Falk S, Samuelsson G
Recovery of Photosynthesis and Photosystem-Ii Fluorescence in Chlamydomonas-Reinhardtii after Exposure to 3 Levels of High Light
Physiologia Plantarum: 1992 85:61-68
Recovery from 60 min of photoinhibitory treatment at photosynthetic photon flux densities of 500, 1400 and 2200-mu-mol m-2 s-1 was followed in cells of the green alga Chlamydomonas reinhardtii grown at 125-mu-mol m-2 s-1. These light treatments re resent photoregulation, moderate photoinhibition and strong photoinhibition, respectively. Treatment in photoregulatory light resulted in an increased maximal rate of oxygen evolution (P(max)) and an increased quantum yield (PHI), but a 15% decrease in F(V)/F(M). Treatment at moderately photoinhibitory light resulted in a 30% decrease in F(V)/F(M) and an approximately equal decrease in PHI. Recovery in dim light restored F(V)/F(M) within 15 and 45 min after high light treatment at 500 and 1 400-mu-mol m-2 s-1 respectively. Convexity (THETA), a measure of the extent of co-limitation between PS II turnover and whole-chain electron transport, and PHI approached, but did not reach the control level during recovery after exposure to 1 400-mu-mol m 2 s-1, whereas P(max) increased above the control. Treatment at 2200-mu-mol m-2 s-1 resulted in a strong reduction of the modeled parameters PHI, THETA and P(max). Subsequent recovery was initially rapid but the rate decreased, and a complete recovery was not reached within 120 min. Based on the results, it is hypothesized that exposure to high light results in two phenomena. The first, expressed at all three light intensities, involves redistribution within the different aspects of PS II heterogeneity rather than a photoinhibitory destruction of PS II reaction centers. The second, most strongly expressed at 2200-mu-mol m-2 s-1, is a physical damage to PS II shown as an almost total loss of PS II(alpha) and PS II Q(B)-reducing centers. Thus recovery displayed two phases, the first was rapid and the only visible phase in algae exposed to 500 and 1 400-mu-mol m-2 s-1. The second phase was slow and visible only in the later part of recovery in cells exposed to 2 200-mu-mol m-2 s-1.
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