Pulse-amplitude modulated (PAM) fluorometry is one of the most common techniques used to study the photosynthetic electron transfer chain in different physiological conditions. The Dual-PAM-100 measuring system (Heinz Walz GmbH; www.walz.com) is suited for Photosystem II analysis via chlorophyll fluorescence and the state of Photosystem I can be accessed via dual-wavelength P700 absorbance measurements.  Chlorophyll fluorescence and P700 pulse-modulated measurements are synchronized for minimal mutual disturbance, allowing the simultaneous assessment of different photosynthesis parameters via fluorescence and absorbance measurements. The light-induced formation of the trans-thylakoid proton gradient, ΔpH can be evaluated via 9-amino acridine fluorescence.

The Dual-PAM-100 provides many measuring routines for optimal assessment of photosynthetic parameters. All essential light sources are integrated into the system: red or blue fluorescence excitation light, P700 measuring light, single turnover saturating flashes, actinic light and far-red light. All of these can be switched within 2.5 µs time resolution.  A wide range of measuring light frequencies (1 Hz to 400 kHz) ensure the recording of fast kinetics. The measuring head with integrated light sources is compact and easy to handle.
For a review on Chl fluorescence see: Maxwell K. and Johnson G., J. Exp. Bot. (2000) 51, 659–668,  Kalaji H. et al, Photosynth Res (2017) 132, 13–66Kramer D et al, Photosynth. Res. (2004) 79, 209-218.

Dual-PAM-100 Measuring System

Location: room B3.44.49