I am interested in carbohydrate metabolism of plants. In particular I wish to understand how car- bohydrate metabolism is coupled to cell wall bio- synthesis, especially to the biosynthesis of cellu- lose. The majority of the biomass accumulation on the planet occurs in the cell walls of non-photo- synthetic plant tissues such as the wood of trees. This biomass resource provides the main source of biopolymers in the world and its importance will increase in the future as global demand for renewable materials and fuels increases.

Totte Niittyla 1150The work in my group addresses genetic factors contributing to stem biomass and wood density.We focus on the molecular mechanisms responsible for carbon allocation to developing wood of trees.The carbon in wood is mostly found in three main cell wall polymers; cellulose, hemicelluloses and lignin. In most plant species the majority of this carbon is derived from sucrose imported from photosynthetic tissues.Therefore understanding
of sucrose transport to wood and subsequent production of cell wall polymer precursors is central for understanding factors controlling stem biomass and wood density. Our research on carbon allocation to wood is mostly done with hybrid aspen.

totte_1 Arabidopsis stem 880
Light microscopy picture of aspen wood fibers and vessels. Cross section of Arabidopsis stem. Lignified cell walls are shown in red and non-lignified in blue.
Aspen stem 880 Embyo seed 880
 Cross section of aspen stem.  Developing Arabidopsis seed. Wild type seed and developing embryo (left), and a putative sugar signaling mutant showing defects in embryo development (right).

In addition to identifying the components of sucrose to cell wall polymer pathways we are interested in how sugar metabolism is regulated in plants, especially in relation to sugar availability.To this end we work on the protein phosphorylation and protein trafficking responses communicating information about the sugar status of plant cells and organs, and how this information is translated to the responses at the cellular and whole plant level. Protein phosphorylation–dephosphorylation and protein trafficking are fundamental principles in the regu- lation of many biological responses in all organisms, including the primary carbohydrate metabolism of plants. In this research we use Arabidopsis seedlings and the developing embryo as model systems.
In our work we use tools from molecular biology, bio- chemistry, genetics, microscopy, isotope flux analysis and mass spectrometry.

sweden_greySvensk sammanfattning

Publication list

  1. Cellulose synthase stoichiometry in aspen differs from Arabidopsis and Norway spruce
    Plant Physiology 2018, 177 (3):1096-1107
  2. Sucrose transport and carbon fluxes during wood formation
    Physiologia Plantarum 2018, 164(1):67-81
  3. Two Complementary Mechanisms Underpin Cell Wall Patterning during Xylem Vessel Development
    Plant Cell. 2017, 29 (10):2433-2449
  4. AspWood: High-spatial-resolution transcriptome profiles reveal uncharacterized modularity of wood formation in Populus tremula
    Plant Cell. 2017, 29 (7):1585-1604
  5. Spatially resolved metabolic analysis reveals a central role for transcriptional control in carbon allocation to wood
    J Exp Bot 2017, 68 (13):3529-3539
  6. Laser Capture Microdissection Protocol for Xylem Tissues of Woody Plants
    Front. Plant Sci., 04 January 2017
  7. Cytosolic invertase contributes to the supply of substrate for cellulose biosynthesis in developing wood
    New Phytol. 2017, 214(2):796-807
  8. Carbon-13 tracking after 13CO2 supply revealed diurnal patterns of wood formation in aspen
    Plant Physiol. 2015; 168(2):478-489
  9. Deficient sucrose synthase activity in developing wood does not specifically affect cellulose biosynthesis, but causes an overall decrease in cell wall polymers
    New Phytol. 2014, 203(4):1220-1230
  10. Aspen SUCROSE TRANSPORTER 3 allocates carbon into wood fibers
    Plant Physiology 2013; 163(4):1729-1740
  11. The Norway spruce genome sequence and conifer genome evolution
    Nature 2013; 497(7451):579-584
  12. Xue W, Ruprecht C, Street N, Hematy K, Chang C, Frommer WB, Persson S, Niittylä T
    Paramutation-Like Interaction of T-DNA Loci in Arabidopsis
    PLoS ONE 2012 7(12): e51651
  13. Roach M, Gerber L, Sandquist D, Gorzsás A, Hedenström M, Kumar M, Steinhauser MC, Feil R, Daniel G, Stitt M, Sundberg B, Niittylä T
    Fructokinase is required for carbon partitioning to cellulose in aspen wood
    Plant Journal, 2012, 70(6):967 – 977
  14. Niittylä T, Chauduri B, Sauer U and Frommer WB
    Comparison of quantitative metabolite imaging tools and carbon-13 techniques for fluxomics
    Methods Mol Biol, 2009, 553:355-372
  15. Chaudhuri B, Niittylä T, Hörmann F, Frommer WB
    Fluxomics with ratiometric metabolite dyes
    Plant Signal Behav. 2007, 2(2):120-2
  16. Niittylä T, Fuglsang AT, Palmgren MG, Frommer WB, Schulze WX
    Temporal analysis of sucrose-induced phosphorylation changes in plasma membrane proteins of Arabidopsis
    Mol Cell Proteomics, 2007,6:1711-1726
  17. Niittylä T, Comparot-Moss S, Lue W-L, Messerli G, Trevisan M, Seymour MD, Gatehouse JA, Villadsen D, Smith SM, Chen J, Zeeman SC, Smith AM
    Similar protein phosphatases control starch metabolism in plants and glycogen metabolism in mammals
    J. Biol. Chem. 2006, 281:11815-11818
  18. Niittylä T, Messerli G, Trevisan M, Chen J, Smith AM, Zeeman SC
    A previously unknown maltose transporter essential for starch degradation in leaves
    Science 2004, 303:87-89
  19. Smith AM, Zeeman SC, Niittylä T, Kofler H, Thorneycroft D, Smith SM
    Starch degradation in leaves
    J. Appl. Glycosci. 2003, 50:173-176
  20. Zabela MD, Fernandez-Delmond I, Niittylä T, Sanchez P, Grant M
    Differential expression of genes encoding Arabidopsis phospholipases after challenge with virulent or avirulent Pseudomonas isolates
    Mol. Plant Microbe Interact. 2002, 15:808-816