Request transformation or amplification

Use your normal UMU-ID or SLU-ID login to access the request form


Procedure for transformation request:

  • Fill in the request form and submit new request through the link requests.
  • Choose the construct name carefully, so that it is possible to identify the construct in the database in the future, both for yourself and for others. It is not possible to change the name later when we have started the transformation.
  • Your transformation will be assigned a database ID number by lab manager Veronica Bourquin.
  • The platform will notify you when your request is scheduled for transformation minimum 1 week in advance, so that you can prepare the bacteria culture.
  • One member of our team will be assigned to perform the transformation and the following cultivation of your construct.

Note! The requests will be handled in the order they are submitted to the platform.

Good to know prior to the day of transformation:

  • Bring your Agro pellet at 09:00-09:30 on the day of transformation (we do not accept arrivals later than 10:00!)
  • Prepare the pellet from a 50 ml growing culture with an OD600 of 0.5-0.8 (no lower than 0,3 and no higher than 1.5!)

After the transformation event, we will update you when it is time to sample and genotype the lines.Scheme illustrating the different steps of the poplar transformation procedure: first step is the infection of stem parts with Agrobacterium and selection of transgenes on plates; after two to six months, the transgenic calli will be then transferred to bigger jars for shoot elongation which takes another 1.5-2 months; the shoots will then be transferred to higher jars for root formation which lasts 2-3 months; finally, the sticklings will be transferred to a new jar - the "1st Mother jar" and propagated for 1-1.5monthsThe poplar transformation procedure: 2-6 months - Infection with Agrobacterium and selection of transgenes on plates; 1.5-2 months - Induction of shoot elongation in transgenic calli; 2-3 months - Transfer of shoots to new medium for root formation; 1-1.5 months: Transformation completed - the "1st Mother jar" is ready

Procedure for requesting poplar transformation:

Access the request form through the link above Request Amplification - New Request, Good to know prior to fill in the amplification request.

  • Use the construct ID number generated before the transformation and state which lines you want (one line per row)
    If you do not have the construct ID number, use the construct name originally submitted for the transformation and write that in the comments and we can help you find out the correct ID number!
  • Amount of plants
    We always propagate extra (approx. 10%) to increase the chance to meet the amount requested. (Write in the comments if you do not want extra plants).
  • Plants/jar: there will be three plants in each jar by default.
  • Wallenberg Greenhouse experiment: Announce whether the amplified lines are planned for potting in the Wallenberg Greenhouse (YES/NO). Note that this is not a booking! Please contact This email address is being protected from spambots. You need JavaScript enabled to view it. for booking space at Wallenberg, in climate chambers at UPSC floor 5 or at Skogis.
  • Homogenous (Yes/No), is homogeneity important for your experiment?

Note! Amplifications take time. For example, an amplification of 15 replicates generally needs 2-3 propagation rounds. First propagation takes at least 5 weeks to grow, the following takes about 4 weeks each. If you want them more homogeneous, 1 or 2 extra propagations may be needed. Also, if you want less than 3 plants in each jar by delivery it will need an extra propagation round.

Amplifieringsschedule, overview of poplar amplification procedureFirst cut is a kick start and takes about 4-6 weeks, then depending of how many replicates it need 1-3 more amplifications rounds. When we have the final number of plants we can make the fianl cut and then the amplification is ready usually between 8-27 weeks