Elomaa P, Honkanen J, Puska R, Seppanen P, Helariutta Y, Mehto M, Kotilainen M, Nevalainen L, Teeri TH
Agrobacterium-Mediated Transfer of Antisense Chalcone Synthase Cdna to Gerbera-Hybrida Inhibits Flower Pigmentation
Bio-Technology: 1993 11:508-511
We have developed a regeneration method and an Agrobacterium-mediated transformation protocol for the commercially important ornamental plant Gerbera hybrida. Pieces of petioles of the red variety Terra Regina were cocultivated with a disarmed Agrobacterium tumefaciens vector containing a nearly full-length antisense cDNA encoding gerbera chalcone synthase under the control of CaMV 35S promoter, and a nos-nptII marker gene. The transformed cells were selected on the basis of kanamycin resistance and regenerated into flowering plants. Introduction of the antisense cDNA blocked anthocyanin synthesis and resulted in a dramatically altered flower pigmentation in some of the transformants.
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