| No | Tasks, Milestones | Year 1 Month |
Year 2 Month |
Year 3 Month |
Year 4 Month |
Year 5 Month |
| 1-1 | Metabolic bottlenecks identified in the most promising transgenic lines using carbon-13 flux measurements. Strategy designed to overcome the bottlenecks. |
30 | ||||
| 1-2 | WRI1 and LEC1 overexpressors produced and oil content analyzed | 18 | ||||
| 1-3 | Polycomb targets involved in oil accumulation identified | 12 | ||||
| 1-4 | Polycomb targets for increasing oil accumulation tested in transgenic hybrid aspen | 36 | ||||
| 1-5 | AGPase downregulated plants generated and analyzed for oil | 24 | ||||
| 1-6 | Genetic loci and markers underlying oil accumulation in trees identified | 48 | ||||
| 2-1 | Strategies of xylan deacetylation for bioconversion evaluated based on field experiments | 48 | ||||
| 2-2 | Mechanism of stress responses elucidated and strategies for xylan side chain manipulation designed | 36 | ||||
| 2-3 | Stability of xylan backbone manipulation effects on growth and bioconversion evaluated based on field experiments | 48 | ||||
| 3-1 | Transgenic trees with strong modifications in lignin content of the wood | 12 | ||||
| 3-2 | Transgenic trees with modified lignin polymer size | 24 | ||||
| 3-3 | Transgenic trees with modified lignin composition | 24 | ||||
| 3-4 | Trees superior for biochemical conversion | 36 | ||||
| 3-5 | Strategies to increase carbon flow to lignocellulose | 48 | ||||
| 3-6 | Improved genotypes tested in the field conditions | 60 |
Milestones
The milestones of the project are listed in a table below and the projected timing of deployment of the different deliverables of this project is shown in Figure 2.