Month Flat Week Day

Mon. 14 Oct, 2019

UPSC Monday Seminar 2019

Mon. 14 Oct, 2019 9:00 - 10:00
UPSC Monday Seminarseries 2019

9:00 Chanaka Mannapperuma
Department of Plant Physiology

Title: An update on PlantGenIE and integrating the PLAZA resource

Supervisor: Nathaniel Street

9:30 Maxime Chantreau
Department of Plant Physiology

Title: Regulation of the contrasted lignification pattern between vessels and neighboring cells

Supervisor: Hannele Tuominen

Place: Lilla hörsalen
Time: 9:00-10:00

Contact: Anne Honsel

Departmental meeting genfys (SLU) (APT möte)

Mon. 14 Oct, 2019 10:15 - 11:15

Tue. 15 Oct, 2019

There are no events on this day.

Wed. 16 Oct, 2019

There are no events on this day.

Thu. 17 Oct, 2019

UPSC Seminar: Paul Hudson

Thu. 17 Oct, 2019 14:00 - 15:00
UPSC Seminar

Paul Hudson
Systems Biology, KTH, Stockholm, Sweden

Title: Synthetic and systems biology of cyanobacteria empowered with the CRISPRi tool

Host: Åsa Strand


More information about Paul Hudson's research: https://www.kth.se/profile/huds


Abstract for the talk:
Our group is using the inducible gene-repression tool CRISPRi in Synechocystis for multiple projects in metabolic engineering and systems biology. We have exploited the multiplex capability (6 sgRNAs) of CRISPRi to rapidly screen the effect of eliminating competing pathways on fatty alcohol production. In this case, we observed regulatory “compensation” when repressing genes around the acyl-ACP node. An inducible CRISPRi allows controlled repression of essential genes, and we have used repression of citrate synthase to arrest growth and direct flux to lactate, where specific productivities are increased several fold. I will discuss how sgRNA libraries, combined with droplet-based cell sorting for lactate secretion, can be used to find better strategies for arresting growth while keeping CO2 fixation high. Finally, I will give an example of how we use CRISPRi repression of enzymes to force accumulation of a metabolite, and perform subsequent transcriptomics to uncover metabolite-responsive gene regulation.

Fri. 18 Oct, 2019

There are no events on this day.

Sat. 19 Oct, 2019

SLU-dagen / SLU Open house

Sat. 19 Oct, 2019 12:00 - 15:00
More information can be found here (only in Swedish):

https://www.slu.se/ew-kalender/2019/10/slu-dagen/

Sun. 20 Oct, 2019

There are no events on this day.