Dolezal K, Astot C, Hanus J, Holub J, Peters W, Beck E, Strnad M, Sandberg G
Identification of aromatic cytokinins in suspension cultured photoautotrophic cells of Chenopodium rubrum by capillary liquid chromatography/frit - fast atom bombardment mass spectrometry
Plant Growth Regulation: 2002 36:181-189
Two previously unrecorded endogenous cytokinin metabolites, 6-[2-(beta-D-glucopyranosyloxy)benzylamino]purine and 6-[2-(beta-D-glucopyranosyloxy)benzylamino]-2-methylthiopurine, were identified, together with 6-benzylamino-9-beta-D-glucopyranosylpurine (BAP9G), from Chenopodium rubrum cells, grown autotrophically in suspension culture. The new metabolites belong to the aromatic class of cytokinins, in which an aromatic side chain is attached at the N-6-position of the adenine species. The identification was performed by capillary-liquid chromatography/frit-fast atom bombardment - mass spectrometry (LC/FAB MS) after pre-column derivatisation and the structural elucidation was confirmed by organic synthesis. Cytokinin activity of the compounds was tested in an Amaranthus bioassay. The endogenous synthesis of the identified compounds was verified by in vivo deuterium labelling of the analysed cytokinin species, thereby for the first time providing absolute evidence for the endogenous origin of these compounds.
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