Nurani G, Eriksson M, Knorpp C, Glaser E, Franzen LG
Homologous and heterologous protein import into mitochondria isolated from the green alga Chlamydomonas reinhardtii
Plant Molecular Biology: 1997 35:973-980
We have established a homologous system for studying mitochondrial protein import in Chlamydomonas reinhardtii, using C. reinhardtii precursor proteins and mitochondria isolated from C. reinhardtii. The precursors of the F-1 alpha ATP synthase subunit and the Rieske FeS protein were imported into mitochondria with high efficiency, while the F-1 beta subunit precursor was imported with much lower efficiency. The import of heterologous precursor proteins from higher plants was also less efficient. The precursor of the C. reinhardtii PsaF chloroplast protein was converted into a protease-protected form upon incubation with mitochondria. In vitro processing studies revealed that in contrast to the situation in higher plants, the processing of the precursors was catalysed by a soluble, matrix-located peptidase.
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