Zheng BS, Rönnberg E, Virtanen L, Salminen TA, Lundgren K, Moritz T, Edqvist J
Arabidopsis sterol carrier protein-2 is required for normal development of seeds and seedlings
Journal of Expreimental Botany: 2008 59(12):3485-3499
The Arabidopsis thaliana sterol carrier protein-2 (AtSCP2) isa small, basic and peroxisomal protein that in vitro enhancesthe transfer of lipids between membranes. AtSCP2 and all otherplant SCP-2 that have been identified are single-domain polypeptides,whereas in many other eukaryotes SCP-2 domains are expressedin the terminus of multidomain polypeptides. The AtSCP2 transcriptis expressed in all analysed tissues and developmental stages,with the highest levels in floral tissues and in maturing seeds.The expression of AtSCP2 is highly correlated with the multifunctionalprotein-2 (MFP2) involved in Î²-oxidation. A. thaliana Atscp2-1plants deficient in AtSCP2 show altered seed morphology, a delayedgermination, and are dependent on an exogenous carbon sourceto avoid a delayed seedling establishment. Metabolomic investigationsrevealed 110 variables (putative metabolites) that differedin relative concentration between Atscp2-1 and normal A. thalianawild-type seedlings. Microarray analysis revealed that manygenes whose expression is altered in mutants with a deficiencyin the glyoxylate pathway, also have a changed expression levelin Atscp2-1.
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