Scientific coordinator:
Gunnar Wingsle, This email address is being protected from spambots. You need JavaScript enabled to view it.

Contact: Thomas Kieselbach, This email address is being protected from spambots. You need JavaScript enabled to view it.

The proteomic platform is a part of the Umeå Protein Analysis (UPA) Facility that was initiated in 2003 and provides infrastructure for research in protein science and proteomics. The facility is located at the Chemistry and Biology Centre (KBC) and UPSC. The work of the UPA facility has had a broad perspective from the beginning, covering the entire range of life sciences research in Umeå. As a result of work that combines education, collaborative research and services, substantial advances in proteomics and mass spectrometry-based protein science hashave been achieved. A complete proteomics workflow is available, covering 2-D DIGE automated spot picking and protein analysis using MALDI-TOF and ESI-Q-TOF mass spectrom- etry. The bioinformatics infrastructure includes resources for on-line booking of instruments, data transfer and a lo- cal Mascot server that provides access to many important sequence databases. Instrumentation for the analysis of post-translational modifications (an electron dissociation mass spectrometer) has recently been obtained and will considerably improve the current opportunities for research in protein science.
proteomics6Education is an important part of the work of the UPA facility, for example within the KBC Research School. Researchers and PhD students who have no experience in mass spectrometric analysis of proteins, but would like to use basic techniques in their project, are offered training courses with a theoretical introduction, individual labora- tory training and a practical task.
One important focus for the UPSC proteomic platform has been to create a Populus protein database, which now contains data on almost 6000 proteins from different sub- samples of various tissues. Using this database, it is now possible to profile the protein expression in this tree species. This technique has been extended to parallel monitoring of proteins (proteomics), transcripts (transcriptomics) and biochemical processes (metabolomics) in global profiling. The integration of these huge amounts of data is one of the key challenges in studies of postgenomic functional genomics.


For more information about this facility, please, follow the link below.

playProteomics Facility