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Mapping of ADP-glucose pyrophosphorylase genes from barley |
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Kilian A, Kleinhofs A, Villand P, Thorbjørnsen T, Olsen O-A, Kleczkowski LA
Mapping of ADP-glucose pyrophosphorylase genes from barley.
Theoretical and Applied Genetics: 1994 87:869-871
Abstract:
cDNA probes encoding the
barley endosperm ADP-glucose pyrophosphorylase (AGP) small subunit
(bepsF2), large subunit (bep110), and leaf AGP large subunit (b1p1)
were hybridized with barley genomic DNA blots to determine copy number
and polymerphism. Probes showing polymorphism were mapped on a barley
RFLP map. Probes that were not polymorphic were assigned to chromosome
arms using wheat-barley telosomic addition lines. The data suggested
the presence of a single-copy gene corresponding to each of the cDNA
probes. In addition to the major bands, several weaker
cross-hybridizing bands indicated the presence of other, related
sequences. The weaker bands were specific to each probe and were not
due to crosshybridization with the other probes examined here. The
endosperm AGP small subunit (bepsF2) major band locus was associated
with chromosome 1P and designated Aga1. The endosperm AGP large subunit
(bep110) major-band locus was mapped to chromosome 5M and designated
Aga7. The endosperm AGP large-subunit minor bands were not mapped. The
leaf AGP large-subunit major band was associated with chromosome 7M and
designated Aga5. One of the leaf AGP large-subunit minor bands was
mapped to chromosome 5P and designated Aga6. A clone for the wheat
endosperm AGP large-subunit (pAga7) hybridized to the same barley
genomic DNA bands as the corresponding barley probe indicating a high
degree of identity between the two probes.
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UPSC på Svenska 
Publications 1994 
