Streller S, Schinkel H, Wingsle G
Apoplasmic CuZn-superoxide dismutase in Pinus sylvestris
Phyton-Annales Rei Botanicae: 1997 37:271-276
An apoplasmic CuZn-superoxide dismutase (SOD, EC 1.15.1.1) was found in needles of Scots pine. The enzyme was isolated from extracellular washing fluid (EWF), and is a novel isoenzyme since its NH2-terminal amino acid sequence differs from that of a previously described apoplasmic isoform. The inclusion of 0.3 M KBr in the extraction buffer of EWF doubled the amount of active SOD extracted from needles, which suggests an ionic binding of this apoplasmic SOD in Scots pine. This apoplasmic SOD was also detected in EWF obtained from extraxylematic tissue (phloem and cambium) while it was absent in xylematic tissue.
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