Search Calendar
PhD half-time seminar: Dmity Kremnev
23-May-2012 16:00 - 17:00
Half-Time PhD seminar
Speaker
Dmitry Kremnev
Title:
PLASTID REDOX INSENSITIVE 2, is a putative regulatory component that controls PEP transcription
Dissertation - Bastiaan Brouwer
25-May-2012 13:00 - 16:00
Title: Shedding Light on Shade- and Dark-Induced Leaf Senescence
Place: KB3A9
Opponent:
Karin Krupinska, Professor
Department of Plant Cell Biology,
Christian-Albrechts-Universität Kiel/Kiel University
Supervisor: Per Gardeström
Cancelled!!!! Seminar with David Nelson
28-May-2012 15:00 - 16:00
David Nelson
From Department of genetics, University of Georgia, Athens, USA
Place: Lilla horsalen, 15.00
Title:
Smoke and mirrors: The curious connection between post-fire germination and shoot branching control
Abstract:
Karrikins are a class of butenolide compounds identified in smoke that have prominent roles in activating post-fire seed germination. Genetic studies performed in Arabidopsis thaliana demonstrated that the F-box protein MAX2 is required for karrikin responses. MAX2 is also necessary for responses to strigolactones, a class of plant hormones originally identified in host root exudates as highly potent germination stimulants of parasitic weeds. Strigolactones are also known to have roles in the control of shoot branching and recruitment of arbuscular mycorrhizal fungi - neither of which processes are influenced by karrikins. Two homologous proteins, KAI2 and D14, have now been shown to mediate strigolactone and karrikin-specific responses in Arabidopsis. Through genetic suppressor screens we are identifying additional components of the MAX2-dependent karrikin and strigolactone signaling pathways.
UPSC Seminar - Sergei Miroshnichenko:MAP20: study of a microtubule-associated protein in xylem
29-May-2012 15:00 - 16:00
Speaker
Sergeiy Miroshnichenko
Title:
MAP20: study of a microtubule-associated protein in xylem
Place: KB3B3
Seminar -Sacha Baginsky: Functional characterization of plant proteome dynamics
31-May-2012 14:00 - 15:00
Department of Chemistry
Seminar
Sacha Baginsky
Martin-Luther-Universität Halle-Wittenberg, Institut für Biochemie und Biotechnologie, Abteilung Pflanzenbiochemie
Title:
Functional characterization of plant proteome dynamics
Place : Lilla hörsalen, KB3A9, KBC
Host Christiane Funk
Abstract:
Different functional proteomics tools are now available that enable the quantitative characterization of proteome dynamics and the mapping of posttranslational modifications. We report here examples how we used these tools to characterize the functional proteome of Arabidopsis and rice cell organelles, with a focus on the plant-specific plastids. We analyzed the Arabidopsis proteome at genome-scale and provide quantitative information about organellar proteomes in different plant organs by "normalized spectral counting" (Science, 320: 938-41). For a functional characterization of plastid protein import, we analyzed the proteomes of plastid protein import mutants and searched for N-terminal acetylated peptides in genome-scale WT, ppi1 and ppi2 proteomics data. These analyses revealed the accumulation of precursor proteins in the cytosol of the import mutants. In order to assess the short-term regulation of the chloroplast proteome in response to environmental signals, we analyzed the chloroplast phosphoproteome and characterized its dynamics during a circadian cycle (Plant Physiol, 150: 889-903). Differential protein phosphorylation was assessed by relative protein quantification with "extracted ion chromatograms". We present here our data, comment on reliability and reproducibility and propose strategies to increase both at a reasonable cost.
Sacha Baginsky
Martin-Luther-Universität Halle-Wittenberg, Institut für Biochemie und Biotechnologie, Abteilung Pflanzenbiochemie, Weinbergweg 22 (Biozentrum),
06120 Halle (Saale)
Email:
Seminar - David Hodge: Understanding and Enhancing Alkaline and Oxidative Chemical Pretreatments for the Production of Cellulosic Biofuels through Improved Characterization
31-May-2012 15:00 - 16:00
Speaker:
David Hodge
Assistant Professor, Chemical Engineering and Materials Science, Biosystems and Agricultural Engineering
Michigan State University
Title
Understanding and Enhancing Alkaline and Oxidative Chemical Pretreatments for the Production of Cellulosic Biofuels through Improved Characterization
Host: Hannele Tuominen
Place: Seminar room KB3B3 at KBC
Abstract:
This seminar will present recent research on improving technologies for oxidative chemical pretreatments and alkaline fractionation of plant biomass. One theme underlying this research is how improved characterization of the chemical, structural, and physical changes to the plant cell wall and the spectrum of compounds solubilized from the cell wall can better inform technologies for plant cell wall deconstruction and conversion to renewable fuels and chemicals. The work presented on these technologies will span four areas that include: (1) characterizing how lignin properties (S/G ratio, p-hydroxycinnamic acid content, and total lignin content) and their alteration during alkaline hydrogen peroxide (AHP) pretreatment impacts enzymatic digestibility for grasses with diverse lignin phenotypes, (2) characterizing the impacts of pretreatment on the extractability/accessibility of the non-cellulosic polysaccharides in the cell walls of diverse plants using "glycome profiling" or screening a library of 156 monoclonal antibodies against polysaccharide epitopes, (3) identifying the spectrum of fermentation inhibitors generated by AHP pretreatment of grasses for high-sugar concentration fermentation by xylose-fermenting Saccharomyces cerevisiae strains and demonstration of improved xylose fermentation and hydrolysate tolerance through evolutionary engineering, and (4) quantifying the impact of AHP pretreatment on plant cell wall water swelling capacity and how the water-cell wall environment influences its susceptibility to enzymatic hydrolysis.
Thesis Defence - Harald Aigner: Characterization of FtsH proteases in the annual plant Arabidopsis thaliana
01-Jun-2012 10:00 - 13:00
Harald Aigner
Title:
Characterization of FtsH proteases in the annual plant Arabidopsis thaliana
Opponent: Sacha Baginski, Professor, Institut fur Biochemie und BioTechnologie, Martin Luther Unitersität, Halle Wittenberg.
Supervisor: Christiane Funk
Lilla hörsalen , KB3A9
UPSC Half-time Seminar - Melis Kucukoglu: Molecular Regulation of Vascular Cambium Identity and Activity
04-Jun-2012 15:00 - 16:00
Speaker:
Melis Kucukoglu
Title:
Molecular Regulation of Vascular Cambium Identity and Activity
Place Lilla hörsalen
Seminar Grégory Mouille: The control of cell wall quality during plant development
05-Jun-2012 10:00 - 11:00
Speaker:
Grégory Mouille
Institute JP Bourgin, INRA Versailles, France
Title:
The control of cell wall quality during plant development
Place: Lilla hörsalen KB3A9
Seminar - Georgia Drakakaki: Understanding the relation of endomembrane trafficking and polysaccharide deposition using proteomics and chemical genomics
07-Jun-2012 15:00 - 16:00
Speaker:
Georgia Drakakaki
UC Davis California, USA
Title:
“Understanding the relation of endomembrane trafficking and polysaccharide deposition using proteomics and chemical genomics”
Place: KB3A9 “Lilla hörsalen”, KBC
Host: Stephanie Robert
Presentation of Master Thesis - Patricia Lang
08-Jun-2012 14:00 - 15:00
Patricia Lang
Title:
KREUZ UND QUER gene function during root epidermal patterning in
Arabidopsis thaliana
Supervisor: Markus Grebe
Room: KBF 30, 14.00
UPSC Half-Time Seminar: Malgorzata Pietrzykowska
11-Jun-2012 15:00 - 16:00
Speaker:
Malgorzata Pietrzykowska
Title:
Is there a functional difference between Lhcb1 and Lhcb2?
Place Lilla hörsalen
Seminar - Beverley Green: Algal genomics and the evolution of light-harvesting antennae
12-Jun-2012 14:00 - 15:00
Department of Chemistry
Seminar
Beverley Green
University of British Columbia, Vancouver, Canada
Title: Algal genomics and the evolution of light-harvesting antennae
Host: Christiane Funk
Place: Lilla Hörsalen, KB3A9, KBC
Abstract:
The ocean is the new frontier in genomics research! The marine algae are particularly important because of their influence on global climate as well as their major contribution to the oceanic food web. The recently sequenced genomes of a number of these algae have illuminated the wide range of photosynthetic biodiversity in the ocean. Particularly interesting are the algae with chlorophyll a/c light-harvesting antennas, such as the diatoms and brown algae, which acquired their plastids by secondary endosymbiosis, and have derived new varieties of light-harvesting antennas.
Cutting Edge Seminar - Theodorus Meuwissen: Prediction of total genetic value using whole genome sequence data
13-Jun-2012 10:00 - 11:00
Theodorus Meuwissen
Department of Animal and Aquacultural Sciences
The Norwegian University of Life Sciences (UMB), Ås, Norway
Title:
Prediction of total genetic value using whole genome sequence data
Place: Stora hörsalen, KBC
Seminar - Kazuya Kikuchi: Design, synthesis and biological application of in vivo imaging probes with tunable chemical switches
15-Jun-2012 13:30 - 14:30
Seminar
professor Kazuya Kikuchi
Osaka University
Graduate School of Engineering
WPI-Immunology Frontier Research Center
Osaka, Japan
Title: Design, synthesis and biological application of in vivo imaging probes with tunable chemical switches
Host: Gunnar Öquist
Place: KBC, Large Lecture hall "Stora hörsalen"
Abstract:
One of the great challenges in the post-genome era is to clarify the biological significance of intracellular molecules directly in living cells. If we can image a molecule in action, it is possible to acquire biological information, which is unavailable if we deal with cell homogenates. One possible approach is to design and synthesize chemical probes that can convert biological information to chemical output. Protein fluorescent labeling provides an attractive approach to study the localization and function of proteins in living cells. Recently, a specific pair of a protein tag and its ligand has been utilized to visualize a protein of interest (POI). In this method, a POI is fused with a protein tag and the tag is labeled with the ligand connected to a fluorescent molecule. The advantage of this protein labeling system is that a variety of fluorescent molecules are potentially available as labeling reagents, and that the protein tag is conditionally labeled with its fluorescent ligand. I have designed a protein labeling system that allows fluorophores to be linked to POI. The protein tag (BL-tag) is a mutant class A ?-lactamase (TEM-1) modified to be covalently bound to the designed specific labeling probes and the labeling probes is consisted with a ?-lactam ring (ampicillin, cephalosporin) attached to various fluorophores. A fluorogenetic labeling system can be designed using the unique property of cephalosporin, which release leaving group by subsequent reaction after opening the lactam ring. For further sophisticated application, multicolor imaging was done by adopting the colorful fluorophores.
Seminar - Janne Lehtiö: Defining human proteome using high resolution peptide isoelectric focusing coupled to mass spectrometry (HiRIEF-LC-MS) and the application of this method to improve cancer treatment
15-Jun-2012 15:00 - 16:00
Seminar
Speaker:
Janne Lehtiö
Clinical Proteomics Mass Spectrometry, Karolinska Institutet, SciLifeLab Stockholm
Science for Life Laboratory
Title:
Defining human proteome using high resolution peptide isoelectric focusing coupled to mass spectrometry (HiRIEF-LC-MS) and the application of this method to improve cancer treatment
Place: Lilla hörsalen, KB3A9
Host Anders Nordström
Seminar - Stacey Lee Thompson
18-Jun-2012 15:00 - 16:00
Place: KB3A9 "Lilla hörsalen", KBC
Time: Monday June 18 at 15.00
MSc Thesis seminar - Johanna Carlsson
20-Jun-2012 13:00 - 14:00
Date: Wednesday 20/6
Place: KBF30
Time: 13-14
Seminar - Andreas Grönlund: Modeling intracellular regulation: Transcription factor kinetics
20-Jun-2012 15:00 - 16:00
Speaker: Andreas Grönlund
new assoc. lector in biological modelling at the department
Title: Modeling intracellular regulation: Transcription factor kinetics
Abstract:
Basic physical constraints in how macromolecules are made and how fast they can find each other in the intracellular environment constrain the fidelity of cellular regulation. These two properties are recently studied experimentally as well as analytically. I will give a brief overview of how such processes can be characterized and modeled.
Place: KB3A9 Lilla hörsalen
Cutting Edge Seminar - Takashi Ueda: How plants acquired novel membrane trafficking pathways
23-Aug-2012 15:00
Speaker:
Takashi Ueda
Tokyo University, Japan
Title: How plants acquired novel membrane trafficking pathways
Place: Lilla hörsalen, KB3A9, KBC
UPSC Cutting Edge Seminar - Ueli Grossniklaus:Molecular Control of Fertilization and Interspecific Crossing Barriers
03-Sep-2012 10:00 - 11:00
Speaker: Ueli Grossniklaus, Institute of Plant Biology, University of Zuerich
Title: Molecular Control of Fertilization and Interspecific Crossing Barriers
Place: Lilla hörsalen, KB3A9, KBC
Date: Monday, September 03, 2012 at 10:00
Seminar - Örjan Carlborg: Understanding the genetic architecture of complex traits
04-Sep-2012 15:15 - 16:15
Medical Biochemistry and Biophysics Seminar Series
Speaker:
Örjan Carlborg
Dept. of Clinical Sciences, Division of Computational Genetics, SLU, Uppsala,
Title: Understanding the genetic architecture of complex traits
Host: Andrei Chabes, Medchem
Place: KB3A9, Lilla hörsalen
Abstract:
Understanding how genes contribute to the phenotypic variability observed in populations is a major challenge in biology. A common approach to dissect the genetics of complex traits is to measure the genotype of individuals in a population at a large number of loci across the genome and then evaluate whether the phenotypic mean differs between the individuals that carry particular combinations of genetic variants, alleles, at either individual loci (i.e. detection of additive, dominance and epigenetic effects of loci) or at multiple loci (i.e. to detect genetic interactions or epistasis). I will here give a brief introduction to this topic and illustrate the insights that can be gained into the genetics of complex traits using these approaches by using examples from our research in domestic animals. An alternative, and promising, strategy to identify genes involved in gene-by-gene or gene-by-environment interactions is to search for loci that causes a difference in variance (a variance heterogeneity) between genotypes. This talk will be concluded by presenting some recent work to develop theory and tools for genome-wide mapping of individual variance-controlling loci. Empirical findings from studies of data in Arabidopsis thaliana and Saccharomyces cerevisiae will be used to illustrate the contribution of such loci to the genetic architecture of complex traits and the implications of the findings on our understanding of the genetic regulation of complex trait variation.
Seminar - Tony Bacic: Grass cell walls: Investigating the molecular mechanism of (1,3;1,4)-?-D-glucan biosynthesis
10-Sep-2012 12:00 - 13:00
Professor Tony Bacic
Univ. of Melbourne, Australia
Title: Grass cell walls: Investigating the molecular mechanism of (1,3;1,4)-?-D-glucan biosynthesis
Place:
KB3B3, KBC
Seminar - Jaime Barros-Rios
10-Sep-2012 15:00 - 16:00
Speaker:
Jaime Barros-Rios
Title: Effects of divergent selection for diferulates on cell wall composition, biodegradability, and stem borers resistance in maize
Place: Lilla hörsalen, KBC, KB3A9
Seminar - Wilfred Vermerris: Modifying cell wall composition to enhance the production of biofuels and nanomaterials
12-Sep-2012 10:00 - 11:00
UPSC Seminar
Speaker:
Dr. Wilfred Vermerris
Department of Microbiology & Cell Science
University of Florida Genetics Institute
Gainesville, FL, USA
Title:
Modifying cell wall composition to enhance the production of biofuels and nanomaterials
Place:
Lilla hörsalen, KB3A9, KBC
Host: Hannele Tuominen
The production of fermentable sugars from lignocellulosic biomass is affected by the chemical composition of the cell wall. Lignin content and subunit composition appear to be of particular importance. We have generated and studied several different classes of cell wall mutants of maize and sorghum to define how variation in lignin composition impacts biomass conversion, to identify the underlying genes, and to explain the biochemical basis of enhanced conversion using fluorescently labeled cellulases. The success of the bio-economy also depends on economic and environmental sustainability. I will highlight how the waste stream of the biorefinery can be used to produce nanomaterials as high-value co-products that have the potential to offset the cost of biofuel production.
Dissertation - Lorenz Gerber
14-Sep-2012 10:00 - 11:00
Title: Cell Wall Chemotyping for Functional Genomics. Applications of Pyrolysis-Gas Chromatography / Mass Spectrometry.
Opponent: Professor Shawn Mansfield, Department of Wood Science, The University of British Columbia, UBC, Canada
When and Where: 2012-09-14 10:00, Umeå, KB3A9, KBC huset, Umeå universitet
Seminar (Half-time) - Mir Amir Mahboubi
17-Sep-2012 15:00 - 16:00
Half-time Seminar
Speaker:
Mir Amir Mahboubi
Title: Carbon allocation and partitioning into cell wall polymers
Place: Lilla hörsalen, KB3A9, KBC
Workshop on Plant Proteomics with Focus on Redox Proteomics
19-Sep-2012 14:00 - 16:00
Workshop on Plant Proteomics with Focus on Redox Proteomics
The workshop is organized by the KBC Proteomics Facility in collaboration with the International Plant Proteomics Organization (INPPO).
Speakers:
Ian Max Møller (Aarhus University, Denmark)
Per Hägglund (Technical University of Denmark)
Bob Buchanan (University of California, Berkeley)
Thomas Kieselbach (Umeå University)
The workshop will give an introduction into the chemistry of post-translational protein modifications by carbonylation and thiol oxidation and present methods to study them by proteomics. As for carbonylation, a brief introduction to the analysis of stress-induced ROS production and metal-catalyzed protein oxidation and will be given and the consequences of irreversible protein oxidation will be discussed. As for thiol oxidation different, the workshop will provide an introduction to different techniques to study target proteins of thioredoxin (Trx) using mass spectrometry and gel based approaches. This includes the use of ICAT reagents for Trx-target identification and the analysis of Trx-targets using 2-D fluorescence electrophoresis and affinity chromatography by means of monocysteinic Trx-ligands. The opportunities and limitations of these techniques will be discussed using examples from different research projects.
Please register by sending an email to:
Location: KBF 31
Special Seminar by Bob Buchanan: Video presentation of an interview with Andrew Benson
19-Sep-2012 16:00 - 17:00
Bob Buchanan has kindly offered to present the video of an interview that he recorded some weeks ago with Andrew Benson. The video highlights Andrew Benson's contribution to the discovery of the Benson Calvin cycle, gives credit to less-known persons who made important contributions to the research of Melvin Calvin and gives some interesting insights into how laboratory work looked like in those days. In addition to the video presentation Bob Buchanan offered to give a seminar to introduce the topic. This activity is going to be both a research seminar and a presentation of an interesting chapter of research history.
Place: Lilla hörsalen, KB3A9