Keech, Olivier - Stress-induced senescence and its subsequent metabolic regulations

Research

Olivier Keech sitting at the desk in his office

Photo: Fredrik Larsson

Our research explores several aspects of the regulation of plant metabolism in response to stress, with a particular emphasis on mitochondrial metabolism. In plants, the process of aging as well as many environmental constraints may lead to the death of leaves. This particular type of cell death is often referred to as leaf senescence and can have a profoundly negative impact on crop yields and post-harvest shelf-life.

Aim: Leaves are essential plant structures and their well-being is crucial for plant development and survival. When a stress is applied, a plant has two options: try to cope with it or induce senescence and reallocate valuable nutrients towards new, developing or storage organs. A mutual antagonistic relationship can summarize this phenomenon as shown in figure 1. Our aim is to understand how the plant validates senescence over an adaptation strategy in response to stress (Fig. 1). This work mainly covers two aspects: 1) to unveil the communication and signalling mechanisms controlling the induction of leaf senescence and 2) to determine the subsequent metabolic regulation that occurs in response to stress, and ultimately during leaf senescence.

Illustration about the relationship between adaptation and senscence including metabolic/redox balance, hormonal homeostasis, gene regulatory network and different types of stress

Figure 1: Mutual antagonistic relationship between adaptation and induction of senescence in response to a stress (e.g. nutrient deficiency, light regime, temperatures, pathogene infection, etc).

1. Using dark-induced senescence as a proxy to decipher signalling pathways controlling the induction of leaf senescence

In earlier studies (Keech et al., 2007; Law et al., 2018), we have shown that a leaf from a plant entirely darkened (DP) can survive much longer than an individually-darkened leaf (IDL; Fig. 2), which suggests that upon the right signals, the induction of leaf senescence can be repressed and alternative metabolic strategies conferring extended longevity can occur.

On the left side, an individual leaf was darkened and this leaf turned yellow after 6 days of treatment. On the right side, an entire plant was darkended and stayed mostly green after 6 days of treatment.

Figure 2: Experimental setup for the two darkening treatments (Weaver and Amasino, 2001; Keech et al., 2007; Law et al., 2018).

Yet, our current knowledge on the respective metabolic adjustments remains highly fragmented. In 2018, we proposed the following working models (Fig. 3).

Illustration summarising metabolic strategies in plant leaves to darkening

Comic strip by Neil E. Robbins II illustrating the effects of shading in plants

Figure 3: A) Model summarising the different metabolic strategies employed by plants in response to partial or total darkening of the plant. Size and line-weight of the fonts and arrows are proportional to their implication to these metabolic processes. The large arrow behind the leaf in DP conditions depicts the conserved metabolic strategy main-tained between 3 and 6 days of darkening. Abbreviations: AAA - aromatic amino acids, BCAA - branched chain amino acids, Citr - citrate, mETC - mitochondrial electron trans-port chain, OAA - oxaloacetate, PPP - pentose phosphate pathway, Shik/Chor - shikimate/chorismate, TCA - tricarboxylic acid cycle (Law et al., 2018); B) "Are plants afraid of the dark?" Comic strip by Neil E. Robbins II explaining the content of the publication in a humoristic way. Find the full comic strip here: https://neilercomics.com/2018/05/18/are-plants-afraid-of-the-dark/

However, in order to challenge these hypotheses, we are currently investigating the metabolic regulations in a set of functional stay-green mutants issued from a genetic screen. This provides us with a much valuable tool to determine how cells can survive prolonged stress conditions.

2. Regulation of metabolism during leaf senescence

In a green leaf, the three energy organelles (peroxisome, mitochondrion and chloroplast) work in synergy to sustain an efficient assimilation of carbon while constantly maintaining the essential functions of the cell. However, when a leaf undergoes senescence (“yellowing”), whole cell-metabolism is drastically modified, and as chloroplasts are rapidly getting impaired, the remaining organelles acquire novel functions, particularly the mitochondrion. In animals, mitochondria have been shown to integrate various signals and to subsequently modulate cell death processes whereas in plants, the contribution of mitochondria in cell death regulation remains unclear, particularly during leaf senescence.

Therefore, we are currently investigating in more detail the role of mitochondria during both developmental (i.e. aging) and stress-induced leaf senescence (Fig. 4). Illustration of metabolic processes in the mitochondrium Figure 4: Production of glutamate, reducing equivalents and TCA cycle intermediates from catabolic reactions occurring in the mitochondrion during developmental leaf senescence (Chrobok et al., 2016). Transcriptomic overview of the mitochondrially localised portion of the following metabolic pathways: (I) Lysine degradation, (II) branched chain amino acid degradation, (III) D-2HG metabolism, (IV) Glycine and Alanine metabolism, (V) Urea Cycle and (VI) Proline metabolism. Specific genes of these pathways and their transcript abundance during developmental leaf senescence are illustrated here. Production of reducing equivalents is shown as an arrow with an electron (e-).

3. Towards sustainable food production

Among a few other things, we are also interested in complementary alternatives for food production systems. In particular, we are involved in several projects aiming at developing integrated aqua-agro systems in closed land-based units. The strategic implementation of numerous trophic layers within a production system is a natural way to achieve a higher sustainability while maintaining the whole production economically viable.

A concept scheme (Fig. 5), released for the PLATSEN* event end of 2016 depicts some of the interrelationships between the different trophic layers that can be implemented to for example urban farming system in order to achieve a circularity, i.e. a better use of biowaste, energy and resources.

Depiction of the nutrient cycle between the different components of the eMTE model

Schematic overview of the eMulti-Trophic Ecosystem (eMTE) concept

The eMulti-Trophic Ecosystem – a potential model for sustainable food production from Umeå University on Vimeo. Interview with Olivier Keech at PLATSEN in 2016 about the eMTE project. More information about the eMTE project and the exhibition at PLATSEN in 2016.

PLATSEN is thought as a platform where decision makers, politicians, scientists, NGOs and people from public and private sectors can meet and exchange and discuss ideas about sustainability in an urban environment. The 2016 event was initiated by the Swedish Scientific Council for Sustainability in collaboration with several other actors from the public and private sectors e.g. Umeå Municipality and Umeå University.

Integrated fish and plant production workshop 2021

"Towards sustainable urban food production with multi-trophic systems", talk starts at 59 min: Link to the recorded workshop on SLU Play

Publications

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2022 (2)

Cell Type–Specific Isolation of Mitochondria in Arabidopsis. Boussardon, C., & Keech, O. In Van Aken, O., & Rasmusson, A. G., editor(s), Plant Mitochondria: Methods and Protocols, of Methods in Molecular Biology, pages 13–23. Springer US, New York, NY, January 2022.

Cell Type–Specific Isolation of Mitochondria in Arabidopsis [link]

Paper link bibtex abstract

@incollection{boussardon_cell_2022,
	address = {New York, NY},
	series = {Methods in {Molecular} {Biology}},
	title = {Cell {Type}–{Specific} {Isolation} of {Mitochondria} in {Arabidopsis}},
	isbn = {978-1-07-161653-6},
	url = {https://doi.org/10.1007/978-1-0716-1653-6_2},
	abstract = {Membrane-bound organelles are unique features of eukaryotic cell structures. Among them, mitochondria host key metabolic functions and pathways, including the aerobic respiration. In plants, several procedures are available to isolate mitochondria from the other cell compartments, as high-quality purified extracts are often necessary for accurate molecular biology or biochemistry investigations. Protocols based on differential centrifugations and subsequent density gradients are an effective way to extract rather pure and intact mitochondria within a few hours. However, while mitochondria from seedlings, large leaves or tubers are relatively easy to extract, tissue-specific isolation of organelles had remained a challenge. This has recently been circumvented, only in transformable plants though, by the use of affinity-tagged mitochondria and their isolation with magnetic beads.We hereby describe a step-by-step protocol for the rapid and tissue-specific isolation of Arabidopsis thaliana mitochondria, a method named IMTACT (Isolation of Mitochondria TAgged in specific Cell Types). Cell-specific biotinylated mitochondria are isolated with streptavidin magnetic beads in less than 30 min from sampling to final extract. Key steps, enrichment, bead size comparison, and mitochondrial depletion in the sample are also reported in order to facilitate the experimental setup of the user.},
	language = {en},
	urldate = {2021-09-23},
	booktitle = {Plant {Mitochondria}: {Methods} and {Protocols}},
	publisher = {Springer US},
	author = {Boussardon, Clément and Keech, Olivier},
	editor = {Van Aken, Olivier and Rasmusson, Allan G.},
	month = jan,
	year = {2022},
	keywords = {Biotin–streptavidin interaction, Editable Golden Gate plasmids, Mitochondria, Tagged outer membrane, Tissue-specific isolation},
	pages = {13--23},
}

Protein lipoylation in mitochondria requires Fe–S cluster assembly factors NFU4 and NFU5. Przybyla-Toscano, J., Maclean, A. E, Franceschetti, M., Liebsch, D., Vignols, F., Keech, O., Rouhier, N., & Balk, J. Plant Physiology, 188(2): 997–1013. February 2022.

Protein lipoylation in mitochondria requires Fe–S cluster assembly factors NFU4 and NFU5 [link]

Paper doi link bibtex abstract

@article{przybyla-toscano_protein_2022,
	title = {Protein lipoylation in mitochondria requires {Fe}–{S} cluster assembly factors {NFU4} and {NFU5}},
	volume = {188},
	issn = {0032-0889},
	url = {https://doi.org/10.1093/plphys/kiab501},
	doi = {10.1093/plphys/kiab501},
	abstract = {Plants have evolutionarily conserved NifU-like (NFU)-domain proteins that are targeted to plastids or mitochondria. ‘Plastid-type’ NFU1, NFU2 and NFU3 in Arabidopsis (Arabidopsis thaliana) play a role in iron-sulfur (Fe-S) cluster assembly in this organelle, whereas the type-II NFU4 and NFU5 proteins have not been subjected to mutant studies in any plant species to determine their biological role. Here, we confirmed that NFU4 and NFU5 are targeted to the mitochondria. The proteins were constitutively produced in all parts of the plant, suggesting a housekeeping function. Double nfu4 nfu5 knockout mutants were embryonic lethal, and depletion of NFU4 and NFU5 proteins led to growth arrest of young seedlings. Biochemical analyses revealed that NFU4 and NFU5 are required for lipoylation of the H proteins of the glycine decarboxylase complex and the E2 subunits of other mitochondrial dehydrogenases, with little impact on Fe-S cluster-containing respiratory complexes or aconitase. Consequently, the Gly-to-Ser ratio was increased in mutant seedlings and early growth improved with elevated CO2 treatment. In addition, pyruvate, 2-oxoglutarate and branched-chain amino acids accumulated in nfu4 nfu5 mutants, further supporting defects in the other three mitochondrial lipoate-dependent enzyme complexes. NFU4 and NFU5 interacted with mitochondrial lipoyl synthase (LIP1) in yeast 2-hybrid and bimolecular fluorescence complementation assays. These data indicate that NFU4 and NFU5 have a more specific function than previously thought, most likely providing Fe-S clusters to lipoyl synthase.},
	number = {2},
	urldate = {2021-11-04},
	journal = {Plant Physiology},
	author = {Przybyla-Toscano, Jonathan and Maclean, Andrew E and Franceschetti, Marina and Liebsch, Daniela and Vignols, Florence and Keech, Olivier and Rouhier, Nicolas and Balk, Janneke},
	month = feb,
	year = {2022},
	pages = {997--1013},
}

2021 (2)

Gene atlas of iron‐containing proteins in Arabidopsis thaliana. Przybyla‐Toscano, J., Boussardon, C., Law, S. R., Rouhier, N., & Keech, O. The Plant Journal, 106(1): 258–274. April 2021.

Gene atlas of iron‐containing proteins in Arabidopsis thaliana [link]

Paper doi link bibtex

@article{przybylatoscano_gene_2021,
	title = {Gene atlas of iron‐containing proteins in {Arabidopsis} thaliana},
	volume = {106},
	issn = {0960-7412, 1365-313X},
	url = {https://onlinelibrary.wiley.com/doi/10.1111/tpj.15154},
	doi = {10/gkcr7c},
	language = {en},
	number = {1},
	urldate = {2021-06-03},
	journal = {The Plant Journal},
	author = {Przybyla‐Toscano, Jonathan and Boussardon, Clément and Law, Simon R. and Rouhier, Nicolas and Keech, Olivier},
	month = apr,
	year = {2021},
	pages = {258--274},
}

Iron–sulfur proteins in plant mitochondria: roles and maturation. Przybyla-Toscano, J., Christ, L., Keech, O., & Rouhier, N. Journal of Experimental Botany, 72(6): 2014–2044. March 2021.

Iron–sulfur proteins in plant mitochondria: roles and maturation [link]

Paper doi link bibtex abstract

@article{przybyla-toscano_ironsulfur_2021,
	title = {Iron–sulfur proteins in plant mitochondria: roles and maturation},
	volume = {72},
	issn = {0022-0957, 1460-2431},
	shorttitle = {Iron–sulfur proteins in plant mitochondria},
	url = {https://academic.oup.com/jxb/article/72/6/2014/6029934},
	doi = {10.1093/jxb/eraa578},
	abstract = {Abstract
            Iron–sulfur (Fe–S) clusters are prosthetic groups ensuring electron transfer reactions, activating substrates for catalytic reactions, providing sulfur atoms for the biosynthesis of vitamins or other cofactors, or having protein-stabilizing effects. Hence, metalloproteins containing these cofactors are essential for numerous and diverse metabolic pathways and cellular processes occurring in the cytoplasm. Mitochondria are organelles where the Fe–S cluster demand is high, notably because the activity of the respiratory chain complexes I, II, and III relies on the correct assembly and functioning of Fe–S proteins. Several other proteins or complexes present in the matrix require Fe–S clusters as well, or depend either on Fe–S proteins such as ferredoxins or on cofactors such as lipoic acid or biotin whose synthesis relies on Fe–S proteins. In this review, we have listed and discussed the Fe–S-dependent enzymes or pathways in plant mitochondria including some potentially novel Fe–S proteins identified based on in silico analysis or on recent evidence obtained in non-plant organisms. We also provide information about recent developments concerning the molecular mechanisms involved in Fe–S cluster synthesis and trafficking steps of these cofactors from maturation factors to client apoproteins.},
	language = {en},
	number = {6},
	urldate = {2021-06-07},
	journal = {Journal of Experimental Botany},
	author = {Przybyla-Toscano, Jonathan and Christ, Loïck and Keech, Olivier and Rouhier, Nicolas},
	editor = {Dietz, Karl-Josef},
	month = mar,
	year = {2021},
	pages = {2014--2044},
}

2020 (4)

Centralization Within Sub-Experiments Enhances the Biological Relevance of Gene Co-expression Networks: A Plant Mitochondrial Case Study. Law, S. R., Kellgren, T. G., Björk, R., Ryden, P., & Keech, O. Frontiers in Plant Science, 11: 524. June 2020.

Centralization Within Sub-Experiments Enhances the Biological Relevance of Gene Co-expression Networks: A Plant Mitochondrial Case Study [link]

Paper doi link bibtex

@article{law_centralization_2020,
	title = {Centralization {Within} {Sub}-{Experiments} {Enhances} the {Biological} {Relevance} of {Gene} {Co}-expression {Networks}: {A} {Plant} {Mitochondrial} {Case} {Study}},
	volume = {11},
	issn = {1664-462X},
	shorttitle = {Centralization {Within} {Sub}-{Experiments} {Enhances} the {Biological} {Relevance} of {Gene} {Co}-expression {Networks}},
	url = {https://www.frontiersin.org/article/10.3389/fpls.2020.00524/full},
	doi = {10.3389/fpls.2020.00524},
	urldate = {2021-06-07},
	journal = {Frontiers in Plant Science},
	author = {Law, Simon R. and Kellgren, Therese G. and Björk, Rafael and Ryden, Patrik and Keech, Olivier},
	month = jun,
	year = {2020},
	pages = {524},
}

Siberian larch (Larix sibirica Ledeb.) mitochondrial genome assembled using both short and long nucleotide sequence reads is currently the largest known mitogenome. Putintseva, Y. A., Bondar, E. I., Simonov, E. P., Sharov, V. V., Oreshkova, N. V., Kuzmin, D. A., Konstantinov, Y. M., Shmakov, V. N., Belkov, V. I., Sadovsky, M. G., Keech, O., & Krutovsky, K. V. BMC Genomics, 21(1): 654. December 2020.

Paper doi link bibtex abstract

@article{putintseva_siberian_2020,
	title = {Siberian larch ({Larix} sibirica {Ledeb}.) mitochondrial genome assembled using both short and long nucleotide sequence reads is currently the largest known mitogenome},
	volume = {21},
	issn = {1471-2164},
	url = {https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-020-07061-4},
	doi = {10.1186/s12864-020-07061-4},
	abstract = {Abstract
            
              Background
              
                Plant mitochondrial genomes (mitogenomes) can be structurally complex while their size can vary from {\textasciitilde} 222 Kbp in
                Brassica napus
                to 11.3 Mbp in
                Silene conica
                . To date, in comparison with the number of plant species, only a few plant mitogenomes have been sequenced and released, particularly for conifers (the Pinaceae family). Conifers cover an ancient group of land plants that includes about 600 species, and which are of great ecological and economical value. Among them, Siberian larch (
                Larix sibirica
                Ledeb.) represents one of the keystone species in Siberian boreal forests. Yet, despite its importance for evolutionary and population studies, the mitogenome of Siberian larch has not yet been assembled and studied.
              
            
            
              Results
              Two sources of DNA sequences were used to search for mitochondrial DNA (mtDNA) sequences: mtDNA enriched samples and nucleotide reads generated in the de novo whole genome sequencing project, respectively. The assembly of the Siberian larch mitogenome contained nine contigs, with the shortest and the largest contigs being 24,767 bp and 4,008,762 bp, respectively. The total size of the genome was estimated at 11.7 Mbp. In total, 40 protein-coding, 34 tRNA, and 3 rRNA genes and numerous repetitive elements (REs) were annotated in this mitogenome. In total, 864 C-to-U RNA editing sites were found for 38 out of 40 protein-coding genes. The immense size of this genome, currently the largest reported, can be partly explained by variable numbers of mobile genetic elements, and introns, but unlikely by plasmid-related sequences. We found few plasmid-like insertions representing only 0.11\% of the entire Siberian larch mitogenome.
            
            
              Conclusions
              
                Our study showed that the size of the Siberian larch mitogenome is much larger than in other so far studied Gymnosperms, and in the same range as for the annual flowering plant
                Silene conica
                (11.3 Mbp). Similar to other species, the Siberian larch mitogenome contains relatively few genes, and despite its huge size, the repeated and low complexity regions cover only 14.46\% of the mitogenome sequence.},
	language = {en},
	number = {1},
	urldate = {2021-06-07},
	journal = {BMC Genomics},
	author = {Putintseva, Yuliya A. and Bondar, Eugeniya I. and Simonov, Evgeniy P. and Sharov, Vadim V. and Oreshkova, Natalya V. and Kuzmin, Dmitry A. and Konstantinov, Yuri M. and Shmakov, Vladimir N. and Belkov, Vadim I. and Sadovsky, Michael G. and Keech, Olivier and Krutovsky, Konstantin V.},
	month = dec,
	year = {2020},
	pages = {654},
}

Abstract Background Plant mitochondrial genomes (mitogenomes) can be structurally complex while their size can vary from ~ 222 Kbp in Brassica napus to 11.3 Mbp in Silene conica . To date, in comparison with the number of plant species, only a few plant mitogenomes have been sequenced and released, particularly for conifers (the Pinaceae family). Conifers cover an ancient group of land plants that includes about 600 species, and which are of great ecological and economical value. Among them, Siberian larch ( Larix sibirica Ledeb.) represents one of the keystone species in Siberian boreal forests. Yet, despite its importance for evolutionary and population studies, the mitogenome of Siberian larch has not yet been assembled and studied. Results Two sources of DNA sequences were used to search for mitochondrial DNA (mtDNA) sequences: mtDNA enriched samples and nucleotide reads generated in the de novo whole genome sequencing project, respectively. The assembly of the Siberian larch mitogenome contained nine contigs, with the shortest and the largest contigs being 24,767 bp and 4,008,762 bp, respectively. The total size of the genome was estimated at 11.7 Mbp. In total, 40 protein-coding, 34 tRNA, and 3 rRNA genes and numerous repetitive elements (REs) were annotated in this mitogenome. In total, 864 C-to-U RNA editing sites were found for 38 out of 40 protein-coding genes. The immense size of this genome, currently the largest reported, can be partly explained by variable numbers of mobile genetic elements, and introns, but unlikely by plasmid-related sequences. We found few plasmid-like insertions representing only 0.11% of the entire Siberian larch mitogenome. Conclusions Our study showed that the size of the Siberian larch mitogenome is much larger than in other so far studied Gymnosperms, and in the same range as for the annual flowering plant Silene conica (11.3 Mbp). Similar to other species, the Siberian larch mitogenome contains relatively few genes, and despite its huge size, the repeated and low complexity regions cover only 14.46% of the mitogenome sequence.

The Mitogenome of Norway Spruce and a Reappraisal of Mitochondrial Recombination in Plants. Sullivan, A. R, Eldfjell, Y., Schiffthaler, B., Delhomme, N., Asp, T., Hebelstrup, K. H, Keech, O., Öberg, L., Møller, I. M., Arvestad, L., Street, N. R, & Wang, X. Genome Biology and Evolution, 12(1): 3586–3598. January 2020.

The Mitogenome of Norway Spruce and a Reappraisal of Mitochondrial Recombination in Plants [link]

Paper doi link bibtex abstract

@article{sullivan_mitogenome_2020,
	title = {The {Mitogenome} of {Norway} {Spruce} and a {Reappraisal} of {Mitochondrial} {Recombination} in {Plants}},
	volume = {12},
	issn = {1759-6653},
	url = {https://academic.oup.com/gbe/article/12/1/3586/5644343},
	doi = {10.1093/gbe/evz263},
	abstract = {Abstract
            Plant mitogenomes can be difficult to assemble because they are structurally dynamic and prone to intergenomic DNA transfers, leading to the unusual situation where an organelle genome is far outnumbered by its nuclear counterparts. As a result, comparative mitogenome studies are in their infancy and some key aspects of genome evolution are still known mainly from pregenomic, qualitative methods. To help address these limitations, we combined machine learning and in silico enrichment of mitochondrial-like long reads to assemble the bacterial-sized mitogenome of Norway spruce (Pinaceae: Picea abies). We conducted comparative analyses of repeat abundance, intergenomic transfers, substitution and rearrangement rates, and estimated repeat-by-repeat homologous recombination rates. Prompted by our discovery of highly recombinogenic small repeats in P. abies, we assessed the genomic support for the prevailing hypothesis that intramolecular recombination is predominantly driven by repeat length, with larger repeats facilitating DNA exchange more readily. Overall, we found mixed support for this view: Recombination dynamics were heterogeneous across vascular plants and highly active small repeats (ca. 200 bp) were present in about one-third of studied mitogenomes. As in previous studies, we did not observe any robust relationships among commonly studied genome attributes, but we identify variation in recombination rates as a underinvestigated source of plant mitogenome diversity.},
	language = {en},
	number = {1},
	urldate = {2021-06-07},
	journal = {Genome Biology and Evolution},
	author = {Sullivan, Alexis R and Eldfjell, Yrin and Schiffthaler, Bastian and Delhomme, Nicolas and Asp, Torben and Hebelstrup, Kim H and Keech, Olivier and Öberg, Lisa and Møller, Ian Max and Arvestad, Lars and Street, Nathaniel R and Wang, Xiao-Ru},
	editor = {Vision, Todd},
	month = jan,
	year = {2020},
	pages = {3586--3598},
}

Tissue‐specific isolation of Arabidopsis/plant mitochondria – IMTACT (isolation of mitochondria tagged in specific cell types). Boussardon, C., Przybyla‐Toscano, J., Carrie, C., & Keech, O. The Plant Journal, 103(1): 459–473. July 2020.

Tissue‐specific isolation of Arabidopsis/plant mitochondria – IMTACT (isolation of mitochondria tagged in specific cell types) [link]

Paper doi link bibtex

@article{boussardon_tissuespecific_2020,
	title = {Tissue‐specific isolation of {Arabidopsis}/plant mitochondria – {IMTACT} (isolation of mitochondria tagged in specific cell types)},
	volume = {103},
	issn = {0960-7412, 1365-313X},
	url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/tpj.14723},
	doi = {10.1111/tpj.14723},
	language = {en},
	number = {1},
	urldate = {2021-06-07},
	journal = {The Plant Journal},
	author = {Boussardon, Clément and Przybyla‐Toscano, Jonathan and Carrie, Chris and Keech, Olivier},
	month = jul,
	year = {2020},
	pages = {459--473},
}

2019 (1)

Functional, Structural and Biochemical Features of Plant Serinyl-Glutathione Transferases. Sylvestre-Gonon, E., Law, S. R., Schwartz, M., Robe, K., Keech, O., Didierjean, C., Dubos, C., Rouhier, N., & Hecker, A. Frontiers in Plant Science, 10: 608. May 2019.

Functional, Structural and Biochemical Features of Plant Serinyl-Glutathione Transferases [link]

Paper doi link bibtex

@article{sylvestre-gonon_functional_2019,
	title = {Functional, {Structural} and {Biochemical} {Features} of {Plant} {Serinyl}-{Glutathione} {Transferases}},
	volume = {10},
	issn = {1664-462X},
	url = {https://www.frontiersin.org/article/10.3389/fpls.2019.00608/full},
	doi = {10/gjdxch},
	urldate = {2021-06-07},
	journal = {Frontiers in Plant Science},
	author = {Sylvestre-Gonon, Elodie and Law, Simon R. and Schwartz, Mathieu and Robe, Kevin and Keech, Olivier and Didierjean, Claude and Dubos, Christian and Rouhier, Nicolas and Hecker, Arnaud},
	month = may,
	year = {2019},
	pages = {608},
}

2018 (1)

Darkened Leaves Use Different Metabolic Strategies for Senescence and Survival. Law, S. R., Chrobok, D., Juvany, M., Delhomme, N., Lindén, P., Brouwer, B., Ahad, A., Moritz, T., Jansson, S., Gardeström, P., & Keech, O. Plant Physiology, 177(1): 132–150. May 2018.

Darkened Leaves Use Different Metabolic Strategies for Senescence and Survival [link]

Paper doi link bibtex

@article{law_darkened_2018,
	title = {Darkened {Leaves} {Use} {Different} {Metabolic} {Strategies} for {Senescence} and {Survival}},
	volume = {177},
	issn = {0032-0889, 1532-2548},
	url = {https://academic.oup.com/plphys/article/177/1/132-150/6116945},
	doi = {10.1104/pp.18.00062},
	language = {en},
	number = {1},
	urldate = {2021-06-07},
	journal = {Plant Physiology},
	author = {Law, Simon R. and Chrobok, Daria and Juvany, Marta and Delhomme, Nicolas and Lindén, Pernilla and Brouwer, Bastiaan and Ahad, Abdul and Moritz, Thomas and Jansson, Stefan and Gardeström, Per and Keech, Olivier},
	month = may,
	year = {2018},
	pages = {132--150},
}

2017 (2)

In Vitro Alkylation Methods for Assessing the Protein Redox State. Zannini, F., Couturier, J., Keech, O., & Rouhier, N. In Fernie, A. R., Bauwe, H., & Weber, A. P., editor(s), Photorespiration, volume 1653, pages 51–64. Springer New York, New York, NY, 2017. Series Title: Methods in Molecular Biology

In Vitro Alkylation Methods for Assessing the Protein Redox State [link]

Paper doi link bibtex

@incollection{fernie_vitro_2017,
	address = {New York, NY},
	title = {In {Vitro} {Alkylation} {Methods} for {Assessing} the {Protein} {Redox} {State}},
	volume = {1653},
	isbn = {978-1-4939-7224-1 978-1-4939-7225-8},
	url = {http://link.springer.com/10.1007/978-1-4939-7225-8_4},
	urldate = {2021-06-07},
	booktitle = {Photorespiration},
	publisher = {Springer New York},
	author = {Zannini, Flavien and Couturier, Jérémy and Keech, Olivier and Rouhier, Nicolas},
	editor = {Fernie, Alisdair R. and Bauwe, Hermann and Weber, Andreas P.M.},
	year = {2017},
	doi = {10.1007/978-1-4939-7225-8_4},
	note = {Series Title: Methods in Molecular Biology},
	pages = {51--64},
}

The redox control of photorespiration: from biochemical and physiological aspects to biotechnological considerations. Keech, O., Gardeström, P., Kleczkowski, L. A., & Rouhier, N. Plant, Cell & Environment, 40(4): 553–569. April 2017.

The redox control of photorespiration: from biochemical and physiological aspects to biotechnological considerations [link]

Paper doi link bibtex

@article{keech_redox_2017,
	title = {The redox control of photorespiration: from biochemical and physiological aspects to biotechnological considerations},
	volume = {40},
	issn = {0140-7791, 1365-3040},
	shorttitle = {The redox control of photorespiration},
	url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/pce.12713},
	doi = {10.1111/pce.12713},
	language = {en},
	number = {4},
	urldate = {2021-06-07},
	journal = {Plant, Cell \& Environment},
	author = {Keech, Olivier and Gardeström, Per and Kleczkowski, Leszek A. and Rouhier, Nicolas},
	month = apr,
	year = {2017},
	pages = {553--569},
}

2016 (7)

Characterization of a novel β-barrel protein (AtOM47) from the mitochondrial outer membrane of Arabidopsis thaliana. Li, L., Kubiszewski-Jakubiak, S., Radomiljac, J., Wang, Y., Law, S. R., Keech, O., Narsai, R., Berkowitz, O., Duncan, O., Murcha, M. W., & Whelan, J. Journal of Experimental Botany, 67(21): 6061–6075. November 2016.

Characterization of a novel β-barrel protein (AtOM47) from the mitochondrial outer membrane of <i>Arabidopsis thaliana</i> [link]

Paper doi link bibtex

@article{li_characterization_2016,
	title = {Characterization of a novel β-barrel protein ({AtOM47}) from the mitochondrial outer membrane of \textit{{Arabidopsis} thaliana}},
	volume = {67},
	issn = {0022-0957, 1460-2431},
	url = {https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/erw366},
	doi = {10/f9c9wf},
	language = {en},
	number = {21},
	urldate = {2021-06-07},
	journal = {Journal of Experimental Botany},
	author = {Li, Lu and Kubiszewski-Jakubiak, Szymon and Radomiljac, Jordan and Wang, Yan and Law, Simon R. and Keech, Olivier and Narsai, Reena and Berkowitz, Oliver and Duncan, Owen and Murcha, Monika W. and Whelan, James},
	month = nov,
	year = {2016},
	pages = {6061--6075},
}

Dark‐induced leaf senescence: new insights into a complex light‐dependent regulatory pathway. Liebsch, D., & Keech, O. New Phytologist, 212(3): 563–570. November 2016.

Dark‐induced leaf senescence: new insights into a complex light‐dependent regulatory pathway [link]

Paper doi link bibtex

@article{liebsch_darkinduced_2016,
	title = {Dark‐induced leaf senescence: new insights into a complex light‐dependent regulatory pathway},
	volume = {212},
	issn = {0028-646X, 1469-8137},
	shorttitle = {Dark‐induced leaf senescence},
	url = {https://onlinelibrary.wiley.com/doi/10.1111/nph.14217},
	doi = {10/f3trh7},
	language = {en},
	number = {3},
	urldate = {2021-06-07},
	journal = {New Phytologist},
	author = {Liebsch, Daniela and Keech, Olivier},
	month = nov,
	year = {2016},
	pages = {563--570},
}

Dissecting the Metabolic Role of Mitochondria during Developmental Leaf Senescence. Chrobok, D., Law, S. R., Brouwer, B., Lindén, P., Ziolkowska, A., Liebsch, D., Narsai, R., Szal, B., Moritz, T., Rouhier, N., Whelan, J., Gardeström, P., & Keech, O. Plant Physiology, 172(4): 2132–2153. December 2016.

Dissecting the Metabolic Role of Mitochondria during Developmental Leaf Senescence [link]

Paper doi link bibtex

@article{chrobok_dissecting_2016,
	title = {Dissecting the {Metabolic} {Role} of {Mitochondria} during {Developmental} {Leaf} {Senescence}},
	volume = {172},
	issn = {0032-0889, 1532-2548},
	url = {https://academic.oup.com/plphys/article/172/4/2132-2153/6115841},
	doi = {10/f3vc6g},
	language = {en},
	number = {4},
	urldate = {2021-06-07},
	journal = {Plant Physiology},
	author = {Chrobok, Daria and Law, Simon R. and Brouwer, Bastiaan and Lindén, Pernilla and Ziolkowska, Agnieszka and Liebsch, Daniela and Narsai, Reena and Szal, Bozena and Moritz, Thomas and Rouhier, Nicolas and Whelan, James and Gardeström, Per and Keech, Olivier},
	month = dec,
	year = {2016},
	pages = {2132--2153},
}

Manipulating photorespiration to increase plant productivity: recent advances and perspectives for crop improvement. Betti, M., Bauwe, H., Busch, F. A., Fernie, A. R., Keech, O., Levey, M., Ort, D. R., Parry, M. A. J., Sage, R., Timm, S., Walker, B., & Weber, A. P. M. Journal of Experimental Botany, 67(10): 2977–2988. May 2016.

Manipulating photorespiration to increase plant productivity: recent advances and perspectives for crop improvement [link]

Paper doi link bibtex

@article{betti_manipulating_2016,
	title = {Manipulating photorespiration to increase plant productivity: recent advances and perspectives for crop improvement},
	volume = {67},
	issn = {0022-0957, 1460-2431},
	shorttitle = {Manipulating photorespiration to increase plant productivity},
	url = {https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/erw076},
	doi = {10.1093/jxb/erw076},
	language = {en},
	number = {10},
	urldate = {2021-06-07},
	journal = {Journal of Experimental Botany},
	author = {Betti, Marco and Bauwe, Hermann and Busch, Florian A. and Fernie, Alisdair R. and Keech, Olivier and Levey, Myles and Ort, Donald R. and Parry, Martin A. J. and Sage, Rowan and Timm, Stefan and Walker, Berkley and Weber, Andreas P. M.},
	month = may,
	year = {2016},
	pages = {2977--2988},
}

Mitochondrial uncouplers inhibit clathrin-mediated endocytosis largely through cytoplasmic acidification. Dejonghe, W., Kuenen, S., Mylle, E., Vasileva, M., Keech, O., Viotti, C., Swerts, J., Fendrych, M., Ortiz-Morea, F. A., Mishev, K., Delang, S., Scholl, S., Zarza, X., Heilmann, M., Kourelis, J., Kasprowicz, J., Nguyen, L. S. L., Drozdzecki, A., Van Houtte, I., Szatmári, A., Majda, M., Baisa, G., Bednarek, S. Y., Robert, S., Audenaert, D., Testerink, C., Munnik, T., Van Damme, D., Heilmann, I., Schumacher, K., Winne, J., Friml, J., Verstreken, P., & Russinova, E. Nature Communications, 7(1): 11710. September 2016.

Mitochondrial uncouplers inhibit clathrin-mediated endocytosis largely through cytoplasmic acidification [link]

Paper doi link bibtex

@article{dejonghe_mitochondrial_2016,
	title = {Mitochondrial uncouplers inhibit clathrin-mediated endocytosis largely through cytoplasmic acidification},
	volume = {7},
	issn = {2041-1723},
	url = {http://www.nature.com/articles/ncomms11710},
	doi = {10/f3r3j2},
	language = {en},
	number = {1},
	urldate = {2021-06-07},
	journal = {Nature Communications},
	author = {Dejonghe, Wim and Kuenen, Sabine and Mylle, Evelien and Vasileva, Mina and Keech, Olivier and Viotti, Corrado and Swerts, Jef and Fendrych, Matyáš and Ortiz-Morea, Fausto Andres and Mishev, Kiril and Delang, Simon and Scholl, Stefan and Zarza, Xavier and Heilmann, Mareike and Kourelis, Jiorgos and Kasprowicz, Jaroslaw and Nguyen, Le Son Long and Drozdzecki, Andrzej and Van Houtte, Isabelle and Szatmári, Anna-Mária and Majda, Mateusz and Baisa, Gary and Bednarek, Sebastian York and Robert, Stéphanie and Audenaert, Dominique and Testerink, Christa and Munnik, Teun and Van Damme, Daniël and Heilmann, Ingo and Schumacher, Karin and Winne, Johan and Friml, Jiří and Verstreken, Patrik and Russinova, Eugenia},
	month = sep,
	year = {2016},
	pages = {11710},
}

Perspectives for a better understanding of the metabolic integration of photorespiration within a complex plant primary metabolism network. Hodges, M., Dellero, Y., Keech, O., Betti, M., Raghavendra, A. S., Sage, R., Zhu, X., Allen, D. K., & Weber, A. P. Journal of Experimental Botany, 67(10): 3015–3026. May 2016.

Paper doi link bibtex

@article{hodges_perspectives_2016,
	title = {Perspectives for a better understanding of the metabolic integration of photorespiration within a complex plant primary metabolism network},
	volume = {67},
	issn = {0022-0957, 1460-2431},
	url = {https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/erw145},
	doi = {10.1093/jxb/erw145},
	language = {en},
	number = {10},
	urldate = {2021-06-07},
	journal = {Journal of Experimental Botany},
	author = {Hodges, Michael and Dellero, Younès and Keech, Olivier and Betti, Marco and Raghavendra, Agepati S. and Sage, Rowan and Zhu, Xin-Guang and Allen, Doug K. and Weber, Andreas P.M.},
	month = may,
	year = {2016},
	pages = {3015--3026},
}

Reduced mitochondrial malate dehydrogenase activity has a strong effect on photorespiratory metabolism as revealed by $^{\textrm{13}}$ C labelling. Lindén, P., Keech, O., Stenlund, H., Gardeström, P., & Moritz, T. Journal of Experimental Botany, 67(10): 3123–3135. May 2016.
$Reduced mitochondrial malate dehydrogenase activity has a strong effect on photorespiratory metabolism as revealed by $^{\textrm{13}}$ C labelling [link]$ Paper doi link bibtex

@article{linden_reduced_2016,
	title = {Reduced mitochondrial malate dehydrogenase activity has a strong effect on photorespiratory metabolism as revealed by $^{\textrm{13}}$ {C} labelling},
	volume = {67},
	issn = {0022-0957, 1460-2431},
	url = {https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/erw030},
	doi = {10.1093/jxb/erw030},
	language = {en},
	number = {10},
	urldate = {2021-06-07},
	journal = {Journal of Experimental Botany},
	author = {Lindén, Pernilla and Keech, Olivier and Stenlund, Hans and Gardeström, Per and Moritz, Thomas},
	month = may,
	year = {2016},
	pages = {3123--3135},
}

2014 (2)

In response to partial plant shading, the lack of phytochrome A does not directly induce leaf senescence but alters the fine-tuning of chlorophyll biosynthesis. Brouwer, B., Gardeström, P., & Keech, O. Journal of Experimental Botany, 65(14): 4037–4049. July 2014.

Paper doi link bibtex

@article{brouwer_response_2014,
	title = {In response to partial plant shading, the lack of phytochrome {A} does not directly induce leaf senescence but alters the fine-tuning of chlorophyll biosynthesis},
	volume = {65},
	issn = {1460-2431, 0022-0957},
	url = {https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/eru060},
	doi = {10/f22wjn},
	language = {en},
	number = {14},
	urldate = {2021-06-08},
	journal = {Journal of Experimental Botany},
	author = {Brouwer, Bastiaan and Gardeström, Per and Keech, Olivier},
	month = jul,
	year = {2014},
	pages = {4037--4049},
}

The still mysterious roles of cysteine-containing glutathione transferases in plants. Lallement, P., Brouwer, B., Keech, O., Hecker, A., & Rouhier, N. Frontiers in Pharmacology, 5. August 2014.

The still mysterious roles of cysteine-containing glutathione transferases in plants [link]

Paper doi link bibtex

@article{lallement_still_2014,
	title = {The still mysterious roles of cysteine-containing glutathione transferases in plants},
	volume = {5},
	issn = {1663-9812},
	url = {http://journal.frontiersin.org/article/10.3389/fphar.2014.00192/abstract},
	doi = {10/f3n4qc},
	urldate = {2021-06-08},
	journal = {Frontiers in Pharmacology},
	author = {Lallement, Pierre-Alexandre and Brouwer, Bastiaan and Keech, Olivier and Hecker, Arnaud and Rouhier, Nicolas},
	month = aug,
	year = {2014},
}

2013 (4)

Engineering photorespiration: current state and future possibilities. Peterhansel, C., Krause, K., Braun, H., Espie, G. S., Fernie, A. R., Hanson, D. T., Keech, O., Maurino, V. G., Mielewczik, M., & Sage, R. F. Plant Biology, 15(4): 754–758. July 2013.

Engineering photorespiration: current state and future possibilities [link]

Paper doi link bibtex

@article{peterhansel_engineering_2013,
	title = {Engineering photorespiration: current state and future possibilities},
	volume = {15},
	issn = {14358603},
	shorttitle = {Engineering photorespiration},
	url = {http://doi.wiley.com/10.1111/j.1438-8677.2012.00681.x},
	doi = {10/f22k83},
	language = {en},
	number = {4},
	urldate = {2021-06-08},
	journal = {Plant Biology},
	author = {Peterhansel, C. and Krause, K. and Braun, H.-P. and Espie, G. S. and Fernie, A. R. and Hanson, D. T. and Keech, O. and Maurino, V. G. and Mielewczik, M. and Sage, R. F.},
	month = jul,
	year = {2013},
	pages = {754--758},
}

Perspectives on plant photorespiratory metabolism. Fernie, A. R., Bauwe, H., Eisenhut, M., Florian, A., Hanson, D. T., Hagemann, M., Keech, O., Mielewczik, M., Nikoloski, Z., Peterhänsel, C., Roje, S., Sage, R., Timm, S., von Cammerer, S., Weber, A. P. M., & Westhoff, P. Plant Biology, 15(4): 748–753. July 2013.

Perspectives on plant photorespiratory metabolism [link]

Paper doi link bibtex

@article{fernie_perspectives_2013,
	title = {Perspectives on plant photorespiratory metabolism},
	volume = {15},
	issn = {14358603},
	url = {http://doi.wiley.com/10.1111/j.1438-8677.2012.00693.x},
	doi = {10/f2364c},
	language = {en},
	number = {4},
	urldate = {2021-06-08},
	journal = {Plant Biology},
	author = {Fernie, A. R. and Bauwe, H. and Eisenhut, M. and Florian, A. and Hanson, D. T. and Hagemann, M. and Keech, O. and Mielewczik, M. and Nikoloski, Z. and Peterhänsel, C. and Roje, S. and Sage, R. and Timm, S. and von Cammerer, S. and Weber, A. P. M. and Westhoff, P.},
	editor = {Rennenberg, H.},
	month = jul,
	year = {2013},
	pages = {748--753},
}

Requirement for the plastidial oxidative pentose phosphate pathway for nitrate assimilation in Arabidopsis. Bussell, J. D., Keech, O., Fenske, R., & Smith, S. M. The Plant Journal, 75(4): 578–591. 2013.

Requirement for the plastidial oxidative pentose phosphate pathway for nitrate assimilation in Arabidopsis [link]

Paper doi link bibtex abstract

@article{bussell_requirement_2013,
	title = {Requirement for the plastidial oxidative pentose phosphate pathway for nitrate assimilation in {Arabidopsis}},
	volume = {75},
	copyright = {© 2013 The Authors The Plant Journal © 2013 John Wiley \& Sons Ltd},
	issn = {1365-313X},
	url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/tpj.12222},
	doi = {10/f46xkb},
	abstract = {Sugar metabolism and the oxidative pentose phosphate pathway (OPPP) are strongly implicated in N assimilation, although the relationship between them and the roles of the plastidial and cytosolic OPPP have not been established genetically. We studied a knock-down mutant of the plastid-localized OPPP enzyme 6-phosphogluconolactonase 3 (PGL3). pgl3-1 plants exhibited relatively greater resource allocation to roots but were smaller than the wild type. They had a lower content of amino acids and free in leaves than the wild type, despite exhibiting comparable photosynthetic rates and efficiency, and normal levels of many other primary metabolites. When N-deprived plants were fed via the roots with , pgl3-1 exhibited normal induction of OPPP and nitrate assimilation genes in roots, and amino acids in roots and shoots were labeled with 15N at least as rapidly as in the wild type. However, when N-replete plants were fed via the roots with sucrose, expression of specific OPPP and N assimilation genes in roots increased in the wild type but not in pgl3-1. Thus, sugar-dependent expression of N assimilation genes requires OPPP activity and the specificity of the effect of the pgl3-1 mutation on N assimilation genes establishes that it is not the result of general energy deficiency or accumulation of toxic intermediates. We conclude that expression of specific nitrate assimilation genes in the nucleus of root cells is positively regulated by a signal emanating from OPPP activity in the plastid.},
	language = {en},
	number = {4},
	urldate = {2021-06-10},
	journal = {The Plant Journal},
	author = {Bussell, John D. and Keech, Olivier and Fenske, Ricarda and Smith, Steven M.},
	year = {2013},
	keywords = {6-phosphogluconolactonase, Arabidopsis thaliana, nitrate, nitrogen assimilation, oxidative pentose phosphate pathway, plastid},
	pages = {578--591},
}

The Norway spruce genome sequence and conifer genome evolution. Nystedt, B., Street, N. R., Wetterbom, A., Zuccolo, A., Lin, Y., Scofield, D. G., Vezzi, F., Delhomme, N., Giacomello, S., Alexeyenko, A., Vicedomini, R., Sahlin, K., Sherwood, E., Elfstrand, M., Gramzow, L., Holmberg, K., Hällman, J., Keech, O., Klasson, L., Koriabine, M., Kucukoglu, M., Käller, M., Luthman, J., Lysholm, F., Niittylä, T., Olson, Å., Rilakovic, N., Ritland, C., Rosselló, J. A., Sena, J., Svensson, T., Talavera-López, C., Theißen, G., Tuominen, H., Vanneste, K., Wu, Z., Zhang, B., Zerbe, P., Arvestad, L., Bhalerao, R. P., Bohlmann, J., Bousquet, J., Garcia Gil, R., Hvidsten, T. R., de Jong, P., MacKay, J., Morgante, M., Ritland, K., Sundberg, B., Lee Thompson, S., Van de Peer, Y., Andersson, B., Nilsson, O., Ingvarsson, P. K., Lundeberg, J., & Jansson, S. Nature, 497(7451): 579–584. May 2013.

The Norway spruce genome sequence and conifer genome evolution [link]

Paper doi link bibtex

@article{nystedt_norway_2013,
	title = {The {Norway} spruce genome sequence and conifer genome evolution},
	volume = {497},
	issn = {0028-0836, 1476-4687},
	url = {http://www.nature.com/articles/nature12211},
	doi = {10/f2zsx6},
	language = {en},
	number = {7451},
	urldate = {2021-06-08},
	journal = {Nature},
	author = {Nystedt, Björn and Street, Nathaniel R. and Wetterbom, Anna and Zuccolo, Andrea and Lin, Yao-Cheng and Scofield, Douglas G. and Vezzi, Francesco and Delhomme, Nicolas and Giacomello, Stefania and Alexeyenko, Andrey and Vicedomini, Riccardo and Sahlin, Kristoffer and Sherwood, Ellen and Elfstrand, Malin and Gramzow, Lydia and Holmberg, Kristina and Hällman, Jimmie and Keech, Olivier and Klasson, Lisa and Koriabine, Maxim and Kucukoglu, Melis and Käller, Max and Luthman, Johannes and Lysholm, Fredrik and Niittylä, Totte and Olson, Åke and Rilakovic, Nemanja and Ritland, Carol and Rosselló, Josep A. and Sena, Juliana and Svensson, Thomas and Talavera-López, Carlos and Theißen, Günter and Tuominen, Hannele and Vanneste, Kevin and Wu, Zhi-Qiang and Zhang, Bo and Zerbe, Philipp and Arvestad, Lars and Bhalerao, Rishikesh P. and Bohlmann, Joerg and Bousquet, Jean and Garcia Gil, Rosario and Hvidsten, Torgeir R. and de Jong, Pieter and MacKay, John and Morgante, Michele and Ritland, Kermit and Sundberg, Björn and Lee Thompson, Stacey and Van de Peer, Yves and Andersson, Björn and Nilsson, Ove and Ingvarsson, Pär K. and Lundeberg, Joakim and Jansson, Stefan},
	month = may,
	year = {2013},
	pages = {579--584},
}

2012 (2)

The Genetic Dissection of a Short-Term Response to Low CO2 Supports the Possibility for Peroxide-Mediated Decarboxylation of Photorespiratory Intermediates in the Peroxisome. Keech, O., Zhou, W., Fenske, R., Colas-des-Francs-Small, C., Bussell, J. D., Badger, M. R., & Smith, S. M. Molecular Plant, 5(6): 1413–1416. November 2012.

Paper doi link bibtex

@article{keech_genetic_2012,
	title = {The {Genetic} {Dissection} of a {Short}-{Term} {Response} to {Low} {CO2} {Supports} the {Possibility} for {Peroxide}-{Mediated} {Decarboxylation} of {Photorespiratory} {Intermediates} in the {Peroxisome}},
	volume = {5},
	issn = {16742052},
	url = {https://linkinghub.elsevier.com/retrieve/pii/S1674205214601647},
	doi = {10/gkgdr5},
	language = {en},
	number = {6},
	urldate = {2021-06-08},
	journal = {Molecular Plant},
	author = {Keech, Olivier and Zhou, Wenxu and Fenske, Ricarda and Colas-des-Francs-Small, Catherine and Bussell, John D. and Badger, Murray R. and Smith, Steven M.},
	month = nov,
	year = {2012},
	pages = {1413--1416},
}

The impact of light intensity on shade-induced leaf senescence: Light-dependent induction of leaf senescence. Brouwer, B., Ziolkowska, A., Bagard, M., Keech, O., & Gardeström, P. Plant, Cell & Environment, 35(6): 1084–1098. June 2012.

The impact of light intensity on shade-induced leaf senescence: Light-dependent induction of leaf senescence [link]

Paper doi link bibtex

@article{brouwer_impact_2012,
	title = {The impact of light intensity on shade-induced leaf senescence: {Light}-dependent induction of leaf senescence},
	volume = {35},
	issn = {01407791},
	shorttitle = {The impact of light intensity on shade-induced leaf senescence},
	url = {http://doi.wiley.com/10.1111/j.1365-3040.2011.02474.x},
	doi = {10/dthghs},
	language = {en},
	number = {6},
	urldate = {2021-06-08},
	journal = {Plant, Cell \& Environment},
	author = {Brouwer, Bastiaan and Ziolkowska, Agnieszka and Bagard, Matthieu and Keech, Olivier and Gardeström, Per},
	month = jun,
	year = {2012},
	pages = {1084--1098},
}

2011 (1)

The conserved mobility of mitochondria during leaf senescence reflects differential regulation of the cytoskeletal components in Arabidopsis thaliana. Keech, O. Plant Signaling & Behavior, 6(1): 147–150. January 2011.

Paper doi link bibtex abstract

@article{keech_conserved_2011,
	title = {The conserved mobility of mitochondria during leaf senescence reflects differential regulation of the cytoskeletal components in {Arabidopsis} thaliana},
	volume = {6},
	issn = {null},
	url = {https://doi.org/10.4161/psb.6.1.14307},
	doi = {10/bzzb2q},
	abstract = {Leaf senescence is an organized process, which requires fine tuning between nuclear gene expression, activity of proteases and the maintenance of primary metabolism. Recently, we reported that leaf senescence was accompanied by an early degradation of the microtubule cytoskeleton in Arabidopsis thaliana. As the cytoskeleton is essential for cell stability, vesicle shuttling and organelle mobility, it might be asked how the regulation of these cell functions occurs with such drastic modifications of the cytoskeleton. Based on confocal laser microscopy observations and a micro-array analysis, the following addendum shows that mitochondrial mobility is conserved until the late stages of leaf senescence and provides evidences that the actin-cytoskeleton is maintained longer than the microtubule network. This conservation of actin-filaments is discussed with regards to energy metabolism as well as calcium signaling during programmed cell death.},
	number = {1},
	urldate = {2021-06-10},
	journal = {Plant Signaling \& Behavior},
	author = {Keech, Olivier},
	month = jan,
	year = {2011},
	pages = {147--150},
}

2010 (2)

Arabidopsis has a cytosolic fumarase required for the massive allocation of photosynthate into fumaric acid and for rapid plant growth on high nitrogen. Pracharoenwattana, I., Zhou, W., Keech, O., Francisco, P. B., Udomchalothorn, T., Tschoep, H., Stitt, M., Gibon, Y., & Smith, S. M. The Plant Journal, 62(5): 785–795. 2010.

Paper doi link bibtex abstract

@article{pracharoenwattana_arabidopsis_2010,
	title = {Arabidopsis has a cytosolic fumarase required for the massive allocation of photosynthate into fumaric acid and for rapid plant growth on high nitrogen},
	volume = {62},
	copyright = {© 2010 The Authors. Journal compilation © 2010 Blackwell Publishing Ltd},
	issn = {1365-313X},
	url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-313X.2010.04189.x},
	doi = {10/bpcb9c},
	abstract = {The Arabidopsis genome has two fumarase genes, one of which encodes a protein with mitochondrial targeting information (FUM1) while the other (FUM2) does not. We show that a FUM1–green fluorescent protein fusion is directed to mitochondria while FUM2–red fluorescent protein remains in the cytosol. While mitochondrial FUM1 is an essential gene, cytosolic FUM2 is not required for plant growth. However FUM2 is required for the massive accumulation of carbon into fumarate that occurs in Arabidopsis leaves during the day. In fum2 knock-out mutants, fumarate levels remain low while malate increases, and these changes can be reversed with a FUM2 transgene. The fum2 mutant has lower levels of many amino acids in leaves during the day compared with the wild type, but higher levels at night, consistent with a link between fumarate and amino acid metabolism. To further test this relationship we grew plants in the absence or presence of nitrogen fertilizer. The amount of fumarate in leaves increased several fold in response to nitrogen in wild-type plants, but not in fum2. Malate increased to a small extent in the wild type but to a greater extent in fum2. Growth of fum2 plants was similar to that of the wild type in low nitrogen but much slower in the presence of high nitrogen. Activities of key enzymes of nitrogen assimilation were similar in both genotypes. We conclude that FUM2 is required for the accumulation of fumarate in leaves, which is in turn required for rapid nitrogen assimilation and growth on high nitrogen.},
	language = {en},
	number = {5},
	urldate = {2021-06-10},
	journal = {The Plant Journal},
	author = {Pracharoenwattana, Itsara and Zhou, Wenxu and Keech, Olivier and Francisco, Perigio B. and Udomchalothorn, Thanikan and Tschoep, Hendrik and Stitt, Mark and Gibon, Yves and Smith, Steven M.},
	year = {2010},
	keywords = {Arabidopsis thaliana, fumarase, fumaric acid, nitrogen assimilation, photosynthate allocation, plant growth},
	pages = {785--795},
}

Leaf Senescence Is Accompanied by an Early Disruption of the Microtubule Network in Arabidopsis. Keech, O., Pesquet, E., Gutierrez, L., Ahad, A., Bellini, C., Smith, S. M., & Gardeström, P. Plant Physiology, 154(4): 1710–1720. December 2010.

Leaf Senescence Is Accompanied by an Early Disruption of the Microtubule Network in Arabidopsis [link]

Paper doi link bibtex abstract

@article{keech_leaf_2010,
	title = {Leaf {Senescence} {Is} {Accompanied} by an {Early} {Disruption} of the {Microtubule} {Network} in {Arabidopsis}},
	volume = {154},
	issn = {1532-2548},
	url = {https://academic.oup.com/plphys/article/154/4/1710/6108651},
	doi = {10/cp2qs5},
	abstract = {Abstract
            The dynamic assembly and disassembly of microtubules (MTs) is essential for cell function. Although leaf senescence is a well-documented process, the role of the MT cytoskeleton during senescence in plants remains unknown. Here, we show that both natural leaf senescence and senescence of individually darkened Arabidopsis (Arabidopsis thaliana) leaves are accompanied by early degradation of the MT network in epidermis and mesophyll cells, whereas guard cells, which do not senesce, retain their MT network. Similarly, entirely darkened plants, which do not senesce, retain their MT network. While genes encoding the tubulin subunits and the bundling/stabilizing MT-associated proteins (MAPs) MAP65 and MAP70-1 were repressed in both natural senescence and dark-induced senescence, we found strong induction of the gene encoding the MT-destabilizing protein MAP18. However, induction of MAP18 gene expression was also observed in leaves from entirely darkened plants, showing that its expression is not sufficient to induce MT disassembly and is more likely to be part of a Ca2+-dependent signaling mechanism. Similarly, genes encoding the MT-severing protein katanin p60 and two of the four putative regulatory katanin p80s were repressed in the dark, but their expression did not correlate with degradation of the MT network during leaf senescence. Taken together, these results highlight the earliness of the degradation of the cortical MT array during leaf senescence and lead us to propose a model in which suppression of tubulin and MAP genes together with induction of MAP18 play key roles in MT disassembly during senescence.},
	language = {en},
	number = {4},
	urldate = {2021-06-08},
	journal = {Plant Physiology},
	author = {Keech, Olivier and Pesquet, Edouard and Gutierrez, Laurent and Ahad, Abdul and Bellini, Catherine and Smith, Steven M. and Gardeström, Per},
	month = dec,
	year = {2010},
	pages = {1710--1720},
}

2009 (1)

Magic‐angle phosphorus NMR of functional mitochondria: in situ monitoring of lipid response under apoptotic‐like stress. Sani, M., Keech, O., Gardeström, P., Dufourc, E. J., & Gröbner, G. The FASEB Journal, 23(9): 2872–2878. September 2009.

Magic‐angle phosphorus NMR of functional mitochondria: <i>in situ</i> monitoring of lipid response under apoptotic‐like stress [link]

Paper doi link bibtex

@article{sani_magicangle_2009,
	title = {Magic‐angle phosphorus {NMR} of functional mitochondria: \textit{in situ} monitoring of lipid response under apoptotic‐like stress},
	volume = {23},
	issn = {0892-6638, 1530-6860},
	shorttitle = {Magic‐angle phosphorus {NMR} of functional mitochondria},
	url = {https://onlinelibrary.wiley.com/doi/abs/10.1096/fj.09-134114},
	doi = {10/b8cdxs},
	language = {en},
	number = {9},
	urldate = {2021-06-08},
	journal = {The FASEB Journal},
	author = {Sani, Marc‐Antoine and Keech, Olivier and Gardeström, Per and Dufourc, Erick J. and Gröbner, Gerhard},
	month = sep,
	year = {2009},
	pages = {2872--2878},
}

2007 (2)

The different fates of mitochondria and chloroplasts during dark-induced senescence in Arabidopsis leaves. Keech, O., Pesquet, E., Ahad, A., Askne, A., Nordvall, D., Vodnala, S. M., Tuominen, H., Hurry, V., Dizengremel, P., & Gardeström, P. Plant, Cell & Environment, 30(12): 1523–1534. December 2007.

The different fates of mitochondria and chloroplasts during dark-induced senescence in Arabidopsis leaves [link]

Paper doi link bibtex

@article{keech_different_2007,
	title = {The different fates of mitochondria and chloroplasts during dark-induced senescence in {Arabidopsis} leaves},
	volume = {30},
	issn = {0140-7791, 1365-3040},
	url = {http://doi.wiley.com/10.1111/j.1365-3040.2007.01724.x},
	doi = {10/bpfzq8},
	language = {en},
	number = {12},
	urldate = {2021-06-10},
	journal = {Plant, Cell \& Environment},
	author = {Keech, Olivier and Pesquet, Edouard and Ahad, Abdul and Askne, Anna and Nordvall, Dag and Vodnala, Sharvani Munender and Tuominen, Hannele and Hurry, Vaughan and Dizengremel, Pierre and Gardeström, Per},
	month = dec,
	year = {2007},
	pages = {1523--1534},
}

The mitochondrial type II peroxiredoxin from poplar. Gama, F., Keech, O., Eymery, F., Finkemeier, I., Gelhaye, E., Gardeström, P., Dietz, K. J., Rey, P., Jacquot, J., & Rouhier, N. Physiologia Plantarum, 129(1): 196–206. January 2007.

The mitochondrial type II peroxiredoxin from poplar [link]

Paper doi link bibtex

@article{gama_mitochondrial_2007,
	title = {The mitochondrial type {II} peroxiredoxin from poplar},
	volume = {129},
	issn = {0031-9317, 1399-3054},
	url = {http://doi.wiley.com/10.1111/j.1399-3054.2006.00785.x},
	doi = {10/d9wwkk},
	language = {en},
	number = {1},
	urldate = {2021-06-10},
	journal = {Physiologia Plantarum},
	author = {Gama, Filipe and Keech, Olivier and Eymery, Françoise and Finkemeier, Iris and Gelhaye, Eric and Gardeström, Per and Dietz, Karl Josef and Rey, Pascal and Jacquot, Jean-Pierre and Rouhier, Nicolas},
	month = jan,
	year = {2007},
	pages = {196--206},
}

2005 (3)

Adsorption of allelopathic compounds by wood-derived charcoal: the role of wood porosity. Keech, O., Carcaillet, C., & Nilsson, M. Plant and Soil, 272(1): 291–300. May 2005.

Adsorption of allelopathic compounds by wood-derived charcoal: the role of wood porosity [link]

Paper doi link bibtex abstract

@article{keech_adsorption_2005,
	title = {Adsorption of allelopathic compounds by wood-derived charcoal: the role of wood porosity},
	volume = {272},
	issn = {1573-5036},
	shorttitle = {Adsorption of allelopathic compounds by wood-derived charcoal},
	url = {https://doi.org/10.1007/s11104-004-5485-5},
	doi = {10.1007/s11104-004-5485-5},
	abstract = {In Swedish boreal forests, areas dominated by the dwarf shrub Empetrum hermaphroditum Hagerup are known for their poor regeneration of trees and one of the causes of this poor regeneration has been attributed to allelopathy (i.e. chemical interferences) by E. hermaphroditum. Fire-produced charcoal is suggested to play an important role in rejuvenating those ecosystems by adsorbing allelopathic compounds, such as phenols, released by E. hermaphroditum. In this study, we firstly investigated whether the adsorption capacity of charcoal of different plant species varies according to the wood anatomical structures of these, and secondly we tried to relate the adsorption capacity to wood anatomical structure. Charcoal was produced from eight boreal and one temperate woody plant species and the adsorption capacity of charcoal was tested by bioassays technique. Seed germination was used as a measurement of the ability of charcoal to adsorb allelochemicals. The charcoal porosity was estimated and the pore size distribution was then calculated in order to relate the wood anatomical features to the adsorption capacity. The results showed that the adsorption capacity of charcoal was significantly different between plant species and that deciduous trees had a significantly higher adsorption capacity than conifers and ericaceous species. The presence of macro-pores rather than a high porosity appears to be the most important for the adsorption capacity. These results suggest that fire-produced charcoal has different ability to adsorb phenols in boreal forest soil, and therefore may have differing effects on the germination of seeds of establishing tree seedlings.},
	language = {en},
	number = {1},
	urldate = {2021-06-11},
	journal = {Plant and Soil},
	author = {Keech, Olivier and Carcaillet, Christopher and Nilsson, Marie-Charlotte},
	month = may,
	year = {2005},
	pages = {291--300},
}

Identification of Plant Glutaredoxin Targets. Rouhier, N., Villarejo, A., Srivastava, M., Gelhaye, E., Keech, O., Droux, M., Finkemeier, I., Samuelsson, G., Dietz, K. J., Jacquot, J., & Wingsle, G. Antioxidants & Redox Signaling, 7(7-8): 919–929. July 2005. Publisher: Mary Ann Liebert, Inc., publishers

Identification of Plant Glutaredoxin Targets [link]

Paper doi link bibtex abstract

@article{rouhier_identification_2005,
	title = {Identification of {Plant} {Glutaredoxin} {Targets}},
	volume = {7},
	issn = {1523-0864},
	url = {https://www.liebertpub.com/doi/10.1089/ars.2005.7.919},
	doi = {10.1089/ars.2005.7.919},
	abstract = {Glutaredoxins (Grxs) are small ubiquitous proteins of the thioredoxin (Trx) family, which catalyze dithiol–disulfide exchange reactions or reduce protein-mixed glutathione disulfides. In plants, several Trx-interacting proteins have been isolated from different compartments, whereas very few Grx-interacting proteins are known. We describe here the determination of Grx target proteins using a mutated poplar Grx, various tissular and subcellular plant extracts, and liquid chromatography coupled to tandem mass spectrometry detection. We have identified 94 putative targets, involved in many processes, including oxidative stress response [peroxiredoxins (Prxs), ascorbate peroxidase, catalase], nitrogen, sulfur, and carbon metabolisms (methionine synthase, alanine aminotransferase, phosphoglycerate kinase), translation (elongation factors E and Tu), or protein folding (heat shock protein 70). Some of these proteins were previously found to interact with Trx or to be glutathiolated in other organisms, but others could be more specific partners of Grx. To substantiate further these data, Grx was shown to support catalysis of the stroma β-type carbonic anhydrase and Prx IIF of Arabidopsis thaliana, but not of poplar 2-Cys Prx. Overall, these data suggest that the interaction could occur randomly either with exposed cysteinyl disulfide bonds formed within or between target proteins or with mixed disulfides between a protein thiol and glutathione.Antioxid. Redox Signal. 7, 919–929.},
	number = {7-8},
	urldate = {2021-06-11},
	journal = {Antioxidants \& Redox Signaling},
	author = {Rouhier, Nicolas and Villarejo, Arsenio and Srivastava, Manoj and Gelhaye, Eric and Keech, Olivier and Droux, Michel and Finkemeier, Iris and Samuelsson, Göran and Dietz, Karl Josef and Jacquot, Jean-Pierre and Wingsle, Gunnar},
	month = jul,
	year = {2005},
	note = {Publisher: Mary Ann Liebert, Inc., publishers},
	pages = {919--929},
}

Preparation of leaf mitochondria from Arabidopsis thaliana. Keech, O., Dizengremel, P., & Gardestrom, P. Physiologia Plantarum, 124(4): 403–409. August 2005. Place: Hoboken Publisher: Wiley WOS:000230573300001
doi link bibtex abstract

@article{keech_preparation_2005,
	title = {Preparation of leaf mitochondria from {Arabidopsis} thaliana},
	volume = {124},
	issn = {0031-9317},
	doi = {10/d7jvrz},
	abstract = {Arabidopsis thaliana is, perhaps, the most important model species in modern plant biology. However, the isolation of organelles from leaves of this plant has been difficult. Here, we present two different protocols for the isolation of mitochondria, yielding either highly functional crude mitochondria or highly purified mitochondria. The crude mitochondria were well coupled with the substrates tested (malate + glutamate, glycine and NADH), exhibiting respiratory control ratios of 2.1-3.9. Purified mitochondria with very low levels of chlorophyll contamination were obtained by Percoll gradient centrifugation, yielding 1.2 mg of mitochondrial protein from 50 g of leaves.},
	language = {English},
	number = {4},
	journal = {Physiologia Plantarum},
	author = {Keech, O. and Dizengremel, P. and Gardestrom, P.},
	month = aug,
	year = {2005},
	note = {Place: Hoboken
Publisher: Wiley
WOS:000230573300001},
	keywords = {chloroplasts, criteria, dehydrogenase, expression, metabolism, oxidation, photosynthesis, respiration, spinach, tissue},
	pages = {403--409},
}