Workshop on Plant Proteomics with Focus on Redox Proteomics
The workshop is organized by the KBC Proteomics Facility in collaboration with the International Plant Proteomics Organization (INPPO).
Ian Max Møller (Aarhus University, Denmark)
Per Hägglund (Technical University of Denmark)
Bob Buchanan (University of California, Berkeley)
Thomas Kieselbach (Umeå University)
The workshop will give an introduction into the chemistry of post-translational protein modifications by carbonylation and thiol oxidation and present methods to study them by proteomics. As for carbonylation, a brief introduction to the analysis of stress-induced ROS production and metal-catalyzed protein oxidation and will be given and the consequences of irreversible protein oxidation will be discussed. As for thiol oxidation different, the workshop will provide an introduction to different techniques to study target proteins of thioredoxin (Trx) using mass spectrometry and gel based approaches. This includes the use of ICAT reagents for Trx-target identification and the analysis of Trx-targets using 2-D fluorescence electrophoresis and affinity chromatography by means of monocysteinic Trx-ligands. The opportunities and limitations of these techniques will be discussed using examples from different research projects.
Location: KBF 31