Home  Resources Genomics Facility
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UPSC Genomics Lab |
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Introduction
The Genomics lab at UPSC has been set up to implement technology and resources generated through a large scale Populus EST sequencing project This project, performed as a collaborative effort between UPSC Genomics lab and the Royal Institute of Technology (KTH), has generated over 100 000 Populus EST sequences. The Genomics lab has a DNA sequence facility, where you can leave template and primer and we run the sequences for you. Except for sequencing, you can also hire us to do other labwork and we have a lot of modern lab equipment.
Included in the Genomics lab is a facility which provides DNA Microarray technology for UPSC personnel and guests.
The work of the Genomics lab is supported mainly by the Knut and Alice Wallenberg Foundation and the Foundation for Strategic Research.
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DNA sequencing facility |
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We currently provide capillary sequencing using Beckman CEQ 2000 XL [ http://www.beckman.com ]. The procedure how to prepare samples for sequencing can be found by clicking [Read more] below:
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Read more... [DNA sequencing facility]
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Microarray Facility |
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The Microarray lab is located at floor 3 (in the C part). There you can perform quality control of you RNA, labelling of RNA, hybridisations of arrays etc. We also have two Scan Array scanners (Perkin Elmer)which together with the Genepix or Genespring software provide a powerful, but easy to use, platform for initial micro array analysis.
The Genomics lab provide two types of arrays:
POP2 – a 25k array of Populus cDNAs
CATMA – a 25k array of Arabidopsis GSTs
Users of POP2 arrays (the PIs of the group) need to fill in a request form before they are allowed to use any slides. This is done electronically on the UPSC-BASE , our local BASE site. Here you also fill in all data about your experiments.
The CATMA project is funded by Wallenberg Consortium North .
There are various protocols available for the analysis of microarrays. Some currently used at UPSC are found here.
The Genomics lab has a BioRobotics MicroGrid II robot, capable of printing over 100 slides/run using samples stored in a temperature controlled biobank. Using a 24 pin tool, the robot is able to produce high density slide arrays.
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DNA Stocks |
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cDNA clones, sequences/stocks
The large scale EST sequencing of Populus clones has generated over 100000 ESTs. These sequences can be found in the Populus DB.
We have all these clones as glycerol stocks, plasmids or lysates in our freezers. We also have 19 different cDNA libraries [Excel File] from Populus.
If you are interested in a particular clone or a cDNA library, contact Susanne or Thomas and they will help you to find/pick them. Unfortunately, we do not have resources to distribute clones to researchers outside the Populus EST sequencing project.
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Organisation and Contact Information |
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Organization
Three people are currently working in the Genomics lab: Thomas Hiltonen (Research engineer, Head of the lab), Susanne Larsson (Research engineer) and Karin Degerman (Laboratory engineer). There is also a steering committee (Stefan Jansson, Jan Karlsson, Rishi Bhalerao and Ove Nilsson) overseeing the work of this facility and deciding what projects will be done.
Contact information
Steering committee:
Staff:
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