Ostin A, Monteiro AM, Crozier A, Jensen E, Sandberg G
Analysis of Indole- 3- Acetic- Acid Metabolites from Dalbergia- Dolichopetala by High- Performance Liquid- Chromatography Mass-Spectrometry
Plant Physiology: 1992 100:63-68
A mixture of [2-C-14(1)] and [C-13(6)]indole-3-acetic acid was applied to the cotyledons of 6-day-germinated seeds of "jacaranda do cerrado" (Dalbergia dolichopetala) and after 8 hours the seeds were extracted. Analysis of the fractionated extract by reversed-phase high performance liquid chromatography-radiocounting revealed the presence of five radiolabeled metabolite peaks (I-V). After further purification, the individual peaks of radioactivity were analyzed by combined high performance liquid chromatography-steel filter-fast atom bombardment-mass spectrometry. The metabolite fraction V was found to contain [C-14(1),C-13(6)]indole-3-acetylaspartic acid and unlabeled indole-3-acetylglutamic acid. Analysis of the metabolite fraction II revealed the presence of dioxindole-3-acetylaspartic acid and putative dioxindole-3-acetylglutamic acid as well as putative benzene ring-hydroxylated derivatives of oxindole-3-acetylaspartic acid and oxindole-3-acetylglutamic acid. There was no evidence of significant incorporation of label from [2'-C-14(1)] or [C-13(6)]indole-3-acetic acid into any of these conjugated indoles.
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