SCHENGEN receptor module drives localized ROS production and lignification in plant roots
EMBO J. 2020 Mar 18:e103894 [Epub ahead of print]
Fujita S, De Bellis D, Edel KH, Köster P, Andersen TG, Schmid-Siegert E, Dénervaud Tendon V, Pfister A, Marhavý P, Ursache R, Doblas VG, Barberon M, Daraspe J, Creff A, Ingram G, Kudla J, Geldner N

Abstract
Production of reactive oxygen species (ROS) by NADPH oxidases (NOXs) impacts many processes in animals and plants, and many plant receptor pathways involve rapid, NOX-dependent increases of ROS. Yet, their general reactivity has made it challenging to pinpoint the precise role and immediate molecular action of ROS. A well-understood ROS action in plants is to provide the co-substrate for lignin peroxidases in the cell wall. Lignin can be deposited with exquisite spatial control, but the underlying mechanisms have remained elusive. Here, we establish a kinase signaling relay that exerts direct, spatial control over ROS production and lignification within the cell wall. We show that polar localization of a single kinase component is crucial for pathway function. Our data indicate that an intersection of more broadly localized components allows for micrometer-scale precision of lignification and that this system is triggered through initiation of ROS production as a critical peroxidase co-substrate.

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