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Plant Cell Wall and Carbohydrate Analysis |
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Plant cell walls and glycobiology are important aspects of UPSC research. The UPSC/KBC Plant Cell Wall and Carbohydrate Analytical Facility is dedicated to these research areas. Equip- ment and competence for applying a large range of standard methods have been established, and 100 m2 of lab space offers enough room for extensive sample preparation equipment, permanently installed equipment for wet-chemical reference methods, and state-of-the-art analytical instrument setups.
 The established conventional methods include many dif- ferent protocols to analyze monosaccharide composition of a whole sample or sample fractions, determination of linkage structure by methylation analysis and several approaches for the fractionation of cell walls. The instrumental backbone for many of those methods is GC/MS(FID). The facility offers one instrument for method development and analytical pyrolysis, while a second is permanently available for sugar analysis.
Ion chromatography (IC) is available for the analysis of monosaccharides without derivatization, as well as oligosaccharides that can be collected for further characterization. Fractions collected after separation by IC or size exclusion chromatography (SEC) can be characterized by electrospray mass spectrometry. An MSn-capable ion-trap instrument for quick determination of carbohydrate structures is installed in the lab. For characterizing a wide variety of polymers, there
is an analytical SEC setup with a quadruple detector array for multi-angle laser light scattering (MALLS), viscometry, refrac- tive index (RI) and UV absorbance detection.
A special field of competence is the analysis of wood and secondary xylem. Carbohydrate composition, lignin and carbohydrate structures, molecular size distributions of poly- mers, cellulose crystallinity and cell wall porosity are some of the parameters that can be analysed. Classical wet chemistry methods have been optimised, in order to reduce the amounts of samples required. Currently, wet chemistry analysis of 2 mg (dry weight) wood samples is feasible. The availability of parallel cell wall analytical methodologies for both model systems (Arabidopsis and Populus) facilitate and accelerate the research process. Many advanced instrumental techniques, such as focal plane array FTIR imaging, 2D solution NMR, CP/ MAS NMR, Py-GC/MS and SEC-MALLS, are used readily for in-depth characterization of wood.
For the growing glycobiology community, the UPSC/KBC Plant Cell Wall and Carbohydrate Analytical Facility offers capabilities to apply proven standard methods and a full range of analytical equipment for in-depth characterization of glycosylated molecules.
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