HomeExam Projects The mediator complex organization in Poplar- a proteomic approach- Gunnar Wingsle
The mediator complex organization in Poplar- a proteomic approach- Gunnar Wingsle
Supervisor: Gunnar Wingsle, Department of Forest Genetics and Plant Physiology, SLU Tel. 786 60 61. E-mail:
The Mediator complex is a part of the general transcription machinery that’s also includes polymerase II in eukaryotes . It composes of three main modules, the tail, middle and head and consists of approximately of 30 proteins all together. This complex has so far only been studied in a few articles from the plant system and this study aims to get basic information of the complex from the xylem tissue from Poplar. The main tool will be to use masspectrometry techniques to identify the specific components in the complex. One aim in the project will also to identify phosphorylation sites in some of the Mediator proteins.