does not consistently. An analysis of different [NiFe] hydrogenase types in Actinomycetia shows that under the most parsimonious assumption, all four types were present in the ancestral Frankia strain. Based on Hup activities analysed and the losses of syntons in different lineages of genome reduction, we can conclude that groups 1f and 2a are involved in recycling H2 formed by nitrogenase while group 1h and group 3b are not.},
urldate = {2024-11-01},
journal = {FEMS Microbiology Ecology},
author = {Pawlowski, Katharina and Wibberg, Daniel and Mehrabi, Sara and Obaid, Nadia Binte and Patyi, András and Berckx, Fede and Nguyen, Han and Hagen, Michelle and Lundin, Daniel and Brachmann, Andreas and Blom, Jochen and Herrera-Belaroussi, Aude and Abrouk, Danis and Pujic, Petar and Hahlin, Ann-Sofi and Kalinowski, Jörn and Normand, Philippe and Sellstedt, Anita},
month = oct,
year = {2024},
pages = {fiae147},
}
Uptake hydrogenase (Hup) recycles H2 formed by nitrogenase during nitrogen fixation, thereby preserving energy. Among root nodule bacteria, most rhizobial strains examined are Hup−, while only one Hup− Frankia inoculum had been identified. Previous analyses had led to the identification of two different [NiFe] hydrogenase syntons. We analysed the distribution of different types of [NiFe] hydrogenase in the genomes of different Frankia species. Our results show that Frankia strains can contain four different [NiFe] hydrogenase syntons representing groups 1f, 1h, 2a and 3b according to Søndergaard et al. (2016); no more than three types were found in any individual genome. The phylogeny of the structural proteins of groups 1f, 1h and 2a follows Frankia phylogeny; the phylogeny of the accessory proteins does not consistently. An analysis of different [NiFe] hydrogenase types in Actinomycetia shows that under the most parsimonious assumption, all four types were present in the ancestral Frankia strain. Based on Hup activities analysed and the losses of syntons in different lineages of genome reduction, we can conclude that groups 1f and 2a are involved in recycling H2 formed by nitrogenase while group 1h and group 3b are not.
The Arabidopsis splicing factor PORCUPINE/SmE1 orchestrates temperature-dependent root development via auxin homeostasis maintenance.
El Arbi, N., Nardeli, S. M., Šimura, J., Ljung, K., & Schmid, M.
New Phytologist, 244(4): 1408–1421. 2024.
_eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1111/nph.20153
Paper
doi
link
bibtex
abstract
@article{el_arbi_arabidopsis_2024,
title = {The {Arabidopsis} splicing factor {PORCUPINE}/{SmE1} orchestrates temperature-dependent root development via auxin homeostasis maintenance},
volume = {244},
copyright = {© 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation.},
issn = {1469-8137},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/nph.20153},
doi = {10.1111/nph.20153},
abstract = {Appropriate abiotic stress response is pivotal for plant survival and makes use of multiple signaling molecules and phytohormones to achieve specific and fast molecular adjustments. A multitude of studies has highlighted the role of alternative splicing in response to abiotic stress, including temperature, emphasizing the role of transcriptional regulation for stress response. Here we investigated the role of the core-splicing factor PORCUPINE (PCP) on temperature-dependent root development. We used marker lines and transcriptomic analyses to study the expression profiles of meristematic regulators and mitotic markers, and chemical treatments, as well as root hormone profiling to assess the effect of auxin signaling. The loss of PCP significantly alters RAM architecture in a temperature-dependent manner. Our results indicate that PCP modulates the expression of central meristematic regulators and is required to maintain appropriate levels of auxin in the RAM. We conclude that alternative pre-mRNA splicing is sensitive to moderate temperature fluctuations and contributes to root meristem maintenance, possibly through the regulation of phytohormone homeostasis and meristematic activity.},
language = {en},
number = {4},
urldate = {2024-10-25},
journal = {New Phytologist},
author = {El Arbi, Nabila and Nardeli, Sarah Muniz and Šimura, Jan and Ljung, Karin and Schmid, Markus},
year = {2024},
note = {\_eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1111/nph.20153},
keywords = {Arabidopsis thaliana, SmE, alternative RNA splicing, auxin signaling, root apical meristem, root development, temperature signaling},
pages = {1408--1421},
}
Appropriate abiotic stress response is pivotal for plant survival and makes use of multiple signaling molecules and phytohormones to achieve specific and fast molecular adjustments. A multitude of studies has highlighted the role of alternative splicing in response to abiotic stress, including temperature, emphasizing the role of transcriptional regulation for stress response. Here we investigated the role of the core-splicing factor PORCUPINE (PCP) on temperature-dependent root development. We used marker lines and transcriptomic analyses to study the expression profiles of meristematic regulators and mitotic markers, and chemical treatments, as well as root hormone profiling to assess the effect of auxin signaling. The loss of PCP significantly alters RAM architecture in a temperature-dependent manner. Our results indicate that PCP modulates the expression of central meristematic regulators and is required to maintain appropriate levels of auxin in the RAM. We conclude that alternative pre-mRNA splicing is sensitive to moderate temperature fluctuations and contributes to root meristem maintenance, possibly through the regulation of phytohormone homeostasis and meristematic activity.
Molecular advances in bud dormancy in trees.
Ding, J., Wang, K., Pandey, S., Perales, M., Allona, I., Khan, M. R. I., Busov, V. B, & Bhalerao, R. P
Journal of Experimental Botany, 75(19): 6063–6075. October 2024.
Paper
doi
link
bibtex
abstract
@article{ding_molecular_2024,
title = {Molecular advances in bud dormancy in trees},
volume = {75},
issn = {0022-0957},
url = {https://doi.org/10.1093/jxb/erae183},
doi = {10.1093/jxb/erae183},
abstract = {Seasonal bud dormancy in perennial woody plants is a crucial and intricate process that is vital for the survival and development of plants. Over the past few decades, significant advancements have been made in understanding many features of bud dormancy, particularly in model species, where certain molecular mechanisms underlying this process have been elucidated. We provide an overview of recent molecular progress in understanding bud dormancy in trees, with a specific emphasis on the integration of common signaling and molecular mechanisms identified across different tree species. Additionally, we address some challenges that have emerged from our current understanding of bud dormancy and offer insights for future studies.},
number = {19},
urldate = {2024-10-18},
journal = {Journal of Experimental Botany},
author = {Ding, Jihua and Wang, Kejing and Pandey, Shashank and Perales, Mariano and Allona, Isabel and Khan, Md Rezaul Islam and Busov, Victor B and Bhalerao, Rishikesh P},
month = oct,
year = {2024},
pages = {6063--6075},
}
Seasonal bud dormancy in perennial woody plants is a crucial and intricate process that is vital for the survival and development of plants. Over the past few decades, significant advancements have been made in understanding many features of bud dormancy, particularly in model species, where certain molecular mechanisms underlying this process have been elucidated. We provide an overview of recent molecular progress in understanding bud dormancy in trees, with a specific emphasis on the integration of common signaling and molecular mechanisms identified across different tree species. Additionally, we address some challenges that have emerged from our current understanding of bud dormancy and offer insights for future studies.
Exploring the world of small proteins in plant biology and bioengineering.
Petri, L., Van Humbeeck, A., Niu, H., Ter Waarbeek, C., Edwards, A., Chiurazzi, M. J., Vittozzi, Y., & Wenkel, S.
Trends in Genetics. October 2024.
Paper
doi
link
bibtex
abstract
@article{petri_exploring_2024,
title = {Exploring the world of small proteins in plant biology and bioengineering},
issn = {0168-9525},
url = {https://www.sciencedirect.com/science/article/pii/S0168952524002129},
doi = {10.1016/j.tig.2024.09.004},
abstract = {Small proteins are ubiquitous in all kingdoms of life. MicroProteins, initially characterized as small proteins with protein interaction domains that enable them to interact with larger multidomain proteins, frequently modulate the function of these proteins. The study of these small proteins has contributed to a greater comprehension of protein regulation. In addition to sequence homology, sequence-divergent small proteins have the potential to function as microProtein mimics, binding to structurally related proteins. Moreover, a multitude of other small proteins encoded by short open reading frames (sORFs) and peptides, derived from diverse sources such as long noncoding RNAs (lncRNAs) and miRNAs, contribute to a variety of biological processes. The potential of small proteins is evident, offering promising avenues for bioengineering that could revolutionize crop performance and reduce reliance on agrochemicals in future agriculture.},
urldate = {2024-10-18},
journal = {Trends in Genetics},
author = {Petri, Louise and Van Humbeeck, Anne and Niu, Huanying and Ter Waarbeek, Casper and Edwards, Ashleigh and Chiurazzi, Maurizio Junior and Vittozzi, Ylenia and Wenkel, Stephan},
month = oct,
year = {2024},
keywords = {lncRNA, microProteins, sORFs, transcription factor},
}
Small proteins are ubiquitous in all kingdoms of life. MicroProteins, initially characterized as small proteins with protein interaction domains that enable them to interact with larger multidomain proteins, frequently modulate the function of these proteins. The study of these small proteins has contributed to a greater comprehension of protein regulation. In addition to sequence homology, sequence-divergent small proteins have the potential to function as microProtein mimics, binding to structurally related proteins. Moreover, a multitude of other small proteins encoded by short open reading frames (sORFs) and peptides, derived from diverse sources such as long noncoding RNAs (lncRNAs) and miRNAs, contribute to a variety of biological processes. The potential of small proteins is evident, offering promising avenues for bioengineering that could revolutionize crop performance and reduce reliance on agrochemicals in future agriculture.