Wood is a major source of renewable raw materials that are widely used in the pulp, paper, and timber industries. Poplar has emerged as the model tree for woody species, and molecular tools have been developed that allow break¬through science in this forest tree. In my research, a major focus has been on proteins that have been shown to play a role in wood development and more specifically in ligno- cellolusic processes. To study this, different proteomics techniques have been explored and developed.

Gunnar Wingsele 1150
Reactive oxygen species (ROS)

ROS have recently been suggested to act as signaling molecules in the control of specialized processes, such as plant growth and defense, hormonal signaling, and development.Within the project, my group has explored the possibility to alter wood quality by the regulation of ROS at the plasmalemma/cell wall interface using transgenic methodology.A special type of superoxide dismutase with a high isoelectric point (hipI-SOD) has been mainly considered for this genetic manipulation due to its possible role as a regulator of hydrogen peroxide in the polymerization of lignin. Thorough characterization of this protein in Poplar has shown that it exists in two isoforms and in an additional spliced form. We are currently using these plants to study the underlying mechanisms of ROS-mediated regulation of cell wall development using multivariate methods in combination with data from metabolomic and transcriptomic platforms.

Linocellulose regulating proteins
In this project we are taking a proteomic approach to answer fundamental questions about cellulose deposition in the second- ary cell wall that are important for chemical and ultra structural properties of xylem fibers in wood. In our focuses to achieve this goal we will explore the structural composition and function of cellulose synthases (CesAs) in the plasma membrane of wood fibers.The project will highly depend on masspectrometry in combination with multivariate statistics and bioinformatics. Another group of proteins that has been identified as central to the regulation of phenylpropanoid metabolism is the MYB tran- scription factor family.Each MYB transcription factor contains a conserved DNA-binding domain, located in the N-terminal part of the protein.One of the intriguing features of PttMYB21a antisense plants was the presence of lignin compounds with increased degrees of methoxylation. Moreover, elevated levels of caffeoyl CoA 3-O-methyltransferase transcripts were found in the transgenic plants, suggesting that PttMYB21a could act as a transcriptional repressor.
wingsle 1The Mediator and close functional relations between the Mediator modules and other transcriptional regulators (from Oudgenoeg et al. unpulished data)
General transcription The Mediator
In all eukaryotes, protein-encoding genes are transcribed by pol II.To perform its most basal functions, pol II requires five general transcription factors (GTFs).It is also now known that a Mediator, a 25 subunit protein complex, functions as a connector between the promoter-bound transcriptional regulators and pol II.The Mediator was first identified in Saccharomyces as an activity required for transcriptional activation in vitro. Later, it was identified as a multiprotein complex that provides an inter- face for activator and repressor proteins to transmit information from regulatory DNA elements to core promoters.A number of studies have determined that the Mediator is composed of three modules designated as "tail", "head", and "middle". It is important for transcription control, modulating the frequency of initiation in responses to both positive and negative regula- tory factors. Surprisingly, prior to our publication (Bäckstrom et al. 2007), there were had been no reports of the Mediator in plants.We are now studying redox mechanisms of this complexr.
sweden_greySvensk sammanfattning

Publication list

  1. Cellulose synthase stoichiometry in aspen differs from Arabidopsis and Norway spruce
    Plant Physiology 2018, 177 (3):1096-1107
  2. A multi-omics approach reveals function of Secretory Carrier-Associated Membrane Proteins in wood formation of​ ​​Populus​​ ​trees
    BMC Genomics. 2018, 19(1)
  3. The function of two type II metacaspases in woody tissues of Populus trees
    New Phytol. 217 (4):1551-1565
  4. Protein expression in tension wood formation monitored at high tissue resolution in Populus
    J Exp Bot. 2017, 68 (13):3405-3417
  5. Quantitative proteomics reveals protein profiles underlying major transitions in aspen wood development
    BMC Genomics 2016, 17(1):119
  6. Yeast Elongator protein Elp1p does not undergo proteolytic processing in exponentially growing cells
    Microbiologyopen. 2015, 4 (6):867-878
  7. Redox regulation of the MED28 and MED32 mediator subunits is important for development and senescence
    Protoplasma. 2016, 253(3):957-963;
  8. Biochemical and redox characterization of the Mediator complex and its associated transcription factor GeBPL, a GLABROUS1 enhancer binding protein
    Biochem. J. 2015, 468:385-400
  9. OnPLS integration of transcriptomic, proteomic and metabolomic data shows multi-level oxidative stress responses in the cambium of transgenic hipI-superoxide dismutase Populus plants
    BMC Genomics. 2013; 14:893
  10. Businge E, Bygdell J, Wingsle G, Moritz T, Egertsdotter U
    The effect of carbohydrates and osmoticum on storage reserve accumulation and germination of Norway spruce somatic embryos
    Physiol Plant. 2013; 149(2):273-285
  11. Shaikhali J, Barajas-Lopéz J, Ötvös K, Kremnev D, Sánchez Garcia A, Srivastava V, Wingsle G, Bako L, Strand Å
    The CRYPTOCHROME1-dependent response to excess light is mediated through the transcriptional activators ZINC FINGER PROTEIN EXPRESSED IN INFLORESCENCE MERISTEM LIKE1 and 2 in Arabidopsis
    Plant Cell, 2012, 24(7):3009-25
  12. Shaikhali J, Noren L, Barajas-Lopez JD, Srivastava V, Konig J, Sauer UH, Wingsle G, Dietz KJ, Strand A
    Redox-mediated mechanisms regulate DNA-binding activity of the G-group of bZIP transcription factors in Arabidopsis.
    J Biol Chem. 2012, 287(33):27510-25
  13. Chibani K, Tarrago L, Gualberto JM, Wingsle G, Rey P, Jacquot JP, Rouhier N
    Atypical Thioredoxins in Poplar: The Glutathione-Dependent Thioredoxin-Like 2.1 Supports the Activity of Target Enzymes Possessing a Single Redox Active Cysteine
    Plant Physiol. 2012, 159(2):592-605
  14. Selles B, Hugo M, Trujillo M, Srivastava V, Wingsle G, Jacquot JP, Radi R, Rouhier N
    Hydroperoxide and peroxynitrite reductase activity of poplar thioredoxin-dependent glutathione peroxidase 5: kinetics, catalytic mechanism and oxidative inactivation
    Biochem J. 2012 442(2):369-380
  15. Palm ME, Weise CF, Lundin C, Wingsle G, Nygren Y, Björn E, Naredi P, Wolf-Watz M, Wittung-Stafshede P
    Cisplatin binds human copper chaperone Atox1 and promotes unfolding in vitro
    Proceedings of the National Academy of Sciences of the United States of America: 2011 108:6951-6956
  16. Elfving N, Davoine C, Benlloch R, Blomberg J, Brännström K, Müller D, Nilsson A, Ulfstedt M, Ronne H, Wingsle G, Nilsson O, Björklund S
    The Arabidopsis thaliana Med25 mediator subunit integrates environmental cues to control plant development
    Proceedings of the National Academy of Sciences of the United States of America: 2011 108:8245-8250
  17. Bergemalm D, Forsberg K, Srivastava V, Graffmo KS, Andersen PM, Brännström T, Wingsle G, Marklund SL
    Superoxide dismutase-1 and other proteins in inclusions from transgenic amyotrophic lateral sclerosis model mice
    Journal of Neurochemistry; 2010 114:408-418
  18. Nilsson R, Bernfur K, Gustavsson N, Bygdell J, Wingsle G, Larsson C
    Proteomics of plasma membranes from poplar trees reveals tissue distribution of transporters, receptors, and proteins in cell wall formation
    Molecular & Cellular Proteomics: 2010 9:368-387
  19. Yanamandra K, Alexeyev O, Zamotin V, Srivastava V, Shchukarev A, Brorsson A-C, Tartaglia GG, Vogl T, Kayed R, Wingsle G, Olsson J, Dobson CM, Berg A, Elgh F, Morozova-Roche, LA
    Amyloid formation by the pro-inflammatory S100A8/A9 proteins in the ageing prostate
    PloS One: 2009 4:e5562
  20. Chibani K, Wingsle G, Jacquot J-P, Gelhaye E, Rouhier N
    Comparative Genomic Study of the Thioredoxin Family in Photosynthetic Organisms with Emphasis on Populus trichocarpa

    Molecular Plant: 2009 2(2):308-322
  21. Srivastava V, Srivastava MK, Chibani K, Nilsson R, Rouhier N, Melzer M, Wingsle G
    Alternative splicing studies of the reactive oxygen species gene network in Populus reveal two isoforms of high-isoelectric-point superoxide dismutase
    Plant Physiology: 2009 149:1848-1859
  22. Bylesjö M, Nilsson R, Srivastava V, Grönlund A, Johansson AI, Jansson S, Karlsson J, Moritz T, Wingsle G, Trygg J
    Integrated Analysis of Transcript, Protein and Metabolite Data To Study Lignin Biosynthesis in Hybrid Aspen
    Journal of Proteome Research: 2009 8:199-210
  23. Koh CS, Navrot N, Didierjean C, Rouhier N, Hirasawa M, Knaff DB, Wingsle G, Samian R, Jacquot J-P, Corbier C, Gelhaye E
    An atypical catalytic mechanism involving three cysteines of thioredoxin
    Journal of Biological Chemistry: 2008 283:23062-23072
  24. Overmeyer K, Kollist H, Tuominen H, Betz, C, Langebartels C, Wingsle G, Kangasjärvi S, Brader G, Mullineaux P, Kangasjärvi J
    Complex phenotypic profiles leading to ozone sensitivity in Arabidopsis thaliana mutants
    Plant, Cell and Environment: 2008 31: 1237-1249
  25. Bäckström S, Elfving N, Nilsson R, Wingsle G, Björklund S
    Purification of a Plant Mediator from Arabidopsis thaliana Identifies PFT1 as the Med25 Subunit
    Molecular Cell: 2007 26:717-729
  26. Martz F, Sutinen M-L, Derome K, Wingsle G, Julkunen-Tiitto R, Turunen M
    Effects of ultraviolet (UV) exclusion on the seasonal concentration of photosynthetic and UV-screening pigments in Scots pine needles
    Global Change Biology: 2007 13:252-265
  27. Srivastava V, Schinkel H, Witzell J, Hertzberg M, Torp M, Srivastava MK, Karpinska B, Melzer M, Wingsle G
    Downregulation of high-isoelectric-point extracellular superoxide dismutase mediates alterations in the metabolism of reactive oxygen species and developmental disturbances in hybrid aspen
    The Plant Journal: 2007 49:135-148
  28. Rouhier N, Gama F, Wingsle G, Gelhaye E, Gans P, Jacquot JP
    Engineering functional artificial hybrid proteins between poplar peroxiredoxin II and glutaredoxin or thioredoxin
    Biochemical and Biophysical Research Communications: 2006 341:1300-1308
  29. Ferreira S, Hjerno K, Larsen M, Wingsle G, Larsen P, Fey S, Roepstorff P, Pais MS
    Proteome profiling of Populus euphratica Oliv. Upon heat stress
    Annals of Botany: 2006 98:361-377
  30. Wiklund S, Karlsson M, Antti H, Johnels D, Sjostrom M, Wingsle G, Edlund U
    A new metabonomic strategy for analysing the growth process of the poplar tree
    Plant Biotechnology Journal: 2005 3:353-362
  31. Gelhaye E, Rouhier N, Gerard J, Jolivet Y, Gualberto J, Navrot N, Ohlsson PI, Wingsle G, Hirasawa M, Knaff DB, Wang HM, Dizengremel P, Meyer Y, Jacquot JP
    A specific form of thioredoxin h occurs in plant mitochondria and regulates the alternative oxidase (vol 101, pg 14545, 2004)
    Proceedings of the National Academy of Sciences of the United States of America: 2005 102:3886-3886
  32. Hoglund S, Larsson S, Wingsle G
    Both hypersensitive and non-hypersensitive responses are associated with resistance in Salix viminalis against the gall midge Dasineura marginemtorquens
    J Exp Bot: 2005 56:3215-3222
  33. Karlsson M, Melzer M, Prokhorenko I, Johansson T, Wingsle G
    Hydrogen peroxide and expression of hipI-superoxide dismutase are associated with the development of secondary cell walls in Zinnia elegans
    J Exp Bot: 2005 56:2085-2093
  34. Rouhier N, Villarejo A, Srivastava M, Gelhaye E, Keech O, Droux M, Finkemeier I, Samuelsson G, Dietz KJ, Jacquot J-P, Wingsle G
    Identification of plant glutaredoxin targets
    Antioxid Redox Signal: 2005 7:919-929
  35. Louis J, Ounis A, Ducruet JM, Evain S, Laurila T, Thum T, Aurela M, Wingsle G, Alonso L, Pedros R, Moya I
    Remote sensing of sunlight-induced chlorophyll fluorescence and reflectance of Scots pine in the boreal forest during spring recovery
    Remote Sensing of Environment: 2005 96:37-48
  36. Gelhaye E, Rouhier N, Gerard J, Jolivet Y, Gualberto J, Navrot N, Ohlsson P-I, Wingsle G, Hirasawa M, Knaff DB, Wang H, Dizengremel P, Meyer Y, Jacquot J-P
    A specific form of thioredoxin h occurs in plant mitochondria and regulates the alternative oxidase
    Proc Natl Acad Sci U S A: 2004 101:14545-14550
  37. Karpinska B, Karlsson M, Srivastava M, Stenberg A, Schrader J, Sterky F, Bhalerao R, Wingsle G
    MYB transcription factors are differentially expressed and regulated during secondary vascular tissue development in hybrid aspen
    Plant Mol Biol: 2004 56:255-270
  38. Lovgren JM, Bylund GO, Srivastava MK, Lundberg LAC, Persson OP, Wingsle G, Wikstrom PM
    The PRC-barrel domain of the ribosome maturation protein RimM mediates binding to ribosomal protein S19 in the 30S ribosomal subunits
    Rna-a Publication of the Rna Society: 2004 10:1798-1812
  39. Karpinska B, Karlsson M, Schinkel H, Streller S, Suss KH, Melzer M, Wingsle G
    A novel superoxide dismutase with a high isoelectric point in higher plants. Expression, regulation, and protein localization
    Plant Physiology: 2001 126:1668-1677
  40. Schinkel H, Hertzberg M, Wingsle G
    A small family of novel CuZn-superoxide dismutases with high isoelectric points in hybrid aspen
    Planta: 2001 213:272-279
  41. Karpinska B, Wingsle G, Karpinski S
    Antagonistic effects of hydrogen peroxide and glutathione on acclimation to excess excitation energy in Arabidopsis
    IUBMB Life: 2000 50:21-26
  42. Wingsle G, Karpinski S, Hallgren JE
    Low temperature, high light stress and antioxidant defence mechanisms in higher plants
    Phyton-Annales Rei Botanicae: 1999 39:253-268
  43. Karpinski S, Reynolds H, Karpinska B, Wingsle G, Creissen G, Mullineaux P
    Systemic signaling and acclimation in response to excess excitation energy in Arabidopsis
    Science: 1999 284:654-657
  44. Tao DL, Oquist G, Wingsle G
    Active oxygen scavengers during cold acclimation of Scots pine seedlings in relation to freezing tolerance
    Cryobiology: 1998 37:38-45
  45. Schinkel H, Streller S, Wingsle G
    Multiple forms of extracellular superoxide dismutase in needles, stem tissues and seedlings of Scots pine
    Journal of Experimental Botany: 1998 49:931-936
  46. Wingsle G, Moritz T
    Analysis of ascorbate and dehydroascorbate in plant extracts by high-resolution selected ion monitoring gas chromatography mass spectrometry
    Journal of Chromatography A: 1997 782:95-103
  47. Streller S, Schinkel H, Wingsle G
    Apoplasmic CuZn-superoxide dismutase in Pinus sylvestris
    Phyton-Annales Rei Botanicae: 1997 37:271-276
  48. Krivosheeva A, Tao DL, Ottander C, Wingsle G, Dube SL, Oquist G
    Cold acclimation and photoinhibition of photosynthesis in Scots pine
    Planta: 1996 200:296-305
  49. Wingsle G, Karpinski S
    Differential redox regulation by glutathione of glutathione reductase and CuZn-superoxide dismutase gene expression in Pinus sylvestris L needles
    Planta: 1996 198:151-157
  50. Streller S, Karpinski S, Hallgren JE, Wingsle G
    4 Cytosolic-Type Cuzn-Superoxide Dismutases in Germinating-Seeds of Pinus-Sylvestris
    Physiologia Plantarum: 1994 92:443-450
  51. Streller S, Kromer S, Wingsle G
    Isolation and Purification of Mitochondrial Mn-Superoxide Dismutase from the Gymnosperm Pinus-Sylvestris L
    Plant and Cell Physiology: 1994 35:859-867
  52. Karpinski S, Karpinska B, Wingsle G, Hallgren JE
    Molecular Responses to Photooxidative Stress in Pinus-Sylvestris .1. Differential Expression of Nuclear and Plastid Genes in Relation to Recovery from Winter Stress
    Physiologia Plantarum: 1994 90:358-366
  53. Streller S, Wingsle G
    Pinus-Sylvestris L Needles Contain Extracellular Cuzn Superoxide-Dismutase
    Planta: 1994 192:195-201
  54. Mazzafera P, Wingsle G, Olsson O, Sandberg G
    S -Adenosyl -L -Methionine -Theobromine 1 -N -Methyltransferase, an Enzyme Catalyzing the Synthesis of Caffeine in Coffee
    Phytochemistry: 1994 37:1577-1584
  55. Wingsle G, Hallgren JE
    Influence of So2 and No2 Exposure on Glutathione, Superoxide-Dismutase and Glutathione-Reductase Activities in Scots Pine Needles
    Journal of Experimental Botany: 1993 44:463-470
  56. Karpinski S, Wingsle G, Karpinska B, Hallgren JE
    Molecular Responses to Photooxidative Stress in Pinus-Sylvestris (L) .2. Differential Expression of Cuzn-Superoxide Dismutases and Glutathione-Reductase
    Plant Physiology: 1993 103:1385-1391
  57. Wingsle G, Mattson A, Ekblad A, Hallgren JE, Selstam E
    Activities of Glutathione-Reductase and Superoxide-Dismutase in Relation to Changes of Lipids and Pigments Due to Ozone in Seedlings of Pinus-Sylvestris (L)
    Plant Science: 1992 82:167-178
  58. Karpinski S, Wingsle G, Olsson O, Hallgren JE
    Characterization of Cdnas Encoding Cuzn-Superoxide Dismutases in Scots Pine
    Plant Molecular Biology: 1992 18:545-555
  59. Karpinski S, Wingsle G, Karpinska B, Hallgren JE
    Differential Expression of Cuzn-Superoxide Dismutases in Pinus-Sylvestris Needles Exposed to So2 and No2
    Physiologia Plantarum: 1992 85:689-696
  60. Wingsle G, Strand M, Karpinski S, Hallgren JE
    Influence of So2 and No2 Exposure on Antioxidants and Photosynthesis in Needles of Pinus-Sylvestris
    Phyton-Annales Rei Botanicae: 1992 32:147-153
  61. Leverenz JW, Oquist G, Wingsle G
    Photosynthesis and Photoinhibition in Leaves of Chlorophyll-B-Less Barley in Relation to Absorbed Light
    Physiologia Plantarum: 1992 85:495-502
  62. Wingsle G, Gardestrom P, Hallgren JE, Karpinski S
    Isolation, Purification, and Subcellular-Localization of Isozymes of Superoxide-Dismutase from Scots Pine (Pinus-Sylvestris L) Needles
    Plant Physiology: 1991 95:21-28
  63. Rosenqvist E, Wingsle G, Ogren E
    Photoinhibition of Photosynthesis in Intact Willow Leaves in Response to Moderate Changes in Light and Temperature
    Physiologia Plantarum: 1991 83:390-396
  64. Palmqvist K, Sundblad LG, Wingsle G, Samuelsson G
    Acclimation of Photosynthetic Light Reactions During Induction of Inorganic Carbon Accumulation in the Green-Alga Chlamydomonas-Reinhardtii
    Plant Physiology: 1990 94:357-366