CRISPR (Clustered Regulatory Interspaced Short Palindromic Repeats) is a recently discovered method allowing target specific genome editing in a broad range of species. It offers great hopes for the creation of point mutations or gene deletions in plant species for which genetic modification is otherwise challenging. Spruce, as a plant of large economical interest for Swedish industry, is one of them. However, setting up such a method for this perennial species represent unprecedented challenges needing to be overcome. In this project, the student will make use of the already established protocol to generate spruce protoplasts to adjust the conditions required to make CRISPR editing possible in this species.
Supervisor: Ove Nilsson, Ulrika Egertsdotter, Dept of Forest Genetics and Plant Physiology, SLU.