Microscopy Facility

All users are reminded to acknowledge in publications, presentations and posters on the role of the facilities and expertise. 
"The authors acknowledge the facilities and technical assistance of the Umeå Plant Science Centre (UPSC) Microscopy Facility”

The microscopy facilities at UPSC include a broad range of equipment for sample preparation, subsequent analysis and image acquisition. We work with different sample sizes, starting from thick sections (hundret of μm) semi thin sections (20 to 0.5 um) and even with ultrathin sections or nanoresolution (AFM). Users of the microscopy facilities can perform their experiments after appropriate introduction to the different instruments. We are here to introduce you to fascinating micrsocopy woarld, to advise, to give a proper training according to your needs and to advice during experiental procedures.

Whole cross section of poplar stem from microscope, stained with safranin and alcianblue.Cross section of poplar stem, stained with safranin and alcianblue.Whole cross section of poplar stem from microscope, stained for starch.Cross section of poplar stem, stained for starch.

Available equipment:

1. Microscopes

  1. Two Zeiss Axioplan (fluorescence) microscopes with digital camera systems

  2.  THUNDER Fluorescence stereomicroscope Leica M205 with two digital cameras and motorize stage

  3. Epifluorescence microscope Leica DMi8 fully motorized with two digital cameras

  4. Fluorescence stereomicroscope Leica M205 FA with two digital cameras  and Leica AFL III

  5. Leica AFL III in dark room

2. Confocal laser scanning microscopes

  1. Zeiss LSM880 CLSM with airyscan, airyfast, FLIM, FCS, FCCS, inverted stand

  2. Zeiss LSM800 CLSM with airyscan, upright stand

  3. Leica Stellaris 8 DIVE, inverted stand

  4.  Zeiss LSM 980, inverted stand

  5. Nikon CrEST Cicero Spinning Disks, upright stand

  6. Image analysis computer with full version of Zeiss and Leica softwares

3. Atomic Force Microscope

  1. NanoWizard® 4 XP BioScience, BioScience with VortisTM2 Advanced​ version SPM Controller, HybridStageTM, DirectOverlayTM 2 software module and QITM- Advanced 2 software module

4. Sectioning devices

  1. Two Leica vibratomes VT1000S

  2. Zeiss HM 350 microtome for wax embedded material sectioning

  3. Cryostat - Cryo Star NX70 with CroJane tap system

  4. Glass Knife Maker

5. Laser capture microdissector (LCM) - The PALM® RoboMover

6.Automated in situ hybridization or immuno-histochemistry labelling

  1. Intavis InsituPro VSi automated system

7. Fluorescence Activated Cell Sorter (FACS)

  1. BD FACSAria Flow Cytometer

  2. Melody FACS

For more information of the equipment see respective description in the menu to the right. For introductions or questions please contact concerned contact person.

Contact:

Stéphane Verger, Asisstant Professor
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Marta Derba-Maceluch, Staff scientist (AFM, light microscopy and sectioning)
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Ioanna Antoniadi, Staff scientist (FACS)
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Siamsa Doyle, Staff scientist (InsituPro VSi)
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