Wood is a major source of renewable raw materials that are widely used in the pulp, paper, and timber industries. Poplar has emerged as the model tree for woody species, and molecular tools have been developed that allow break¬through science in this forest tree. In my research, a major focus has been on proteins that have been shown to play a role in wood development and more specifically in ligno- cellolusic processes. To study this, different proteomics techniques have been explored and developed.
Reactive oxygen species (ROS)ROS have recently been suggested to act as signaling molecules in the control of specialized processes, such as plant growth and defense, hormonal signaling, and development.Within the project, my group has explored the possibility to alter wood quality by the regulation of ROS at the plasmalemma/cell wall interface using transgenic methodology.A special type of superoxide dismutase with a high isoelectric point (hipI-SOD) has been mainly considered for this genetic manipulation due to its possible role as a regulator of hydrogen peroxide in the polymerization of lignin. Thorough characterization of this protein in Poplar has shown that it exists in two isoforms and in an additional spliced form. We are currently using these plants to study the underlying mechanisms of ROS-mediated regulation of cell wall development using multivariate methods in combination with data from metabolomic and transcriptomic platforms.
Linocellulose regulating proteinsIn this project we are taking a proteomic approach to answer fundamental questions about cellulose deposition in the second- ary cell wall that are important for chemical and ultra structural properties of xylem fibers in wood. In our focuses to achieve this goal we will explore the structural composition and function of cellulose synthases (CesAs) in the plasma membrane of wood fibers.The project will highly depend on masspectrometry in combination with multivariate statistics and bioinformatics. Another group of proteins that has been identified as central to the regulation of phenylpropanoid metabolism is the MYB tran- scription factor family.Each MYB transcription factor contains a conserved DNA-binding domain, located in the N-terminal part of the protein.One of the intriguing features of PttMYB21a antisense plants was the presence of lignin compounds with increased degrees of methoxylation. Moreover, elevated levels of caffeoyl CoA 3-O-methyltransferase transcripts were found in the transgenic plants, suggesting that PttMYB21a could act as a transcriptional repressor.
The Mediator and close functional relations between the Mediator modules and other transcriptional regulators (from Oudgenoeg et al. unpulished data)General transcription The MediatorIn all eukaryotes, protein-encoding genes are transcribed by pol II.To perform its most basal functions, pol II requires five general transcription factors (GTFs).It is also now known that a Mediator, a 25 subunit protein complex, functions as a connector between the promoter-bound transcriptional regulators and pol II.The Mediator was first identified in Saccharomyces as an activity required for transcriptional activation in vitro. Later, it was identified as a multiprotein complex that provides an inter- face for activator and repressor proteins to transmit information from regulatory DNA elements to core promoters.A number of studies have determined that the Mediator is composed of three modules designated as "tail", "head", and "middle". It is important for transcription control, modulating the frequency of initiation in responses to both positive and negative regula- tory factors. Surprisingly, prior to our publication (Bäckstrom et al. 2007), there were had been no reports of the Mediator in plants.We are now studying redox mechanisms of this complexr.
Svensk sammanfattning
Key Publications
- Nilsson R, Bernfur K, Gustavsson N, Bygdell J, Wingsle G, Larsson C. (2010) Proteomics of plasma membranes from leaves, xylem, and cambium/phloem of young poplar trees reveals tissue distribution of transporters, receptors, and proteins involved in wood formation. Molecular & Cell Proteomics 9: 368-387
- Srivastava V, Schinkel H, Witzell J, Hertzberg M, Torp M, Srivastava MK, Karpinska B, Melzer M, Wingsle G (2007) Downregu- lation of high-isoelectric-point extracellular superoxide dismutase mediates alterations in the metabolism of reactive oxygen species and developmental disturbances in hybrid aspen. Plant J 49: 135-148
- Bylesjö M, Nilsson R, Srivastava V, Grönlund A, Johansson AI, Jansson S, Karlsson J, Moritz T, Wingsle G, Trygg J. (2009) In- tegrated Analysis of Transcript, Protein and Metabolite Data to Study Lignin Biosynthesis in Hybrid Aspen. J. Prot. Res. 8:199-210.
- Srivastava V, Srivastav MK, Chibani K, Nilsson R, Melzer M, Wingsle G (2009) Alternative Splicing Studies of the ROS Gene Network in Populus Reveals Two Isoforms of High Iso-electric point Superoxide Dismutase. Plant Physiol. 149: 1848-1859.
- Bäckström S, Elfving N, Nilsson R, Wingsle G, Björklund S (2007) Purification of mediator from Arabidopsis thaliana identifies PFT1 as the Med25 subunit. Mol Cell 26: 717-729
- Karpinska B, Karlsson M, Srivastava M, Stenberg A, Schrader J, Sterky F, Bhalerao R, Wingsle G (2004) MYB transcription factors are differentially expressed and regulated during secondary vascular tissue development in hybrid aspen. Plant Molecular Biology 56; 255-270.
CV Dr. Gunnar Wingsle
- 1982: BSc Umeå University
- 1991: Ph.D. Swedish University of Agricultural Sciences
- 1994: Docent, Swedish University of Agricultural Sciences
- 1996: Assistant professor, Swedish University of Agricultural Sciences
- 1998: Director of studies, Swedish University of Agricultural Sciences
- 2001: Professor, Swedish University of Agricultural Sciences
- 2007: Member of Chemistry Board at Umeå University
- 2009: Manager of the KBC proteomic platform at Umeå University and SLU
- 2010: Member of the KBC board at Umeå University and SLU.
Publication list
-
Overexpression of vesicle-associated membrane protein PttVAP27-17 as a tool to improve biomass production and the overall saccharification yields in Populus trees
Biotechnol Biofuels. 2021, 14(1):43
-
A GDSL lipase-like from Ipomoea batatas catalyzes efficient production of 3,5-diCQA when expressed in Pichia pastoris
Commun Biol. 2020, 3(1):673
-
Hunting monolignol transporters: membrane proteomics and biochemical transport assays with membrane vesicles of Norway spruce
J Exp Bot. 2020, 71(20):6379-6395
-
Nuclear proteome analysis of Chlamydomonas with response to CO2 limitation
Algal Research 2020, 46(101765)
-
Targeted Multiple Reaction Monitoring Analysis of CSF Identifies UCHL1 and GPNMB as Candidate Biomarkers for ALS
J Mol Neurosci. 2019, 69(4):643-657
-
Cellulose synthase stoichiometry in aspen differs from Arabidopsis and Norway spruce
Plant Physiology 2018, 177 (3):1096-1107
-
A multi-omics approach reveals function of Secretory Carrier-Associated Membrane Proteins in wood formation of Populus trees
BMC Genomics. 2018, 19(1)
-
The function of two type II metacaspases in woody tissues of Populus trees
New Phytol. 2018, 217(4):1551-1565
-
Protein expression in tension wood formation monitored at high tissue resolution in Populus
J Exp Bot. 2017, 68 (13):3405-3417
-
Quantitative proteomics reveals protein profiles underlying major transitions in aspen wood development
BMC Genomics 2016, 17(1):119
-
Yeast Elongator protein Elp1p does not undergo proteolytic processing in exponentially growing cells
Microbiologyopen. 2015, 4 (6):867-878
-
Redox regulation of the MED28 and MED32 mediator subunits is important for development and senescence
Protoplasma. 2016, 253(3):957-963;
-
Biochemical and redox characterization of the Mediator complex and its associated transcription factor GeBPL, a GLABROUS1 enhancer binding protein
Biochem. J. 2015, 468:385-400
-
OnPLS integration of transcriptomic, proteomic and metabolomic data shows multi-level oxidative stress responses in the cambium of transgenic hipI-superoxide dismutase Populus plants
BMC Genomics. 2013; 14:893
-
The effect of carbohydrates and osmoticum on storage reserve accumulation and germination of Norway spruce somatic embryos
Physiol Plant. 2013; 149(2):273-285
-
Shaikhali J, Barajas-Lopéz J, Ötvös K, Kremnev D, Sánchez Garcia A, Srivastava V, Wingsle G, Bako L, Strand Å
The CRYPTOCHROME1-dependent response to excess light is mediated through the transcriptional activators ZINC FINGER PROTEIN EXPRESSED IN INFLORESCENCE MERISTEM LIKE1 and 2 in Arabidopsis
Plant Cell, 2012, 24(7):3009-25
-
Shaikhali J, Noren L, Barajas-Lopez JD, Srivastava V, Konig J, Sauer UH, Wingsle G, Dietz KJ, Strand A
Redox-mediated mechanisms regulate DNA-binding activity of the G-group of bZIP transcription factors in Arabidopsis.
J Biol Chem. 2012, 287(33):27510-25
-
Chibani K, Tarrago L, Gualberto JM, Wingsle G, Rey P, Jacquot JP, Rouhier N
Atypical Thioredoxins in Poplar: The Glutathione-Dependent Thioredoxin-Like 2.1 Supports the Activity of Target Enzymes Possessing a Single Redox Active Cysteine
Plant Physiol. 2012, 159(2):592-605
-
Selles B, Hugo M, Trujillo M, Srivastava V, Wingsle G, Jacquot JP, Radi R, Rouhier N
Hydroperoxide and peroxynitrite reductase activity of poplar thioredoxin-dependent glutathione peroxidase 5: kinetics, catalytic mechanism and oxidative inactivation
Biochem J. 2012 442(2):369-380
-
Palm ME, Weise CF, Lundin C, Wingsle G, Nygren Y, Björn E, Naredi P, Wolf-Watz M, Wittung-Stafshede P
Cisplatin binds human copper chaperone Atox1 and promotes unfolding in vitro
Proceedings of the National Academy of Sciences of the United States of America: 2011 108:6951-6956
-
Elfving N, Davoine C, Benlloch R, Blomberg J, Brännström K, Müller D, Nilsson A, Ulfstedt M, Ronne H, Wingsle G, Nilsson O, Björklund S
The Arabidopsis thaliana Med25 mediator subunit integrates environmental cues to control plant development
Proceedings of the National Academy of Sciences of the United States of America: 2011 108:8245-8250
-
Bergemalm D, Forsberg K, Srivastava V, Graffmo KS, Andersen PM, Brännström T, Wingsle G, Marklund SL
Superoxide dismutase-1 and other proteins in inclusions from transgenic amyotrophic lateral sclerosis model mice
Journal of Neurochemistry; 2010 114:408-418
-
Nilsson R, Bernfur K, Gustavsson N, Bygdell J, Wingsle G, Larsson C
Proteomics of plasma membranes from poplar trees reveals tissue distribution of transporters, receptors, and proteins in cell wall formation
Molecular & Cellular Proteomics: 2010 9:368-387
-
Yanamandra K, Alexeyev O, Zamotin V, Srivastava V, Shchukarev A, Brorsson A-C, Tartaglia GG, Vogl T, Kayed R, Wingsle G, Olsson J, Dobson CM, Berg A, Elgh F, Morozova-Roche, LA
Amyloid formation by the pro-inflammatory S100A8/A9 proteins in the ageing prostate
PloS One: 2009 4:e5562
-
Chibani K, Wingsle G, Jacquot J-P, Gelhaye E, Rouhier N
Comparative Genomic Study of the Thioredoxin Family in Photosynthetic Organisms with Emphasis on Populus trichocarpa
Molecular Plant: 2009 2(2):308-322
-
Chibani K, Wingsle G, Jacquot J-P, Gelhaye E, Rouhier N
Comparative Genomic Study of the Thioredoxin Family in Photosynthetic Organisms with Emphasis on Populus trichocarpa
Molecular Plant: 2009 2(2):308-322
-
Srivastava V, Srivastava MK, Chibani K, Nilsson R, Rouhier N, Melzer M, Wingsle G
Alternative splicing studies of the reactive oxygen species gene network in Populus reveal two isoforms of high-isoelectric-point superoxide dismutase
Plant Physiology: 2009 149:1848-1859
-
Srivastava V, Srivastava MK, Chibani K, Nilsson R, Rouhier N, Melzer M, Wingsle G
Alternative splicing studies of the reactive oxygen species gene network in Populus reveal two isoforms of high-isoelectric-point superoxide dismutase
Plant Physiology: 2009 149:1848-1859
-
Bylesjö M, Nilsson R, Srivastava V, Grönlund A, Johansson AI, Jansson S, Karlsson J, Moritz T, Wingsle G, Trygg J
Integrated Analysis of Transcript, Protein and Metabolite Data To Study Lignin Biosynthesis in Hybrid Aspen
Journal of Proteome Research: 2009 8:199-210
-
Bylesjö M, Nilsson R, Srivastava V, Grönlund A, Johansson AI, Jansson S, Karlsson J, Moritz T, Wingsle G, Trygg J
Integrated Analysis of Transcript, Protein and Metabolite Data To Study Lignin Biosynthesis in Hybrid Aspen
Journal of Proteome Research: 2008, Published on line
-
Koh CS, Navrot N, Didierjean C, Rouhier N, Hirasawa M, Knaff DB, Wingsle G, Samian R, Jacquot J-P, Corbier C, Gelhaye E
An atypical catalytic mechanism involving three cysteines of thioredoxin
Journal of Biological Chemistry: 2008 283:23062-23072
-
Overmeyer K, Kollist H, Tuominen H, Betz, C, Langebartels C, Wingsle G, Kangasjärvi S, Brader G, Mullineaux P, Kangasjärvi J
Complex phenotypic profiles leading to ozone sensitivity in Arabidopsis thaliana mutants
Plant, Cell and Environment: 2008 31: 1237-1249
-
Overmeyer K, Kollist H, Tuominen H, Betz, C, Langebartels C, Wingsle G, Kangasjärvi S, Brader G, Mullineaux P, Kangasjärvi J
Complex phenotypic profiles leading to ozone sensitivity in Arabidopsis thaliana mutants
Plant, Cell and Environment: 2008 31: 1237-1249
-
Bäckström S, Elfving N, Nilsson R, Wingsle G, Björklund S
Purification of a Plant Mediator from Arabidopsis thaliana Identifies PFT1 as the Med25 Subunit
Molecular Cell: 2007 26:717-729
-
Bäckström S, Elfving N, Nilsson R, Wingsle G, Björklund S
Purification of a Plant Mediator from Arabidopsis thaliana Identifies PFT1 as the Med25 Subunit
Molecular Cell: 2007 26:717-729
-
Martz F, Sutinen M-L, Derome K, Wingsle G, Julkunen-Tiitto R, Turunen M
Effects of ultraviolet (UV) exclusion on the seasonal concentration of photosynthetic and UV-screening pigments in Scots pine needles
Global Change Biology: 2007 13:252-265
-
Martz F, Sutinen M-L, Derome K, Wingsle G, Julkunen-Tiitto R, Turunen M
Effects of ultraviolet (UV) exclusion on the seasonal concentration of photosynthetic and UV-screening pigments in Scots pine needles
Global Change Biology: 2007 13:252-265
-
Srivastava V, Schinkel H, Witzell J, Hertzberg M, Torp M, Srivastava MK, Karpinska B, Melzer M, Wingsle G
Downregulation of high-isoelectric-point extracellular superoxide dismutase mediates alterations in the metabolism of reactive oxygen species and developmental disturbances in hybrid aspen
The Plant Journal: 2007 49:135-148
-
Srivastava V, Schinkel H, Witzell J, Hertzberg M, Torp M, Srivastava MK, Karpinska B, Melzer M, Wingsle G
Downregulation of high-isoelectric-point extracellular superoxide dismutase mediates alterations in the metabolism of reactive oxygen species and developmental disturbances in hybrid aspen
The Plant Journal: 2007 49:135-148
-
Ferreira S, Hjerno K, Larsen M, Wingsle G, Larsen P, Fey S, Roepstorff P, Pais MS
Proteome profiling of Populus euphratica Oliv. Upon heat stress
Annals of Botany: 2006 98:361-377
-
Rouhier N, Gama F, Wingsle G, Gelhaye E, Gans P, Jacquot JP
Engineering functional artificial hybrid proteins between poplar peroxiredoxin II and glutaredoxin or thioredoxin
Biochemical and Biophysical Research Communications: 2006 341:1300-1308
-
Rouhier N, Villarejo A, Srivastava M, Gelhaye E, Keech O, Droux M, Finkemeier I, Samuelsson G, Dietz KJ, Jacquot J-P, Wingsle G
Identification of plant glutaredoxin targets
Antioxid Redox Signal: 2005 7:919-929
-
Hoglund S, Larsson S, Wingsle G
Both hypersensitive and non-hypersensitive responses are associated with resistance in Salix viminalis against the gall midge Dasineura marginemtorquens
J Exp Bot: 2005 56:3215-3222
-
Karlsson M, Melzer M, Prokhorenko I, Johansson T, Wingsle G
Hydrogen peroxide and expression of hipI-superoxide dismutase are associated with the development of secondary cell walls in Zinnia elegans
J Exp Bot: 2005 56:2085-2093
-
Wiklund S, Karlsson M, Antti H, Johnels D, Sjostrom M, Wingsle G, Edlund U
A new metabonomic strategy for analysing the growth process of the poplar tree
Plant Biotechnology Journal: 2005 3:353-362
-
Gelhaye E, Rouhier N, Gerard J, Jolivet Y, Gualberto J, Navrot N, Ohlsson PI, Wingsle G, Hirasawa M, Knaff DB, Wang HM, Dizengremel P, Meyer Y, Jacquot JP
A specific form of thioredoxin h occurs in plant mitochondria and regulates the alternative oxidase (vol 101, pg 14545, 2004)
Proceedings of the National Academy of Sciences of the United States of America: 2005 102:3886-3886
-
Louis J, Ounis A, Ducruet JM, Evain S, Laurila T, Thum T, Aurela M, Wingsle G, Alonso L, Pedros R, Moya I
Remote sensing of sunlight-induced chlorophyll fluorescence and reflectance of Scots pine in the boreal forest during spring recovery
Remote Sensing of Environment: 2005 96:37-48
-
Karpinska B, Karlsson M, Srivastava M, Stenberg A, Schrader J, Sterky F, Bhalerao R, Wingsle G
MYB transcription factors are differentially expressed and regulated during secondary vascular tissue development in hybrid aspen
Plant Mol Biol: 2004 56:255-270
-
Gelhaye E, Rouhier N, Gerard J, Jolivet Y, Gualberto J, Navrot N, Ohlsson P-I, Wingsle G, Hirasawa M, Knaff DB, Wang H, Dizengremel P, Meyer Y, Jacquot J-P
A specific form of thioredoxin h occurs in plant mitochondria and regulates the alternative oxidase
Proc Natl Acad Sci U S A: 2004 101:14545-14550
-
Lovgren JM, Bylund GO, Srivastava MK, Lundberg LAC, Persson OP, Wingsle G, Wikstrom PM
The PRC-barrel domain of the ribosome maturation protein RimM mediates binding to ribosomal protein S19 in the 30S ribosomal subunits
Rna-a Publication of the Rna Society: 2004 10:1798-1812
-
Karpinska B, Karlsson M, Schinkel H, Streller S, Suss KH, Melzer M, Wingsle G
A novel superoxide dismutase with a high isoelectric point in higher plants. Expression, regulation, and protein localization
Plant Physiology: 2001 126:1668-1677
-
Schinkel H, Hertzberg M, Wingsle G
A small family of novel CuZn-superoxide dismutases with high isoelectric points in hybrid aspen
Planta: 2001 213:272-279
-
Karpinska B, Wingsle G, Karpinski S
Antagonistic effects of hydrogen peroxide and glutathione on acclimation to excess excitation energy in Arabidopsis
IUBMB Life: 2000 50:21-26
-
Wingsle G, Karpinski S, Hallgren JE
Low temperature, high light stress and antioxidant defence mechanisms in higher plants
Phyton-Annales Rei Botanicae: 1999 39:253-268
-
Karpinski S, Reynolds H, Karpinska B, Wingsle G, Creissen G, Mullineaux P
Systemic signaling and acclimation in response to excess excitation energy in Arabidopsis
Science: 1999 284:654-657
-
Tao DL, Oquist G, Wingsle G
Active oxygen scavengers during cold acclimation of Scots pine seedlings in relation to freezing tolerance
Cryobiology: 1998 37:38-45
-
Schinkel H, Streller S, Wingsle G
Multiple forms of extracellular superoxide dismutase in needles, stem tissues and seedlings of Scots pine
Journal of Experimental Botany: 1998 49:931-936
-
Wingsle G, Moritz T
Analysis of ascorbate and dehydroascorbate in plant extracts by high-resolution selected ion monitoring gas chromatography mass spectrometry
Journal of Chromatography A: 1997 782:95-103
-
Streller S, Schinkel H, Wingsle G
Apoplasmic CuZn-superoxide dismutase in Pinus sylvestris
Phyton-Annales Rei Botanicae: 1997 37:271-276
-
Krivosheeva A, Tao DL, Ottander C, Wingsle G, Dube SL, Oquist G
Cold acclimation and photoinhibition of photosynthesis in Scots pine
Planta: 1996 200:296-305
-
Wingsle G, Karpinski S
Differential redox regulation by glutathione of glutathione reductase and CuZn-superoxide dismutase gene expression in Pinus sylvestris L needles
Planta: 1996 198:151-157
-
Karpinski S, Karpinska B, Wingsle G, Hallgren JE
Molecular Responses to Photooxidative Stress in Pinus-Sylvestris .1. Differential Expression of Nuclear and Plastid Genes in Relation to Recovery from Winter Stress
Physiologia Plantarum: 1994 90:358-366
-
Streller S, Karpinski S, Hallgren JE, Wingsle G
4 Cytosolic-Type Cuzn-Superoxide Dismutases in Germinating-Seeds of Pinus-Sylvestris
Physiologia Plantarum: 1994 92:443-450
-
Mazzafera P, Wingsle G, Olsson O, Sandberg G
S -Adenosyl -L -Methionine -Theobromine 1 -N -Methyltransferase, an Enzyme Catalyzing the Synthesis of Caffeine in Coffee
Phytochemistry: 1994 37:1577-1584
-
Streller S, Kromer S, Wingsle G
Isolation and Purification of Mitochondrial Mn-Superoxide Dismutase from the Gymnosperm Pinus-Sylvestris L
Plant and Cell Physiology: 1994 35:859-867
-
Streller S, Wingsle G
Pinus-Sylvestris L Needles Contain Extracellular Cuzn Superoxide-Dismutase
Planta: 1994 192:195-201
-
Wingsle G, Hallgren JE
Influence of So2 and No2 Exposure on Glutathione, Superoxide-Dismutase and Glutathione-Reductase Activities in Scots Pine Needles
Journal of Experimental Botany: 1993 44:463-470
-
Karpinski S, Wingsle G, Karpinska B, Hallgren JE
Molecular Responses to Photooxidative Stress in Pinus-Sylvestris (L) .2. Differential Expression of Cuzn-Superoxide Dismutases and Glutathione-Reductase
Plant Physiology: 1993 103:1385-1391
-
Karpinski S, Wingsle G, Karpinska B, Hallgren JE
Differential Expression of Cuzn-Superoxide Dismutases in Pinus-Sylvestris Needles Exposed to So2 and No2
Physiologia Plantarum: 1992 85:689-696
-
Leverenz JW, Oquist G, Wingsle G
Photosynthesis and Photoinhibition in Leaves of Chlorophyll-B-Less Barley in Relation to Absorbed Light
Physiologia Plantarum: 1992 85:495-502
-
Wingsle G, Strand M, Karpinski S, Hallgren JE
Influence of So2 and No2 Exposure on Antioxidants and Photosynthesis in Needles of Pinus-Sylvestris
Phyton-Annales Rei Botanicae: 1992 32:147-153
-
Karpinski S, Wingsle G, Olsson O, Hallgren JE
Characterization of Cdnas Encoding Cuzn-Superoxide Dismutases in Scots Pine
Plant Molecular Biology: 1992 18:545-555
-
Wingsle G, Mattson A, Ekblad A, Hallgren JE, Selstam E
Activities of Glutathione-Reductase and Superoxide-Dismutase in Relation to Changes of Lipids and Pigments Due to Ozone in Seedlings of Pinus-Sylvestris (L)
Plant Science: 1992 82:167-178
-
Rosenqvist E, Wingsle G, Ogren E
Photoinhibition of Photosynthesis in Intact Willow Leaves in Response to Moderate Changes in Light and Temperature
Physiologia Plantarum: 1991 83:390-396
-
Wingsle G, Gardestrom P, Hallgren JE, Karpinski S
Isolation, Purification, and Subcellular-Localization of Isozymes of Superoxide-Dismutase from Scots Pine (Pinus-Sylvestris L) Needles
Plant Physiology: 1991 95:21-28
-
Palmqvist K, Sundblad LG, Wingsle G, Samuelsson G
Acclimation of Photosynthetic Light Reactions During Induction of Inorganic Carbon Accumulation in the Green-Alga Chlamydomonas-Reinhardtii
Plant Physiology: 1990 94:357-366
-
Wingsle G, Sandberg G, Hallgren JE
Determination of Glutathione in Scots Pine Needles by High-Performance Liquid-Chromatography as Its Monobromobimane Derivative
Journal of Chromatography: 1989 479:335-344
-
Wingsle G
Purification and Characterization of Glutathione-Reductase from Scots Pine Needles
Physiologia Plantarum: 1989 76:24-30
-
Wingsle G, Nasholm T, Lundmark T, Ericsson A
Induction of Nitrate Reductase in Needles of Scots Pine-Seedlings by Nox and No3
Physiologia Plantarum: 1987 70:399-403