Profiling of embryonic nuclear vs. cellular RNA in Arabidopsis thaliana
Genom Data 2015 4: 96-98
Slane D, Kong J, Schmid M, Jurgens G, Bayer M
In Arabidopsis, various cell type-specific whole-genome expression analyses have been conducted. However, the vast majority of these were performed with cellular RNA from root tissues or other easily accessible cell types . Nuclear RNA was neglected for a long time as not being representative for transcriptomic studies. In recent years, however, there have been reports describing the validity of nuclear RNA for these types of studies [2,3]. Here we describe the generation, quality assessment and analysis of nuclear transcriptomic data from Arabidopsis embryos published by Slane et al. (2014) . Comparison of nuclear with cellular gene expression demonstrated the usefulness of nuclear transcriptomics.
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