Laser capture microdissection (LCM) is an automated sample preparation technique which uses a focused laser to cut and a pulsed, defocused laser to "catapult" a specific tissue sample from a slide into a collection tube. It allows the identification of specific substructures or cell types and their precise removal from the original tissue without causing damage. This technic allows for the non-contact isolation of single cells or specific cell groups from a heterogeneous mixture, making it possible to downstream microgenomics applications such as analyse of DNA, RNA, and proteins from a pure and uncontaminated sample.
At UPSC Microscopy Facility we have Zeiss PALM system equipped in objectives 5x 10x 20x 40x 63x 100x and Pulsed Solid-Stat Laser 355 nm (UV-A) less harmful for DNA, RNA and protein and Live cells.
LCM is located at room KB.K6 (B6.22.49) on 6th floor. for introduction please contact 