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Sub-cellular analysis of lignin synthesis in xylem vessels.


Project description
Lignin is an aromatic cell wall polymer that is one of the main limiting factors determining the quality of plant biomass for conversion to bioethanol and pulp. The present research proposal aims to understand lignin biosynthesis at the sub-cellular level of a distinct lignifying cell type: the tracheary elements (TEs), the cells forming xylem vessels in whole plants. As experimental accessibility to xylem vessels in planta is limited, an in vitro cell culture hormonally inducible into TEs has been optimized, providing a biological system to better understand TE ontogenesis. The post-doctoral fellow will perform protein localisation, over-expression and silencing of target genes implicated in lignin synthesis. In each case, the impact on lignification will be estimated using both microscopical and biochemical (wood chemistry, saccharification) techniques.
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