Cutting Edge Seminar
Technische Universität München TUM, School of Life Sciences
Weihenstephan/Munich, GermanyTitle: PIN-ning down the function of D6PK protein kinases in auxin transport
Host: Markus Schmid
Place Lilla hörsalen, KB3A9Abstract:
The biosynthesis and proper distribution of the plant hormone auxin within the plant influences almost every aspect of plant growth and development. To the largest extent, differences in the morphology between plants are the results of differential auxin distribution and auxin actions at the level of individual plants cells. Whereas auxin transport from cell to cell and within a plant had for a long time been inferred solely based on the polar distribution of PIN auxin exporters, we have recently shown that PINs require activation by protein kinases, such as the D6 PROTEIN KINASE, to become active auxin transporters. I will describe the biochemistry of D6PK and mechanisms that are required for targeting D6PK to the plasma membrane and the PINs. I will also present data that challenge the current view of how PIN polarity is controlled by phosphorylation. Finally, I will highlight some other regulatory functions of D6PK that are independent of PIN-dependent auxin transport control.
Arabidopsis D6PK is a lipid domain-dependent mediator of root epidermal planar polarity. Stanislas T et al.; Nature Plants 1, 15162 (2015)
Dynamic control of auxin transport-dependent growth by AGCVIII protein kinases. Barbosa IC and Schwechheimer C.; Curr Opin Plant Biol (2014) 22:108-15.
Auxin efflux by PIN-FORMED proteins is activated by two different protein kinases, D6 PROTEIN KINASE and PINOID. Zourelidou M et al. Elife (2014) 19;3.
D6 PROTEIN KINASE activates auxin transport-dependent growth and PIN-FORMED phosphorylation at the plasma membrane. Barbosa IC, Zourelidou M, Willige BC, Weller B, Schwechheimer C. Dev Cell (2014) 29(6):674-85.
D6PK AGCVIII kinases are required for auxin transport and phototropic hypocotyl bending in Arabidopsis. Willige BC et al. Plant Cell (2013) 25(5):1674-88
The polarly localized D6 PROTEIN KINASE is required for efficient auxin transport in Arabidopsis thaliana. Zourelidou M, Müller I, Willige BC, Nill C, Jikumaru Y, Li H, Schwechheimer C. Development. 2009 Feb;136(4):627-36.
In addition to the work on auxin transport that Claus will discuss about in his presentation, his lab has a long-staninding interest in the function of the COP9 signalosome and gibberellic acid (GA) signaling. More information can be found here: http://sysbiol.wzw.tum.de/index.php?id=2&L=1