Ashelford K, Eriksson ME, Allen CM, D'Amore L, Johansson M, Gould P, Kay S, Millar AJ, Hall N, Hall A
Full genome re-sequencing reveals a novel circadian clock mutation in Arabidopsis
Genome Biology: 2011 12:R28, 12 pp

Map based cloning in Arabidopsis thaliana can be a difficult and time-consuming process, specifically if the phenotype is subtle and scoring labour intensive. Here, we have re-sequenced the 120 Mb genome of a novel Arabidopsis clock mutant early bird (ebi-1) in Wassilewskija (Ws-2). We demonstrate the utility of sequencing a backcrossed line in limiting the number of SNP considered. We identify a SNP in the gene AtNFXL-2 as the likely cause of the ebi-1 phenotype.

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