Publications
Ingvarsson PK
Natural selection on synonymous and non-synonymous mutations shape patterns of polymorphism in Populus tremula
Molecular Biology and Evolution: 2010 27:650-660
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Boutté Y, Frescatada-Rosa M, Men S, Chow C-M, Ebine K, Gustavsson A, Johansson L, Ueda T, Moore I, Jürgens G, Grebe M
Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis
The EMBO Journal: 2010 29:546-558
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Lan T, Yang ZL, Yang X, Liu YJ; Wang ZR, Zeng QY
Extensive functional diversification of the Populus glutathione S-transferase supergene family
The Plant Cell: 2009 21:3749-3766
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Elo A, Immanen J, Nieminen K, Helariutta Y
Stem cell function during plant vascular development
Seminars in Cell & Developmental Biology: 2009 20:1097-1106
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Home  Research Publications 2010 Proteomics of plasma membranes from poplar trees reveals tissue distribution of transporters, receptors, and proteins in cell wall formation
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Nilsson R, Bernfur K, Gustavsson N, Bygdell J, Wingsle G, Larsson C
Proteomics of plasma membranes from poplar trees reveals tissue distribution of transporters, receptors, and proteins in cell wall formation
Molecular & Cellular Proteomics: 2010 9:368-387
Abstract
By exploiting the abundant tissues available from Populus trees, 3–4 m high, we have been able to isolate plasma membranes of high purity from leaves, xylem, and cambium/phloem at a time (4 weeks after bud break) when photosynthesis in the leaves and wood formation in the xylem should have reached a steady state. More than 40% of the 956 proteins identified were found in the plasma membranes of all three tissues and may be classified as "housekeeping" proteins, a typical example being P-type H+-ATPases. Among the 213 proteins predicted to be integral membrane proteins, transporters constitute the largest class (41%) followed by receptors (14%) and proteins involved in cell wall and carbohydrate metabolism (8%) and membrane trafficking (8%). ATP-binding cassette transporters (all members of subfamilies B, C, and G) and receptor-like kinases (four subfamilies) were two of the largest protein families found, and the members of these two families showed pronounced tissue distribution. Leaf plasma membranes were characterized by a very high proportion of transporters, constituting almost half of the integral proteins. Proteins involved in cell wall synthesis (such as cellulose and sucrose synthases) and membrane trafficking were most abundant in xylem plasma membranes in agreement with the role of the xylem in wood formation. Twenty-five integral proteins and 83 soluble proteins were exclusively found in xylem plasma membranes, which identifies new candidates associated with cell wall synthesis and wood formation. Among the proteins uniquely found in xylem plasma membranes were most of the enzymes involved in lignin biosynthesis, which suggests that they may exist as a complex linked to the plasma membrane.
e-link to journal
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