Figure 2: Real-time imaging of tracheary element differentiation. 35S::Histone-2A-GFP transgenic cell lines induced to be tracheary elements are followed for both GFP tagged nucleus presence coupled to brightfield morphology on the left panel and cell wall fluorescent signal using wheat-germ agglutinin coupled to Alexa633 on the right. 1 frame is taken every 10min during 33h allowing to sequentially observe cell elongation [0-350min], secondary cellulose deposition [390-1760min] and programmed cell death [1760-1770min]. Note the extensive cyclosis (cytoplasmic streaming) during the differentiation causing extreme nuclear movement.

Figure 1: 3D confocal reconstruction of the cell wall of fully differentiated in vitro tracheary element. Reconstruction were made using xyz stack of tracheary element lignin autofluorescent using a Leica SP2 confocal microscope.

Key publications

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1. Pesquet E, Korolev AV, Calder G, Lloyd CW
   Mechanisms for shaping, orienting, positioning and patterning plant secondary cell walls
   Plant Signaling & Behavior: 2011 6:843-849
   
2. Pesquet E, Lloyd C
   Microtubules, MAPs and Xylem Formation
   In: The Plant Cytoskeleton, Advances in Plant Biology 2, Chapter 13 (Edited by B. Liu), pp 227-306. Springer Science+Business Media, LLC
   
3. Pesquet E, Tuominen H
   Ethylene stimulates tracheary element differentiation in Zinnia elegans cell cultures
   New Phytologist: 2011 190:138-149
   
4. Keech O, Pesquet E, Gutierrez L, Ahad A, Bellini C, Smith SM, Gardeström P
   Leaf senescence Is accompanied by an early disruption of the microtubule network in Arabidopsis
   Plant Physiology: 2010 154:1710-1720
   
5. Buschmann H, Sambade A, Pesquet E, Calder G, Lloyd CW
   Microtubule dynamics in plant cells
   Methods in Cell Biology: 2010 97:373-400
   
6. Pesquet E, Korolev AV, Calder G, Lloyd CW
   The microtubule-associated protein AtMAP70-5 regulates secondary wall patterning in Arabidopsis wood cells
   Current Biology: 2010 20:744-749
   
7. Courtois-Moreau CL, Pesquet E, Sjödin A, Muniz L, Bollhöner B, Kaneda M, Samuels L, Jansson S, Tuominen H
   A unique program for cell death in xylem fibers of Populus stem
   The Plant Journal: 2009 58(2):260-274
   
8. Endo S, Pesquet E, Tashiro G, Udagawa-Motose M, Kubo M, Fukuda H, Demura T
   Identifying new components participating in the secondary cell wall formation of vessel elements in Zinnia and Arabidopsis
   The Plant Cell: 2009 21:1155-1165
   
9. Endo S, Pesquet E, Tashiro G, Kuriyama H, Goffner D, Fukuda H, Demura T
   Transient transformation and RNA silencing in Zinnia tracheary element differentiating cell cultures
   The Plant Journal: 2008 53:864-875
   
10. Muñiz L, Minguet EG, Singh SK, Pesquet E, Vera-Sirera F, Moreau-Courtois CL, Carbonell J, Blázquez MA, Tuominen H
    ACAULIS5 controls Arabidopsis xylem specification through the prevention of premature cell death
    Development: 2008 135:2573-2582
    
11. Keech O, Pesquet E, Ahad A, Askne A, Nordvall D, Vodnala SM, Tuominen H, Hurry V, Dizengremel P, Gardeström P
    The different fates of mitochondria and chloroplasts during dark-induced senescence in Arabidopsis leaves
    Plant, Cell and Environment: 2007 30:1523-1534
    
12. Ankele E, Kindgren P, Pesquet E, Strand Å
    In Vivo visualization of Mg-ProtoporphyrinIX, a coordinator of photosynthetic gene expression in the nucleus and the chloroplast
    The Plant Cell: 2007 19:1964-1979
    
13. Pesquet E, Jauneau A, Goffner D
    Zinnia elegans is an excellent model for xylogenesis: from in vitro and in planta
    In Floriculture, Ornamental and Plant Biotechnology: Advances and Topical Issues, Jaime A Teixeira da Silva editor, Global Science Books, Ltd. (London, UK), 171-178
    
14. Beňová-Kákošová A, Digonnet C, Goubet F, Philippe R, Jauneau A, Pesquet E, Barbier O, Zhang Z, Dupree P, Lišková D, Goffner D
    Galactoglucomannans increase cell population density and alter the protoxylem/metaxylem tracheary element ratio in xylogenic cultures of Zinnia
    Plant Physiology: 2006 142:696-709
    
15. Pesquet E, Ranocha P, Legay S, Digonnet C, Barbier O, Pichon M, Goffner D
    Novel markers of xylogenesis in Zinnia are differentially regulated by auxin and cytokinin
    Plant Physiology: 2005 139:1821-1839
    
16. van Raemdonck D, Pesquet E, Cloquet S, Beeckman H, Boerjan W, Goffner D, El Jaziri M, Baucher M
    Molecular changes associated with the setting up of secondary growth in aspen
    Journal of Experimental Botany: 2005 56:221-227
    
17. Pesquet E, Barbier O, Ranocha P, Jauneau A, Goffner D
    Multiple gene detection by in situ RT-PCR in isolated plant cells and tissues
    The Plant Journal: 2004 39:947-959
    
18. Tournier B, Sanchez-Ballesta MT, Jones B, Pesquet E, Regad F, Latche A, Pech J-C, Bouzayen M
    New members of the tomato ERF family show specific expression pattern and diverse DNA-binding capacity to the GCC box element
    FEBS Letters: 2003 550:149-154
    
19. Pesquet E, Jauneau A, Digonnet C, Boudet AM, Pichon M, Goffner D
    Zinnia elegans: the missing link from in vitro tracheary elements to xylem
    Physiologia Plantarum: 2003 119:463-468
    
20. Pesquet E, Pichon M, Pineau C, Ranocha P, Digonnet C, Jauneau A, Boudet AM, Fukuda H, Demura T, Goffner D
    Xylem formation and lignification in trees and model species
    In: Molecular breeding of Woody plant, N.Morohoshi and A. Komamine, editors, Elsevier Science B.V.
    Progress in Biotechnology: 2009 18:11-18