UPSC has several microscopes where bright field, DIC, dark field, phase contrast and epifluorescence imaging can be performed. 

Microscopes available at UPSC:

Epifluorescence microscope Leica DMi8

The microscope is inverted, fully motorized with a scanning stageMicroscope Leica DMi8 at UPSC.Microscope Leica DMi8 at UPSC. and motorized focus to allow Z stack acquisition. There are seven objective lenses 5x/0.15, 10x/0.32, 20x/0.4 corr 0-2 mm DIC, 40x/0.6 corr 0-2 mm DIC, 40x/1.1 water corr DIC, 63x/1.3 glycerol corr DIC and 100x/1.32 oil DIC. The change between objectives is made with parafocality, automatic adjustment for Koehler illumination and contrasting method allowing full reproducibility between sessions. In addition, fluorescence imaging for DAPI, GFP and mCherry can be made with fast switching between brightfield/DIC and fluorescence. There are also conventional filter sets for DAPI LP and BP filter, CFP, GFP LP and BP filter, YFP, RFP, and Cy5 (only visible by camera) where the 16 individually selectable LED light sources (in the range of 365-770 nm) can be fully controlled by the software. There are holders for slides of different length, 3 cm round Petri dishes and multiwell plates. The system is equipped with a Leica DFC 7000 T color camera which is sensitive enough for fluorescence and can do imaging at 40 fps. In addition, there is an even more sensitive monochrome Leica DFC 9000GT sCMOS camera with improved signal-to-noise ratio compared to the color camera. The system can be fully controlled by the software to allow easy changes between objectives, different fluorescence/DIC/bright field settings, stage positions, focus in combination with time lapse. The system is also triggered for fast imaging possibilities.
It has LAS X navigator for easy imaging options where one can do preview overview imaging of the whole sample. This overview can then be used to select one or more interesting regions for further imaging at high resolution where tile and stitching will be automatically recognized by the software when the sample is too large to fit in one image with the selected objective. There are autofocus algorithms or manual possibility to keep focus along a sample for tile imaging, which makes it possible to “straighten out” an unevenly mounted sample. In addition, the software can do 2D deconvolution of fluorescence images or extended depth of focus for color images.
Applications: Virtually any microscopy work that can be done on an inverted stage, which does not need phase contrast or dark field.
This microscope is located in room KB.K6 (B6.22.49) This instrument is operated on an hourly fee basis. You are allowed to use this microscope only after passing a mandatory introduction! A basic introduction to this instrument usually takes 2 h. Due to heavy usage, expect to wait 3 weeks or more for an introduction. For introduction or other questions regarding this instrument, please contact Marta Derba-Maceluch.

Epifluorescence microscope Zeiss Axioplan 2

Poplar stem section stained for lignin detection (Mule staining).Poplar stem section stained for lignin detection (Mule staining).Axioplan 2 microscope at UPSC.Axioplan 2 microscope at UPSC.

The microscope is upright, manual, equipped with colour CCD camera and a range of objectives (4x-100x). Microscope is equiped in conventional filter sets DAPI, GFP/YFP LP, RFP LP, GFP, CFP and CFP+GFP.  MIcroscope is eqiped in Lumencor SOLA SM II Solid State White Light Engine LED lamp.

This microscope is located in room KB.K6 (B6.24.51) This instrument is operated on an hourly fee basis. You are allowed to use this microscope only after passing a mandatory introduction! For introduction or other questions regarding this instrument, please contact Marta Derba-Maceluch.

Epifluorescence microscope Zeiss AxioObserver A1m

Zeiss AxioObserver A1m at UPSC.Zeiss AxioObserver A1m at UPSC.Fragment of poplar stem, cross section with visible tension wood. Fragment of poplar stem, cross section with visible tension wood.

The microscope is inverted, manual, it has equipped with colour CCD camera and a range of objectives (4x-100x) with 2 of then as long working distans objective perfect for observation of bigger objects like callus. There are holders for slides of different length, 3 cm round Petri dishes and multiwell plates.  Microscop is equipped with standard mercury bulb. This instrument is operated on an hourly fee basis. You are allowed to use this microscope only after passing a mandatory introduction! For introduction or other questions regarding this instrument, please contact Marta Derba-Maceluch.

Other available microscopes:
Zeiss AxioStar upright epifluorescence microscope equipped with standard mercury lamp and 10x-40x objectives is located in room KB.K6 (B6.20.51).
Zeiss Axioplan upright microscope equipped with colour CCD camera and computer is available to borrow from facility.
Axiovert 40 CFL inverted long working distance inverted epifluorescence microscope with holders for Petri dishes and multiwell plates equipped with standard mercury lamp and monochromatic CCD camera. located in room KB.K6 (B6.18.51).
Zeiss Standard 25 small upright microscope with two pair oculas for simuntaniosly observation by two persons.  located in room KB.K6 (B6.18.51). 
Whole cross section of poplar stem from microscope, stained with safranin and alcianblue.

Whole cross section of poplar stem from microscope, stained with safranin and alcianblue.Whole cross section of poplar stem from microscope, stained for starch.Whole cross section of poplar stem from microscope, stained for starch.

Stereomicroscopes available at UPSC:

Leica M205 FA stereomicroscope with Fluorescence

Fully motorized system with parafocal objectives of 0.63x Plan-Apo Epifluorescent strereomicroscope  Leica M205FA at UPSC.Epifluorescent strereomicroscope Leica M205FA at UPSC.with a working distance of 67 mm and 2x/0.35 Plan-Apo with a working distance of 20.1 mm and a 0-5 mm correction ring for water pillar (or agar). The objectives are mounted on a revolving nosepiece and can achieve 4.7 - 320x zoom magnification range that corresponds to a work field diameter of 46.1 – 0.72 mm. It is mounted with a high precision scanning stage that can be manually operated or motor controlled without losing linearity.
It has episcopic light via gooseneck LED lamps and transmitted light via a light base that is equipped with motorized Rottermann contrast TM, brightfield and two-sided darkfield. Fluorescence by LED light source and bandpass filter sets for DAPI, CFP, GFP, YFP and RFP, in addition to long pass filter set for GFP. A DMC6200 digital pixel shift color camera is used for image acquisition. In addition, it is also equipped with a sensitive monochrome Leica DFC 9000GT sCMOS camera for improved fluorescence imaging. The stereomicroscope has ergonomic setup of ocular, tube and light base.
With this system one can do imaging of brightfield and/or fluorescence multichannel acquisition, time lapse images, 3D/Z stacks, overview images of large field of views, tile scan acquisition and stitching, spiral scan acquisition, 2D deconvolution and it has several analysis tools. 
Applications: fast screening of fluorescence zygosity on plates with fast overview imaging or ocularly, GUS overview images and then more detailed imaging over large areas using tiles and stitching etc.
The stereomicroscope is located in the room inside KB.K2 (B2.18.51). This instrument is operated on an hourly fee basis. You are allowed to use this stereomicroscope only after passing a mandatory introduction! A basic introduction to this instrument usually takes 2 h. Due to heavy usage, expect to wait 3 weeks or more for an introduction. For introduction or other questions regarding this instrument, please contact Marta Derba-Maceluch or Judith Felten.

Leica MZFL III epifluorescence stereomicroscope
(8-100x magnification) is available for fast screening of fluorescence (filter sets for CFP, GFP, YFP, and RFP). This microscope is located in room KB.K6 (B6.22.49). Temporary in room B3-42-45
Arabidopsis stem cross section with visible vascular bundle.Arabidopsis stem cross section with visible vascular bundle. Stained with TBO.Fluorescencent stereomicroscope Leica AFL III at UPSC.Fluorescencent stereomicroscope Leica AFL III at UPSC.


















In addition:.

  • 3 stereomicroscopes are located in greenhouse, one with CCD camera.
  • stereomicroscope located in room KB.K6 (B6.44.51) equiped in CCD camera.
  • 4 regular stereomicroscopes epi-illuminated, two of which are equipped as well with bottom illumination.